The molecular mechanisms of signal pathway activating effect of E2F-1/NF-κB/GSK-3β on cognitive dysfunction of Alzheimer rats

Abstract

Alzheimer disease (AD) seriously harms human health and its onset is insidious. Therefore, it is of great significance to find out the pathogenesis of AD disease for improving the prevention and treatment effect of the disease. The study drew attention to the influence of E2F-1/NF-κB/GSK-3β signaling pathway on cognitive dysfunction of Alzheimer rats. 60 specific pathogen-free (SPF) SD rats were selected as research subjects. The, the AD model was created by injecting Aβ1-42 into hippocampus CA1 region of AD rats using a microscopic syringe. Besides, Morris water maze test and Western blot were performed to detect the cognitive function, the levels of destination protein and active oxidation products in the brain of rats. Compared to the Sham group, the escape latency and the distance of the model group significantly increased ( P < 0.05), and the number of times to pass the target quadrant was significantly reduced ( P < 0.05); the expression levels of E2F-1 and NF-κB protein in the hippocampus and the phosphorylation levels of Tau231, Tau262, Tau396, Tau404 and T216-GSK-3β protein of the model group were significantly increased ( P < 0.05); the ROS/RNS value in the hippocampus of the model group significantly increased ( P < 0.05). AD model rats exhibit obvious cognitive dysfunction, which is associated with the activation of E2F-1/NF-κB/GSK-3β signaling pathway and the heightened Tau protein phosphorylation level.

Effects of Plant-Derived Glycerol Monolaurate (GML) Additive on the Antioxidant Capacity, Anti-Inflammatory Ability, Muscle Nutritional Value, and Intestinal Flora of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)

“…In recent years, the production of grouper culture has been rising, and the scale of culture has been expanding. However, the rapid development of the intensive culture of grouper may induce oxidative stress in fish, caused by aquatic animals, and affect the antioxidant protection capacity of aquatic animals [1].…”

Interaction of Genetics, Amyloid-beta and Inflammation as Mediators of Brain Structure Change in Cognitive Decline

“…Rs10206871, mapped to the enhancer of SERTAD2, was found to have shared genetic effects on both EGF and inferior lateral ventricles. SERTAD2 could enhance E2F transcription factor activity to control both in ammatory and neuronal cell cycling pathways [37]. We also identi ed SNPs that in uence WMH changes over the 12-month follow-up period, as well as CD40L and EGF.…”

Functional Implications of Protein Arginine Methyltransferases (PRMTs) in Neurodegenerative Diseases

“…Induction of Aβ in primary cortical neurons leads to a 54% drop in PRMT5 expression and a 70% increase in E2F-1 expression. Studies have revealed that the protein Tau in the brain tissues of AD patients has detectable phosphorylation changes at Thr231, Ser235, Ser396, Tyr394, Ser404, and Thr181 [ 53 ], suggesting that the protein is intimately linked to neurodegeneration. Further, activation of GSK-3β results in the phosphorylation of Tau at the Ser404 location and leads to the development of AD [ 54 ].…”