The Monoclonal Antibody against the Major Diagnostic Antigen of<i>Paracoccidioides brasiliensis</i>Mediates Immune Protection in Infected BALB/c Mice Challenged Intratracheally with the Fungus

Buissa-Filho, R.; Puccia, Rosana; Marques, Alexandre F.; Costa-Pinto, Frederico Azevedo da; Muñoz, Julián E.; Nosanchuk, Joshua D.; Travassos, Luiz R.; Taborda, Carlos Pelleschi

doi:10.1128/iai.00349-08

Cited by 66 publications

(51 citation statements)

References 53 publications

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“…The count of fungal cell viability, expressed as colony forming units (CFU), was used to determine the survival rate of melanized and nonmelanized yeast when exposed to oxidants. Intratracheal infection of BALB/c mice with 3x10 5 yeast cells of P. brasiliensis, melanized and nonmelanized, was performed as previously described (Buissa-filho et al 2008). For statistical analysis, the GraphPad Prism5 software was utilized.…”

Section: Methodsmentioning

confidence: 99%

Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18

Silva

Thomaz

Marques

et al. 2009

Mem. Inst. Oswaldo Cruz

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Section: Methodsmentioning

confidence: 99%

Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18

Silva

Thomaz

Marques

et al. 2009

Mem. Inst. Oswaldo Cruz

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“…In patients that underwent chemo-therapy, both gp43 and gp70 are markers for monitoring successive treatment and cure through their decreased antigenemia and specific antibody response (Marques da Silva et al 2004. In the experimental Balb/c model of PCM infection, anti-gp43 mAb 3E effectively reduced the fungal burden and promoted phagocytosis in vitro (Buissa-Filho et al 2008). The recognized epitope in the gp43 was mapped to the sequence NHVRIPIGYWAV shared with Aspergillus fumigatus, A. oryzae and B. graminis internal sequences of β-1,3-glucanases.…”

Section: A Peptide Vaccine Against Paracoccidioidomycosismentioning

confidence: 99%

Antifungal and antitumor models of bioactive protective peptides

Rodrigues

Dobroff

Taborda

et al. 2009

An. Acad. Bras. Ciênc.

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Peptides are remarkably reactive molecules produced by a great variety of species and able to display a number of functions in uni-and multicellular organisms as mediators, agonists and regulating substances. Some of them exert cytotoxic effects on cells other than those that produced them, and may have a role in controlling subpopulations and protecting certain species or cell types. Presently, we focus on antifungal and antitumor peptides and discuss a few models in which specific sequences and structures exerted direct inhibitory effects or stimulated a protective immune response. The killer peptide, deduced from an antiidiotypic antibody, with several antimicrobial activities and other Ig-derived peptides with cytotoxic activities including antitumor effects, are models studied in vitro and in vivo. Peptide 10 from gp43 of P. brasiliensis (P10) and the vaccine perspective against paracoccidioidomycosis is another topic illustrating the protective effect in vivo against a pathogenic fungus. The cationic antimicrobial peptides with antitumor activities are mostly reviewed here. Local treatment of murine melanoma by the peptide gomesin is another model studied at the Experimental Oncology Unit of UNIFESP.

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“…IgG2a and IgG2b MAbs to gp43 of P. brasiliensis were shown to reduce fungal burden and was associated with the enhanced phagocytosis of P. brasiliensis by macrophages leading to increased nitric oxide production [82]. Prophylaxis passive intranasal administration of anti-glycoprotein A IgM or IgG1 switch variant MAb protected against murine P. carinii [83].…”

Section: Demonstration Of Protective Role Of Antibodies In Fungal Infmentioning

confidence: 99%

The protective role of immunoglobulins in fungal infections and inflammation

2014

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International audienceIncreased incidence of fungal infections in the immunocompromised individuals and fungi-mediated allergy and inflammatory conditions in immunocompetent individuals is a cause of concern. Consequently, there is a need for efficient therapeutic alternatives to treat fungal infections and inflammation. Several studies have demonstrated that antibodies or immunoglobulins have a role in restricting the fungal burden and their clearance. However, based on the data from monoclonal antibodies, it is now evident that the efficacy of antibodies in fungal infections is dependent on epitope specificity, abundance of protective antibodies, and their isotype. Antibodies confer protection against fungal infections by multiple mechanisms that include direct neutralization of fungi and their antigens, inhibition of growth of fungi, modification of gene expression, signaling and lipid metabolism, causing iron starvation, inhibition of polysaccharide release, and biofilm formation. Antibodies promote opsonization of fungi and their phagocytosis, complement activation, and antibody-dependent cell toxicity. Passive administration of specific protective monoclonal antibodies could also prove to be beneficial in drug resistance cases, to reduce the dosage and associated toxic symptoms of anti-fungal drugs. The longer half-life of the antibodies and flexibilities to modify their structure/forms are additional advantages. The clinical data obtained with two monoclonal antibodies should incite interests in translating pre-clinical success into the clinics. The anti-inflammatory and immunoregulatory role of antibodies in fungal inflammation could be exploited by intravenous immunoglobulin or IVIg

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The Monoclonal Antibody against the Major Diagnostic Antigen ofParacoccidioides brasiliensisMediates Immune Protection in Infected BALB/c Mice Challenged Intratracheally with the Fungus

Cited by 66 publications

References 53 publications

Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18

Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18

Antifungal and antitumor models of bioactive protective peptides

The protective role of immunoglobulins in fungal infections and inflammation

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