The Morphology of Three Previously Uncharacterized Human Respiratory Viruses that Grow in Organ Culture

Almeida, June D.; Tyrrell, D. A. J.

doi:10.1099/0022-1317-1-2-175

Cited by 234 publications

(160 citation statements)

References 7 publications

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“…Domermuth and Edwards (1957) examined ultrathin sections of IBV-infected chorio-allantoic membranes and observed intracytoplasmic particles having a doughnut-like appearance and measuring 178-200 nm in diameter. Viruses isolated from the respiratory tracts of persons with common colds have been described as " avian infectious bronchitislike " on account of their morphology in negatively stained preparations (Almeida and Tyrrell, 1967). However, differences in internal structure have been reported between the avian and human viruses present in ultrathin sections of infected chorio-allantoic membrane and human embryo kidney tissue culture cells respectively .…”

Section: Plates Lxv-lxviimentioning

confidence: 99%

An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus

Uppal

Chu

1970

Journal of Medical Microbiology

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PLATES LXV-LXVIIE A R L Y electron-microscope studies of metal-shadowed preparations of avian infectious bronchitis virus (IBV) revealed that the virus particle had a diameter of 60-120 nm (Reagan et al., 1948;Reagan and Brueckner, 1952). Later, negative staining showed it to differ from Newcastle disease virus in having less densely packed and more loosely attached surface projections that gave a strawberry-like appearance (Chu, 1964). Berry et al. (1964) confirmed that the projections of IBV are morphologically distinct from those of the myxoviruses, and reported that the diameter of the virus particle was 80-120 nm. Domermuth and Edwards (1957) examined ultrathin sections of IBV-infected chorio-allantoic membranes and observed intracytoplasmic particles having a doughnut-like appearance and measuring 178-200 nm in diameter. Viruses isolated from the respiratory tracts of persons with common colds have been described as " avian infectious bronchitislike " on account of their morphology in negatively stained preparations (Almeida and Tyrrell, 1967). However, differences in internal structure have been reported between the avian and human viruses present in ultrathin sections of infected chorio-allantoic membrane and human embryo kidney tissue culture cells respectively .

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Section: Plates Lxv-lxviimentioning

confidence: 99%

An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus

Uppal

Chu

1970

Journal of Medical Microbiology

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“…The morphology of the virus was determined using the negative staining procedure with pellets of concentrated virus obtained after ultracentrifugation of the supernatant medium of the infected kidney cells and Wi-38 [11]. Ultrathin sections of Vero and Wi-38 EBNV-infected cells were prepared and examined using standard methods [6].…”

Section: Methodsmentioning

confidence: 99%

Isolation of a Coronavirus from Kidney Biopsies of Endemic Balkan Nephropathy Patients

Uzelac-Keserović

Spasić

Bojanić

et al. 1999

Nephron

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Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33°C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis.

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“…This system provides a convenient method for determining the properties of some viruses which can only be cultivated with difficulty in monolayer cell cultures (Tyrrell & Bynoe, 1965;Almeida & Tyrrell, 1967). We previously established that rubella virus could be propagated in embryonic organ cultures derived from the upper respiratory tract (Best, Banatvala & Moore, 1968) and showed that virus could be detected electron microscopically in ultra-thin sections prepared from these cultures (Kistler, Best, Banatvala & T6ndury, 1967).…”

Section: Introductionmentioning

confidence: 99%

Further studies on the growth of rubella virus in human embryonic organ cultures: preliminary observations on interferon production in these cultures

Best

Banatvala

Smith

1971

J. Hyg.

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SUMMARYOrgan cultures prepared from 15 different organs obtained from 43 fetuses were consistently found to support the growth of rubella virus, irrespective of the gestational age of the fetus or the strain of rubella virus inoculated. Although rubella virus replicated in fetal lenses, adult lenses did not support the growth of rubella virus. Organs obtained from four fetuses between 8 and 17 weeks gestational age produced similar titres of an inhibitor which had the characteristics of interferon. The use of Trowell T8 medium and incubation in a mixture of 5 % CO2 in oxygen provided the most suitable conditions for the maintenance of most organ cultures. Under these circumstances it was possible to obtain adequate histological preparations from these organs, but light microscopy studies revealed no significant differences in sections of rubella inoculated and control organ cultures.

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The Morphology of Three Previously Uncharacterized Human Respiratory Viruses that Grow in Organ Culture

Cited by 234 publications

References 7 publications

An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus

An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus

Isolation of a Coronavirus from Kidney Biopsies of Endemic Balkan Nephropathy Patients

Further studies on the growth of rubella virus in human embryonic organ cultures: preliminary observations on interferon production in these cultures

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