The morphological discrimination of microfilariae of Onchocerca volvulus from Mansonella ozzardi

Post, R.J.; Adams, Z. J. O.; Shelley, A. J.; Maia-Herzog, Marilza; Dias, A. P. A. Luna; Coscarón, Sixto

doi:10.1017/s003118200300324x

Cited by 34 publications

(29 citation statements)

References 16 publications

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“…The infection by M. ozzardi was determined by lightmicroscopy examination (200x and 400x) of Giemsastained thick smears of peripheral blood obtained by digital puncture with a sterile disposable lancet (Orihel & Eberhard 1982, Post et al 2003. The haemoscope survey took place between March 2006-August 2008.…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

Mansonella ozzardi in Amazonas, Brazil: prevalence and distribution in the municipality of Coari, in the middle Solimões River

Martins

Pessoa

Medeiros

et al. 2010

Mem. Inst. Oswaldo Cruz

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51 years old (26.9% urban and 61.5% rural). High prevalence rates were observed in retired people (27.1% urban area), housewives and farmer (41.6% and 25%, respectively, in rural area). The main clinical symptoms were joint pains and sensation of leg coldness. Only Cerqueirellum argentiscutum (Simuliidae) transmits M. ozzardi in this municipality (PIR = 5.6% urban and 7.1% rural). M. ozzardi is a widely distributed parasitic disease in Coari. Thus, temporary residency in the region of people from other localities involved with the local gas exploitation might be a contributing factor in spreading the disease.]]>

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Section: Patients Materials and Methodsmentioning

confidence: 99%

Mansonella ozzardi in Amazonas, Brazil: prevalence and distribution in the municipality of Coari, in the middle Solimões River

Martins

Pessoa

Medeiros

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…After air-drying, the blood was dehaemoglobinised in methylene blue, stained with Giemsa and microscopically examined (200X and 400X). When the blood smears were positive, the mf was identified following Post et al (2003). This study was approved by the Ethical Committee of the Instituto Nacional de Pesquisas da Amazônia (protocol n. 043/2005), Manaus, AM, Brazil.…”

Section: Methodsmentioning

confidence: 99%

Mansonella ozzardi in Brazil: prevalence of infection in riverine communities in the Purus region, in the state of Amazonas

Medeiros¹,

Py-Daniel²,

Barbosa³

et al. 2009

Mem. Inst. Oswaldo Cruz

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This study was undertaken to investigate the prevalence of Mansonella ozzardi infection and to estimate the parasitic infection rate (PIR) The filaria Mansonella ozzardi, one of the aetiological agents of mansonelliasis, is found on the American continent, as well as countries in the Caribbean. In South America, it has been reported in Panama, Colombia, Venezuela, Guyana, Suriname, Brazil, Argentina and Peru (Kozek et al. 1982). M. ozzardi is transmitted by Diptera insects of the families Ceratopogonidae in the Caribbean islands and Ceratopogonidae and Simuliidae in Central and South America (Shelley & Coscarón 2001).The pathogenicity of this filaria is not well defined. Several infected individuals show no symptoms; however, clinical manifestations that decrease the quality of life such as moderate fever, coldness in the legs, joint pains and headaches have been attributed to M. ozzardi infection (Batista et al. 1960b). Recently, the presence of ocular lesions has been reported as possibly associated with M. ozzardi infection (Branco et al. 1998, Cohen et al. 2008.The microfilaria M. ozzardi was detected in Brazil in the 1940s and 1950s (Deane 1949, Deane et al. 1954, Lacerda & Rachou 1956) and was described as a potentially extensive problem (Moraes 1958). Recently, some studies have shown that this filaria is still found with high prevalence (Medeiros et al. 2007(Medeiros et al. , 2008 and that simuliids are incriminated in the parasite's transmission in Brazil (Cerqueira 1959, Shelley & Shelley 1976, Shelley et al. 1980). Due to the absence of data and the importance of M. ozzardi for Amazonian populations, studies are still necessary to understand the geographical distribution of the cases, as well as the individual and cultural characteristics of the population in endemic areas that make them prone to infection. Indeed, eye lesions possibly associated with M. ozzardi have already been reported in the municipality of Pauini (Branco et al. 1998) and high prevalence rates were found in indigenous communities (Medeiros et al. 2007). Thus, our goal was to obtain data on human infection by M. ozzardi through comparison of prevalence rates between genders, as well as to examine the relationships between M. ozzardi infection and age and professional occupation. We also sought to identify the simuliid species involved in the transmission of M. ozzardi and to estimate the Parasitic Infection Rate (PIR) of the vectors. MATERIALs AND METHODsStudy area and population -This study was performed in the Purus and Pauini riverine communities in the municipality of Pauini (07º421'S 67º01'W), Amazonas (AM) (Fig. 1). The municipality of Pauini is located in southwest AM, about 900 km from Manaus. It has 17,092 inhabitants, with 6,970 in the urban zone and 10,122 in rural areas (IBGE 2000). The region is covered by native primary forest and presents long flood areas, accessible only by plane or boat. The rainy season is usually from November-March, with average annual temperatures between 24-26ºC, and a relative humidity bet...

