The Morphological Effects of Asthma, As Well As Conventional and Alternative Asthma Therapies on Parietal and Chief Cells in the Stomach of BALB/c Mice

Vieira, Warren Antonio; Oberholzer, Hester Magdalena; Pretorius, Etheresia

doi:10.4067/s0717-95022011000400046

Cited by 2 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this reason, AQPs could be used as therapeutic targets in gastrointestinal disorders (Zhu et al, 2016). Altered secretion in gastric glands is known in some pathological conditions or pharmacological treatments (Driman et al, 1996; Toubia & Schubert, 2008; Vieira et al, 2011); anyway, the absence of AQP4 in KO mice does not significantly alter the secretion of gastric juice (Wang et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice

Mentino,

Nicchia,

Frigeri

et al. 2024

Microscopy Res & Technique

View full text Add to dashboard Cite

Aquaporins (AQPs) are important for water transport in the gastrointestinal tract. Changes in their expression and/or localization could cause in disorders and be used as therapeutic targets. Aquaporin‐4 (AQP4) is expressed predominantly on the basolateral membrane of the parietal cells in the corpus of the murine gastric glands. Although the secretion of gastric juice is not affected in AQP4‐deficient knockout, we evaluated by light microscopy whether the lack of AQP4 affects the glycopatterns of secreting gastric cells. Wild type (WT) and AQP4‐deficient knockout mice (KO) were fed a standard diet ad libitum before sacrifice. Segments of stomach corpus were collected, fixed in buffered formalin, and embedded in paraffin wax. Sections, 5‐μm thick, were analyzed by histochemical methods (Periodic acid‐Schiff, Alcian Blue pH 2.5), and binding of lectins specific to GalNAc (SBA, DBA), Gal (PNA) GlcNAc (WGA, GSAII) mannose and/or glucose (ConA), and fucose (UEA‐I, AAA, LTA). Immunohistochemical methods such as anti‐Muc6 for neck cells and anti‐ β‐ H+/K+‐ATPase for parietal cells were also performed. Compared to WT mice, in the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated, and fucosylated residues were observed; lower fucosylation resulted also in the parietal cells. The observed differences of KO in respect to WT could lead to severer pathological conditions.Research Highlights Glycopatterns in gastric glands were compared between wild type (WT) and AQP4‐deficient knockout (KO) mice by histochemical and lectin‐binding methods. In the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated and fucosylated residues were observed. In the parietal cells lower fucosylation also resulted. AQP4‐deficiency affects glycosylation and could result in altered functionality and pathological conditions.

show abstract