The most common aeroallergens in a tropical region in Southwestern Iran

Assarehzadegan, Mohammad-Ali; Shakurnia, Abdolhussein; Amini, Akram

doi:10.1186/1939-4551-6-7

Cited by 41 publications

(48 citation statements)

References 32 publications

(66 reference statements)

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“…In recent years, the pollen of Acacia species such as A. farnesiana has been recognised as a cause of respiratory allergy in various parts of the Southern hemisphere [1, 4, 6, 7, 27–31]. Up to now, only one allergen (Aca f 2) of Acacia pollen has ever been identified as a member of the profilin family [8].…”

Section: Discussionmentioning

confidence: 99%

“…Up to now, only one allergen (Aca f 2) of Acacia pollen has ever been identified as a member of the profilin family [8]. In this study a cDNA encoding another major allergen from Acacia pollen has been cloned, expressed, and identified as a member of the Ole e 1-like protein family, and its importance has been analysed with a population of patients living Ahvaz city, Southwest Iran, a tropical region in which Acacia pollen induces nearly 48% of the total sensitisation of patients with allergic rhinitis [4]. This allergenic member of the Ole e 1-like protein family of A. farnesiana was named Aca f 1 in accordance with the International Union of Immunological Societies (IUIS) Allergen Nomenclature Subcommittee (http://www.allergen.org/).…”

Section: Discussionmentioning

confidence: 99%

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Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen

Khosravi¹,

Assarehzadegan²,

Morakabati³

et al. 2016

cejoi

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Acacia farnesiana is the main source of allergenic pollen and one of the most important causes of respiratory allergic disease in tropical and subtropical regions of the world. The purpose of this study was to produce a recombinant variety of allergenic Ole e 1-like protein from the pollen of this tree. To predict its allergenic cross-reactivity with other members of the Ole e 1-like protein family of common allergenic plants, the nucleotide sequence homology of the Acacia Ole e 1-like protein was evaluated. Amplification of cDNA strands encoding Acacia Ole e 1-like protein was performed by polymerase chain reaction (PCR) and sequenced. Following expression in Escherichia coli using the pET-21b(+) vector, the recombinant protein was purified using metal-affinity chromatography. IgE-binding competence of purified recombinant Ole e 1- like protein (rAca f 1) was analysed by immunoassay using 25 sera collected from Acacia pollen-sensitised patients. Nucleotide sequencing revealed an open reading frame of 453 bp encoding 150 amino acid residues that belonged to the Ole e 1-like protein family, and 11 patients (44%) had considerable specific IgE levels for the rAca f 1. Immunodetection and inhibition assays indicated that the purified rAca f 1 may be the same as that in the crude extract. Aca f 1, the second allergen from Acacia pollen, was identified as a member of the family of Ole e 1-like protein. A high degree of homology was found among amino acid sequences of Aca f 1 and several allergenic members of Ole e 1-like protein family.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen

Khosravi¹,

Assarehzadegan²,

Morakabati³

et al. 2016

cejoi

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“…13 Many studies from hot and humid regions reported a high prevalence of mites allergy such as in Singapore, Malaysia and Thailand. [14][15][16] Similar conditions are found in Jeddah city with high prevalence of mites reactivity according to the present study.…”

Section: Discussionmentioning

confidence: 99%

“…In a study conducted in Iran, Prosopis juliflora pollen was the most common sensitizing tree pollen followed by pollen of Fraxinus americana tree. 13 Type of allergen pollen seemed to be dependent on the geographical and ecological distributions of trees.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and severity of sensitization reaction to food and inhalant allergens among allergic patients from Jeddah city: Saudi Arabia

Al-Rabia

2016

Int J Community Med Public Health

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“…Several studies have reported on the aeroallergen sensitivity of Iranian patients with asthma or rhinitis [5-7,32], and they found different prevalence rates among atopic subjects and different percentages of allergen sensitization. However, all of these studies agree that pollen sensitization is the most prevalent and that Chenopodiaceae, grass and sycamore are the most important allergenic sources.…”

Section: Discussionmentioning

confidence: 99%

Identification of the main allergen sensitizers in an Iran asthmatic population by molecular diagnosis

Teifoori

Shams-Ghahfarokhi

Postigo

et al. 2014

All Asth Clin Immun

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BackgroundThere has been a significant growth in the prevalence of allergy, mainly associated to IgE-mediated disorders such as asthma and rhinitis. The identification of atopy in asthmatic patients through the measurement of specific IgE can help to identify risk factors that cause asthmatic symptoms in patients. The development and use of individualized allergen-based tests by the Component Resolved Diagnosis has been a crucial advance in the accurate diagnosis and control of allergic patients. The objective of this work was to assess the usefulness of molecular diagnosis to identify environmental allergens as possible factors influencing the development and manifestation of asthma in a group of asthmatic patients from Iran.MethodsStudied population: 202 adult asthmatic patients treated at the Loghman Hakim Hospital and Pasteur Institute of Teheran (Iran) from 2011 to 2012. Specific IgE determined by the ImmunoCAP system were used to both evaluate the patients’ atopic condition and the molecules involved in the allergic sensitization. SDS-PAGE IgE-immunoblotting associated with mass spectrometry was carried out to study the cockroach IgE-binding sensitizing proteins.ResultsForty-five percent of all patients could be considered atopic individuals. Eighty-two percent of atopic patients were sensitized to pollen allergens. The Salsola kali (Sal k 1) and the Phleum pratense (rPhl p 1 and/or rPhl p 5) major allergens were the most common sensitizers among pollens (71% and 18%, respectively). Thirty-five percent of the atopic population was sensitized to cockroach. Four different allergens, including a previously unknown alpha-amylase, were identified in the cockroach extract. No significant associations could be demonstrated between the severity of asthma and the specific IgE levels in the atopic population. Statistical analysis identified the Sal k 1 as the main protein allergen influencing the development and expression of asthma in the studied population.ConclusionsPollen and cockroach were the most relevant allergen sources in the asthmatic population. The Salsola kali major allergen was the main cause for sensitization in the atopic patients suffering asthma. Using the Component Resolved Diagnosis, it was possible to identify a new Blattella germanica cockroach allergen (Blattella alpha amylase 53 kDa) that could sensitize a relevant percentage of this population.

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The most common aeroallergens in a tropical region in Southwestern Iran

Cited by 41 publications

References 32 publications

Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen

Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen

Prevalence and severity of sensitization reaction to food and inhalant allergens among allergic patients from Jeddah city: Saudi Arabia

Identification of the main allergen sensitizers in an Iran asthmatic population by molecular diagnosis

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