The morphological and functional effects of exercise in the aquatic environment, performed before and/or after sciatic nerve compression in Wistar rats

Kakihata, Camila Mayumi Martin; Malanotte, Jéssica Aline; Karvat, Jhenifer; Brancalhão, Rose Meire Costa; Ribeiro, Lucinéia de Fátima Chasko; Bertolini, Gladson Ricardo Flôr

doi:10.12965/jer.1632670.335

Cited by 10 publications

(7 citation statements)

References 23 publications

(27 reference statements)

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“…No difference was found for luminal capillaries area. The angiogenic action of exercise is known ( Kakihata et al, 2016 ; Lansford et al, 2016 ). Similar to that found in this study, Kakihata et al (2016) reported a larger number of endoneurial blood vessels in sciatic nerve crushed that went through an exercise protocol of aquatic jumps.…”

Section: Discussionmentioning

confidence: 99%

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Moura¹,

Morais²,

Barbosa³

et al. 2022

J Exerc Rehabil

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Peripheral nerve injuries are cause of sensory disturbances and in functional abilities, and are associated personal and social costs. Strat-egies that maximize nerve regeneration and functional recovery are necessary, the exercise is an option. This study evaluated the effects of forced swimming exercise on neuromuscular histomorphometry and on functional recovery in a median nerve crush model. Sixteen Wistar rats underwent median nerve crush and were divided into control group (CG) and swimming group (SG). The forced swimming protocol started one week after the injury and was performed for 1 hr a day, 5 days per week, for 2 weeks. The rats swam with an overload of 5% and 10% of body weight in the first and second week, respectively. The functional recovery was assessed in three moments using the grasping test. On day 21, fragments of the median nerve and of the forearm flexors mus-cles were removed for histomorphometric analysis. The SG had func-tional recovery impaired (p<0.001) and presented lower myelinated fi-bers number, fiber and axon minimal diameter, myelin thickness and g-ratio in the proximal e distal segments of the median nerve (p<0.005) and area muscle fiber (p<0.005) than CG. Also, the SG presented a number of capillaries in the proximal segments of the median nerve greater than CG (p<0.005). The exercise protocol used in this study im-paired the regeneration of the median nerve and negatively influenced the functional recovery.

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Section: Discussionmentioning

confidence: 99%

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Moura¹,

Morais²,

Barbosa³

et al. 2022

J Exerc Rehabil

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show abstract

“…The angiogenic action of exercise is known (Lansford et al, 2016; Terra et al, 2012); a higher number of vessels could minimize the effects of hypoxia and enhance nerve regeneration (Kakihata et al, 2016). In this study, no difference in the number of endoneural vessels was observed among groups, in contrast to what was found in a sciatic nerve crush model, in which exercise promoted increase in endoneural vessels (Kakihata et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Effects of two intensities of treadmill exercise on neuromuscular recovery after median nerve crush injury in Wistar rats

et al. 2019

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Considering the potential action of exercise on neuroplasticity and the need to adapt protocols to enhance functional recovery after nerve injury, this study evaluated the effects of two intensities of treadmill exercise on nervous and muscular tissues and functional recovery after nerve crush injury. Wistar rats were distributed into sedentary group (SED), and 10 m/min (EG10) and 17 m/min (EG17) exercise groups. The exercise started one week after the injury. Ten daily sessions were performed with a 2-day interval after the fifth day. The flexor digitorum muscle and two segments of the median nerve were analysed histomorphometrically by light microscopy and computer analysis. Function was evaluated by grasping test, in 3 moments. Approval number: 016/2013. In the proximal segments of the median nerve, the diameter of myelinated fibres and axon, the myelin sheath thickness and the ratio of axon diameter to fibre diameter (g ratio) were significantly larger ( P <0.05) in the EG10. The number of myelinated fibres was lesser in the EG17 than the other groups ( P <0.05). No difference in the number of myelinated fibres among groups was observed in the distal segments, but the SED presented significantly larger axon and fibre diameters than those that performed exercise. The EG10 presented greater area and diameter of muscle fibres ( P <0.05) and functional improvement observed on the 21st day after injury ( P <0.05) compared with the EG17 and SED. Continuous exercise at 10 m/min accentuates nerve regeneration, accelerating functional recovery and preventing muscle atrophy.

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“…These SFI parameters were applied by Bain et al [18]. SFI index was according to a previously described method [19]. Interpolation of identical values of IT, PL, and TS from the right and left hind feet results in an SFI value close to 0 in normal rats, whereas an SFI value of –100 represents impairment.…”

Section: Methodsmentioning

confidence: 99%

Scolopendra subspinipes mutilans Extract Suppresses Inflammatory and Neuropathic Pain In Vitro and In Vivo

Hwang

Jin

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Background Sciatic nerve injury develops from a variety of pathological causes, including traumatic injury and neuroinflammatory disorders, which are accompanied by pathological changes that have a critical impact on neuropathic pain and locomotor activity. Extracts of Scolopendra subspinipes mutilans (SSM) are used in traditional medicine for the treatment of a wide range of neuropathic diseases, including lower back pain, peripheral neuropathy, and sciatic nerve injury. Although SSM shows anti-inflammatory, antibacterial, and anticonvulsant activities, its diverse mechanisms of action remain unclear. Thus, the present study examined the effects of SSM in vitro and in vivo. Methods To estimate the anti-inflammatory effects of SSM, inflammatory conditions were induced using lipopolysaccharide (LPS) in RAW 264.7 cells, and inflammatory-related factors were evaluated by enzyme-linked immunosorbent assay (ELISA) and western blotting analyses. Sciatic nerve crush injury (SNCI) was induced in rats using a surgical clip instrument. The effects of SSM in the SNCI model were evaluated in behavioral tests by calculating the sciatic functional index (SFI) and measuring thermal hyperalgesia sensitivity and by monitoring inflammatory factors expression in western blotting analyses. Results We observed the anti-inflammatory effects of SSM treatment both in vitro and in vivo. The PGE2 and NO production were suppressed by SSM. Protein analyses indicated that expression of NF-κB and degradation of IκBα were suppressed by SSM treatment. In addition, the levels of iNOS, TNF-α, IL-6, and COX-2 expression were reduced by SSM treatment in RAW 264.7 cells and in the SNCI-induced animals. In behavioral studies, SSM treatment enhanced the SFI and improved the thermal sensitivity test results. Conclusions Our results suggest that SSM suppresses the production of inflammatory factors via the NF-κB pathway and accelerates the morphological and functional recovery of the peripheral nervous system. Hence, SSM may be a useful therapeutic candidate for treatment of neuropathic pain diseases.

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The morphological and functional effects of exercise in the aquatic environment, performed before and/or after sciatic nerve compression in Wistar rats

Cited by 10 publications

References 23 publications

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Effects of two intensities of treadmill exercise on neuromuscular recovery after median nerve crush injury in Wistar rats

Scolopendra subspinipes mutilans Extract Suppresses Inflammatory and Neuropathic Pain In Vitro and In Vivo

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