The morphogenesis of cell hairs on Drosophila wings

Mitchell, Herschel K.; Roach, Joan L. M.; Petersen, Nancy S.

doi:10.1016/0012-1606(83)90040-4

Cited by 63 publications

(57 citation statements)

References 21 publications

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“…center of the wing cell at ‫35-74ف‬ hr a.p.f. (Mitchell et al 1983). We have found that frtz mutant prehairs Similar cell autonomy is shown by the other mutations in other PCP effector genes (Gubb and Garcia-Bellido form at the same time as wild type but initiate at multiple alternative sites at the apical periphery of the pupal 1982; Adler et al 1994; Collier and Gubb 1997) although weak nonautonomy has described for in clones wing cell ( Figure 3A).…”

Section: Methodssupporting

confidence: 65%

The WD40 Repeat Protein Fritz Links Cytoskeletal Planar Polarity to Frizzled Subcellular Localization in the Drosophila Epidermis

et al. 2005

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Much of our understanding of the genetic mechanisms that control planar cell polarity (PCP) in epithelia has derived from studies of the formation of polarized cell hairs during Drosophila wing development. The correct localization of an F-actin prehair to the distal vertex of the pupal wing cell has been shown to be dependent upon the polarized subcellular localization of Frizzled and other core PCP proteins. However, the core PCP proteins do not organize actin cytoskeletal polarity directly but require PCP effector proteins such as Fuzzy and Inturned to mediate this process. Here we describe the characterization of a new PCP effector gene, fritz, that encodes a novel but evolutionarily conserved coiled-coil WD40 protein. We show that the fritz gene product functions cell-autonomously downstream of the core PCP proteins to regulate both the location and the number of wing cell prehair initiation sites.

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Section: Methodssupporting

confidence: 65%

The WD40 Repeat Protein Fritz Links Cytoskeletal Planar Polarity to Frizzled Subcellular Localization in the Drosophila Epidermis

et al. 2005

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“…Yellow synthesis begins around the time of cuticle secretion (Mitchell et al, 1983;Walter et al, 1991). In our hands, procuticle was partially formed at 54 hours apf (29°C) and reached its final thickness by 63 hours apf (29°C) (Fig.…”

Section: Mgl Controls Cuticle Pigmentation By Reducing Yellow Proteinmentioning

confidence: 51%

“…The organization of cuticle in distinct layers reflects the ordered synthesis and secretion of different cuticular components by ectodermal epithelial cells (Doctor et al, 1985;Fristrom et al, 1986;Locke, 2001;Mitchell et al, 1983;Moussian, 2010). However, the different layers also continue to develop and change their appearance after secretion as the cuticle matures (Moussian et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Megalin-dependent Yellow endocytosis restricts melanization in theDrosophilacuticle

2011

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SUMMARYThe cuticular exoskeleton of arthropods is a composite material comprising well-separated layers that differ in function and molecular constituents. Epidermal cells secrete these layers sequentially, synthesizing components of distal cuticle layers before proximal ones. Could the order of synthesis and secretion be sufficient to account for the precision with which cuticle components localize to specific layers? We addressed this question by studying the spatial restriction of melanization in the Drosophila wing. Melanin formation is confined to a narrow layer within the distal procuticle. Surprisingly, this tight localization depends on the multi-ligand endocytic receptor Megalin (Mgl). Mgl acts, in part, by promoting endocytic clearance of Yellow. Yellow is required for black melanin formation, and its synthesis begins as cuticle is secreted. Near the end of cuticle secretion, its levels drop precipitously by a mechanism that depends on Mgl and Rab5-dependent endocytosis. In the absence of Mgl, Yellow protein persists at higher levels and melanin granules form ectopically in more proximal layers of the procuticle. We propose that the tight localization of the melanin synthesis machinery to the distal procuticle depends not only on the timing of its synthesis and secretion, but also on the rapid clearance of these components before synthesis of subsequent cuticle layers.

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“…As described above, dsh pupae exhibit defects similar to those of wg; in addition, they have a tissue polarity defect in which the polarity of hairs and bristles is chaotic, seen most clearly on the dorsal thorax. Because a given hair is the product of a single epidermal cell (Poodry 1980;Mitchell et al 1983), this feature can be used to determine dsh phenotype at the level of individual cells. Thoracic dsh clones show the tissue polarity defect, but adjacent wildtype cells have hairs of wild-type polarity (Fig.…”

Section: Genes and Developmentmentioning

confidence: 99%

The Drosophila segment polarity gene dishevelled encodes a novel protein required for response to the wingless signal.

Klingensmith

Nusse²,

Perrimon³

1994

Genes Dev.

369

209

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The morphogenesis of cell hairs on Drosophila wings

Cited by 63 publications

References 21 publications

The WD40 Repeat Protein Fritz Links Cytoskeletal Planar Polarity to Frizzled Subcellular Localization in the Drosophila Epidermis

The WD40 Repeat Protein Fritz Links Cytoskeletal Planar Polarity to Frizzled Subcellular Localization in the Drosophila Epidermis

Megalin-dependent Yellow endocytosis restricts melanization in theDrosophilacuticle

The Drosophila segment polarity gene dishevelled encodes a novel protein required for response to the wingless signal.

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