The monoclonal antibody JIM19 modulates abscisic acid action in barley aleurone protoplasts

Wang, M.; Heimovaara-Dijkstra, S.; Meulen, R.M. van der; Knox, John; Neill, Steven J.

doi:10.1007/bf00201384

Cited by 17 publications

(15 citation statements)

References 29 publications

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“…There are several indications that cell wall molecules can influence ABA signalling and sensitivity to exogenous ABA, including an extensin epitope (Wang et al. , 1995), an arabinogalactan‐protein (van Hengel & Roberts, 2003) and a putative family 64 glycosyltransferase (Bown et al.…”

Section: Discussionmentioning

confidence: 99%

ABA signalling modulates the detection of the LM6 arabinan cell wall epitope at the surface of Arabidopsis thaliana seedling root apices

2011

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Summary• The hormonal and physiological regulations underpinning the cell contexts of structural features of the heterogeneous cell wall pectic polysaccharide rhamnogalacturonan-I are far from being understood.• The effect of the modulation of abscisic acid (ABA) concentrations and sensitivity on the detection of the LM6 1,5-arabinan epitope at the surface of Arabidopsis thaliana seedling root apices was assessed by means of fluorescence imaging.• Treatment with 50 nM ABA resulted in an increase in the detection of the LM6 epitope at the root surface in the region of the meristem. An inhibitor of ABA biosynthesis and introduction of the ABA synthesis mutation aba3-2 resulted in reduced epitope detection. The same ABA application resulted in an increase in the number of epidermal root meristem cells and both this and LM6 epitope detection were specifically disrupted in the abi4 ABA-insensitive mutant. These two effects were uncoupled with the application of higher ABA concentrations, which resulted in a reduction in the number of epidermal root meristem cells but increased LM6 epitope detection.• This work demonstrates a role for ABI4-mediated ABA signalling in the modulation of pectic arabinan occurrence at the A. thaliana root meristem.

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Section: Discussionmentioning

confidence: 99%

ABA signalling modulates the detection of the LM6 arabinan cell wall epitope at the surface of Arabidopsis thaliana seedling root apices

2011

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“…Additional indirect in vitro evidence for an ABA receptor complex at the cell surface was provided by surface plasmon resonance studies in conjunction with a protoplast ABA-inducible gene expression assay (Desikan et al, 1999). JIM19 is one of a panel of monoclonal antibodies generated against pea guard cell protoplasts that recognizes a cell surface glycoprotein and can modulate ABA responses in barley aleurone and rice protoplasts (Wang et al, 1995;Desikan et al, 1999). Desikan et al (1999) observed specific binding of plasma membranes to JIM19 that was antagonized significantly by ABA but not by the biologically inactive ABA catabolite phaseic acid.…”

Section: Aba Perception Recognition Site(s)mentioning

confidence: 99%

Abscisic Acid Biosynthesis and Response

Finkelstein¹,

Rock

2002

The Arabidopsis Book

190

141

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“…Using surface plasmon resonance in conjunction with flow cytometry, Desikan et al (60) provided indirect evidence for a putative ABA-receptor complex that interacts with a cell-surface glycoprotein. A monoclonal antibody (JIM19) generated against pea guard cell protoplasts specifically binds to plasma membrane glycoproteins and antagonizes ABA-inducible gene expression in rice and barley (60,76). It is noteworthy in this context that PLC is involved in shedding of arabinogalactan proteins by plasma membrane vesicles (77).…”

Section: La 3ϩ and Pld Interaction With Aba Effectors Vp1 And Abi1mentioning

confidence: 99%

Functional Interactions of Lanthanum and Phospholipase D with the Abscisic Acid Signaling Effectors VP1 and ABI1-1 in Rice Protoplasts

Gampala

Hagenbeek

Rock

2001

Journal of Biological Chemistry

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cis,trans-Abscisic acid (ABA) plays an important role in plant growth and development, regulation of seed maturation, germination, and adaptation to environmental stresses. Knowledge of ABA mechanisms of action and the interactions of components required for ABA signal transduction is far from complete. Using transient gene expression in rice protoplasts, we observed additive and inhibitory effects between maize VP1 (Viviparous-1, a transcriptional activator) and a dominant-negative mutant protein phosphatase, ABI1-1 (ABA-insensitive-1-1), from Arabidopsis. Lanthanide ions were shown to be specific agonists of ABA-inducible gene expression and to interact synergistically with ABA and overexpressed VP1. Both VP1 and lanthanum activities could be antagonized by coexpression of ABI1-1, which demonstrates the specific ABA dependence of these effectors on ABA-regulated gene expression. We obtained pharmacological evidence that phospholipase D (PLD) functions in ABA-inducible gene expression in rice. Antagonism of ABA, VP1, and lanthanum synergy by 1-butanol, a specific inhibitor of PLD, was similar to the inhibition by coexpression of ABI1-1. These results demonstrate that ABA, VP1, lanthanum, PLD, and ABI1 are all involved in ABA-regulated gene expression and are consistent with an integrated model whereby La 3؉ acts upstream of PLD.cis,trans-Abscisic acid (ABA) 1 modulates seed development, dormancy, cell division, stomatal movements, and cellular responses to environmental stresses such as drought, cold, salt, pathogen attack, and UV light (1-3). Despite its importance in plant growth and development, the mechanisms of ABA action are largely unknown, and there may be more than one ABA signal transduction pathway leading to both fast (guard cell ion fluxes) and slow (gene regulation) cellular responses (4 -6). Transient gene expression studies with LEA (late embryogenesis abundant) and drought-inducible gene promoters have defined the cis-acting elements necessary and sufficient to confer ABA-inducible transcription (7-10). Separate ABA-responsive elements and coupling elements function cooperatively and redundantly as an ABA response complex (9,11,12).Recent insight into the mechanisms of ABA-inducible transcription have come from cloning of TRAB1 (transcription factor responsible for ABA regulation), a basic leucine zipper transcription factor that binds ABA response complex promoter elements and VP1 (Viviparous-1), a transcriptional activator required for ABA-regulated gene expression during seed maturation (13-15). Other proteins interact with ABA response complexes, VP1 orthologs, and basic leucine zipper factors, but there is no direct evidence that these factors function in ABA signaling (16 -27). Regulation by ABA of TRAB1 and VP1 transactivation of ABA response complexes is not at the level of DNA binding, based on transient gene expression and in vivo footprinting assays (13,28). Consistent with this model is the finding that the bean ortholog of VP1, PvALF, functions to remodel chromatin independent of e...

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The monoclonal antibody JIM19 modulates abscisic acid action in barley aleurone protoplasts

Cited by 17 publications

References 29 publications

ABA signalling modulates the detection of the LM6 arabinan cell wall epitope at the surface of Arabidopsis thaliana seedling root apices

ABA signalling modulates the detection of the LM6 arabinan cell wall epitope at the surface of Arabidopsis thaliana seedling root apices

Abscisic Acid Biosynthesis and Response

Functional Interactions of Lanthanum and Phospholipase D with the Abscisic Acid Signaling Effectors VP1 and ABI1-1 in Rice Protoplasts

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