The molecular prevalence and MSA-2b gene-based genetic diversity of Babesia bovis in dairy cattle in Thailand

Simking, P.; Saengow, S.; Bangphoomi, Kunan; Sarataphan, Nopporn; Wongnarkpet, Sirichai; Inpankaew, Tawin; Jittapalapong, Sathaporn; Munkhjargal, Tserendorj; Sivakumar, Thillaiampalam; Yokoyama, Naoki; Igarashi, Ikuo

doi:10.1016/j.vetpar.2013.07.015

Cited by 22 publications

(18 citation statements)

References 24 publications

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“…As no fever like symptoms were reported in this cow who spent her life at a USDA research facility near Miles City, Montana and babesiosis has been reported to have been eradicated in the United States with vaccination no longer being required 20 , we suspect that Dominette was asymptomatically infected with a non-pathogenic strain of Babesia spp., as has been previously reported in Turkey 21 , Syria 22 , and Thailand 23 . Although it is currently not possible to determine the exact species of parasite, we can assess the animal's parasite burden via deep sequence data by evaluating the number of species to which the contigs of unmapped reads align and the number of contigs that align to each species.…”

Section: Discussionsupporting

confidence: 88%

What’s in your next-generation sequence data? An exploration of unmapped DNA and RNA sequence reads from the bovine reference individual

Whitacre

Tizioto

Kim

et al. 2015

Preprint

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Next-generation sequencing projects commonly commence by aligning reads to a reference genome assembly. While improvements in alignment algorithms and computational hardware have greatly enhanced the efficiency and accuracy of alignments, a significant percentage of reads often remain unmapped. We generated de novo assemblies of unmapped reads from the DNA and RNA sequencing of the Bos taurus reference individual and identified the closest matching sequence to each contig by alignment to the NCBI non-redundant nucleotide database using BLAST. As expected, many of these contigs represent vertebrate sequence that is absent, incomplete, or misassembled in the UMD3.1 reference assembly. However, numerous additional contigs represent invertebrate species. Most prominent were several species of Spirurid nematodes and a blood-borne parasite, Babesia bigemina. These species are not known to infect taurine cattle and the reference animal appears to have been host to unsequenced sister species. We demonstrate the importance of exploring unmapped reads to ascertain sequences that are either absent or misassembled in the reference assembly and for detecting sequences indicative of infectious or symbiotic organisms.

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Section: Discussionsupporting

confidence: 88%

What’s in your next-generation sequence data? An exploration of unmapped DNA and RNA sequence reads from the bovine reference individual

Whitacre

Tizioto

Kim

et al. 2015

Preprint

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“…The genetic diversity of B. bovis msa-2 genes was previously shown among various populations (Berens et al, 2005;Leroith et al, 2005;Altangeral et al, 2012;Simking et al, 2013;Sivakumar et al, 2013;Tattiyapong et al, 2014). The antigenic variation of B. bovis enables the parasites to avoid the immune response of cattle by continual switching of surface proteins -a mechanism used by the pathogen to establish chronic infections within the host (Allred et al, 2000;Carcy et al, 2006;Dzikowski and Kirk, 2006).…”

Section: Discussionmentioning

confidence: 98%

“…Similarly, B. bovis polyclonal antibodies specific for MSA2a1/MSA-2a2, MSA-2b, and MSA-2c inhibited the invasion of erythrocytes by merozoites in vitro (Wilkowsky et al, 2003). Several studies utilized the variable merozoite surface antigens of B. bovis as a genetic marker to show the existence of variant strains in different geographic regions around the world (Berens et al, 2005;Leroith et al, 2005;Simking et al, 2013;Sivakumar et al, 2013;Tattiyapong et al, 2014). Blood-derived live attenuated B. bovis vaccines are currently used to control babesiosis in Australia, South Africa, Argentina, Brazil, and Israel (Pipano et al, 2002;Shkap et al, 2005Shkap et al, , 2007Gohil et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of Babesia bovis merozoite surface antigens-2 (MSA-2) isolates from bovine blood and Rhipicephalus annulatus ticks in Israel

Molad¹,

Fleiderovitz²,

Leibovich³

et al. 2014

Veterinary Parasitology

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“…Studies conducted in Australia (Berens et al, 2005;Leroith et al, 2005), Sri Lanka (Sivakumar et al, 2013b), the Philippines (Tattiyapong et al, 2014), Thailand (Simking et al, 2013), Israel (Molad et al, 2014), Brazil (Nagano et al, 2013), and Mexico Genis et al, 2008Genis et al, , 2009) have found that B. bovis isolates in these countries are genetically diverse, according to their MSA gene sequences.…”

Section: Introductionmentioning

confidence: 99%

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Yokoyama

Sivakumar

Tuvshintulga

et al. 2015

Infection, Genetics and Evolution

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The genes that encode merozoite surface antigens (MSAs) in Babesia bovis are genetically diverse. In this study, we analyzed the genetic diversity of B. bovis MSA-1, MSA-2b, and MSA-2c genes in Vietnamese cattle and water buffaloes. Blood DNA samples from 258 cattle and 49 water buffaloes reared in the Thua Thien Hue province of Vietnam were screened with a B. bovis-specific diagnostic PCR assay. The B. bovis-positive DNA samples (23 cattle and 16 water buffaloes) were then subjected to PCR assays to amplify the MSA-1, MSA-2b, and MSA-2c genes. Sequencing analyses showed that the Vietnamese MSA-1 and MSA-2b sequences are genetically diverse, whereas MSA-2c is relatively conserved. The nucleotide identity values for these MSA gene sequences were similar in the cattle and water buffaloes. Consistent with the sequencing data, the Vietnamese MSA-1 and MSA-2b sequences were dispersed across several clades in the corresponding phylogenetic trees, whereas the MSA-2c sequences occurred in a single clade. Cattle- and water-buffalo-derived sequences also often clustered together on the phylogenetic trees. The Vietnamese MSA-1, MSA-2b, and MSA-2c sequences were then screened for recombination with automated methods. Of the seven recombination events detected, five and two were associated with the MSA-2b and MSA-2c recombinant sequences, respectively, whereas no MSA-1 recombinants were detected among the sequences analyzed. Recombination between the sequences derived from cattle and water buffaloes was very common, and the resultant recombinant sequences were found in both host animals. These data indicate that the genetic diversity of the MSA sequences does not differ between cattle and water buffaloes in Vietnam. They also suggest that recombination between the B. bovis MSA sequences in both cattle and water buffaloes might contribute to the genetic variation in these genes in Vietnam.

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The molecular prevalence and MSA-2b gene-based genetic diversity of Babesia bovis in dairy cattle in Thailand

Cited by 22 publications

References 24 publications

What’s in your next-generation sequence data? An exploration of unmapped DNA and RNA sequence reads from the bovine reference individual

What’s in your next-generation sequence data? An exploration of unmapped DNA and RNA sequence reads from the bovine reference individual

Genetic polymorphism of Babesia bovis merozoite surface antigens-2 (MSA-2) isolates from bovine blood and Rhipicephalus annulatus ticks in Israel

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

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