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“…Two drops of blood (about 60µl) were collected from each individual and placed on a slide. After air-drying, the blood was dehemoglobinized, stained with Giemsa, placed under a microscope for identification of the species of microfilariae present 11 .…”

Section: Medeiros Jf Et Al -Mansonella Ozzardi Infection In Amazonasmentioning

confidence: 99%

Prevalence of Mansonella ozzardi among riverine communities in the municipality of Lábrea, State of Amazonas, Brazil

Medeiros

Py-Daniel

Barbosa

2011

Rev. Soc. Bras. Med. Trop.

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Introduction: Estimate the prevalence of Mansonella ozzardi infection and calculate the parasitic infection rate (PIR) in simuliid black flies in the municipality of Lábrea, State of Amazonas, Brazil. Methods: Prevalence was measured using the thick blood smear method collected from the fingers and was related to age, sex and occupation. Simuliidae were collected with a suction apparatus, then stained with hematoxylin and dissected to verify the PIR. Results:The average prevalence rate of M. ozzardi among the 694 individuals examined was 20.7%. Infection was higher in men (27.6%) than in women (14.3%) (p < 0.001) and occurred in most age groups, with the highest prevalence in the following age groups: 38-47 (40%), 48-57 (53.1%) and >58 (60.5%). The highest prevalence rates were observed in the retired (64%), followed by farm workers (47.1%). Infection by M. ozzardi was only identified in Cerqueirellum amazonicum (Simuliidae) with a PIR of 0.6%. Conclusions: This study showed a high prevalence of M. ozzardi in the riverine communities of Lábrea due to the lack of policies regarding the treatment of microfilaremic individuals in the region and an abundance of competent vectors for M. ozzardi. Keywords:Mansonelliasis. Mansonella ozzardi. Simuliidae. Municipality of Lábrea. Amazonas. RESUMOIntrodução: Estimar as prevalências de Mansonella ozzardi, e calcular a taxa de infecção parasitária nos simulídeos no município de Lábrea, Estado do Amazonas. Métodos: As prevalências foram obtidas através do método de gota espessa a partir do sangue coletado da polpa digital e foram relacionadas com a idade, sexo e ocupação. Os simulídeos foram coletados com capturador de sucção, posteriormente foram corados com hematoxilina e dissecados para se obter a taxa de infecção parasitária. Resultados: A prevalência de M. ozzardi entre as 694 pessoas examinadas foi de 20,7%. A infecção foi maior nos homens (27,6%) do que nas mulheres (14,3%) (p < 0,001) e ocorreu na maioria das faixas etárias com maiores prevalências nos indivíduos entre 38-47 (40%), 48-57 (53,1%) e >58 (60,5%) anos. As maiores prevalências foram observadas nos aposentados (64%) e agricultores (47,1%). Somente Cerqueirellum amazonicum (Simuliidae) foi encontrado infectado com M. ozzardi com uma taxa de infecção parasitária de 0,6%. Conclusões: Este trabalho mostra elevadas prevalências de M. ozzardi nas comunidades ribeirinhas de Lábrea devido à ausência de políticas de tratamento dos microfilarêmicos na região e a abundância de vetores competentes. Palavras-chaves:Mansonelose. Mansonella ozzardi. Simuliidae. Município de Lábrea. Amazonas.

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The morphological discrimination of microfilariae of Onchocerca volvulus from Mansonella ozzardi

Cited by 34 publications

References 16 publications

Mansonella ozzardi in Amazonas, Brazil: prevalence and distribution in the municipality of Coari, in the middle Solimões River

Mansonella ozzardi in Amazonas, Brazil: prevalence and distribution in the municipality of Coari, in the middle Solimões River

Mansonella ozzardi in Brazil: prevalence of infection in riverine communities in the Purus region, in the state of Amazonas

Prevalence of Mansonella ozzardi among riverine communities in the municipality of Lábrea, State of Amazonas, Brazil

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