The molecular mechanism of the anticancer effect of Artonin E in MDA-MB 231 triple negative breast cancer cells

Etti, Imaobong Christopher; Abdullah, Rasedee; Kadir, Arifah Abdul; Hashim, Najihah Mohd; Yeap, Swee Keong; Imam, Mustapha Umar; Ramli, Faiqah; Malami, Ibrahim; Lam, K. L.; Etti, Ubong; Waziri, Peter; Rahman, Marsitoh

doi:10.1371/journal.pone.0182357

Cited by 42 publications

(29 citation statements)

References 59 publications

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“…In general, these results are in good agreement with the findings already documented in the scientific literature that SKBR3 is a HER2-overexpressing cell line [ 33 ], MCF-7 and T47D are low HER2-overexpressing cell lines [ 34 ] and MDA-MB-231 is considered triple negative (no expression of HER2, progesterone and estrogen receptors) [ 35 ]. Russo et al reported that HER2 expressed at T47D is predominantly cytosolic [ 36 ].…”

Section: Resultssupporting

confidence: 90%

Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering

et al. 2018

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Biosensor technology has great potential for the detection of cancer through tumor-associated molecular biomarkers. In this work, we describe the immobilization of the recombinant humanized anti-HER2 monoclonal antibody (trastuzumab) on a silver nanostructured plate made by pulsed laser deposition (PLD), over a thin film of Au(111). Immobilization was performed via 4-mercapto benzoic acid self-assembled monolayers (4-MBA SAMs) that were activated with coupling reagents. A combination of immunofluorescence images and z-stack analysis by confocal laser scanning microscopy (CLSM) allowed us to detect HER2 presence and distribution in the cell membranes. Four different HER2-expressing breast cancer cell lines (SKBR3 +++, MCF-7 +/−, T47D +/−, MDA-MB-231 −) were incubated during 24 h on functionalized silver nanostructured plates (FSNP) and also on Au(111) thin films. The cells were fixed by means of an ethanol dehydration train, then characterized by atomic force microscopy (AFM) and surface-enhanced Raman scattering (SERS). SERS results showed the same tendency as CLSM findings (SKBR3 > MCF-7 > T47D > MDA-MB-231), especially when the Raman peak associated with phenylalanine amino acid (1002 cm−1) was monitored. Given the high selectivity and high sensitivity of SERS with a functionalized silver nanostructured plate (FSNP), we propose this method for identifying the presence of HER2 and consequently, of breast cancer cells.

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Section: Resultssupporting

confidence: 90%

Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering

et al. 2018

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“…1. This study is designed to investigate the cell killing efficacy of PDT using our actively targeted nanoformulation (PLGA-PEG hTf BPD, referred to as "ANP") and compare it to both BPD in its free form (referred to as "free BPD") and passively targeted (PLGA-PEG BPD or "PNP") nanoparticles on TNBC cell line MDA-MB-231 (34)(35)(36)(37)(38)(39).…”

Section: Introductionmentioning

confidence: 99%

Remotely Phototriggered, Transferrin‐Targeted Polymeric Nanoparticles for the Treatment of Breast Cancer

Jadia

Kydd

Rai

2018

Photochem & Photobiology

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Triple-negative breast cancer (TNBC) has the worst prognosis among all subtypes of breast cancer. Currently, no targeted treatment has been approved for TNBC. The goal of this study was to design a remotely triggered, targeted therapy for TNBC using polymeric nanoparticles and light. Active targeting of TNBC was achieved by conjugating the nanoparticles to a peptide (hTf) that binds to the transferrin receptor, which is overexpressed in TNBC. Photodynamic therapy (PDT) was explored for TNBC treatment by remotely triggering benzoporphyrin derivative monoacid (BPD), a photosensitizer, using near-infrared light. In this study, we investigated the use of actively targeting polymeric nanoparticles for PDT against TNBC using in vitro imaging and cytotoxicity studies. Fluorescence imaging confirmed that the BPD-loaded nanoparticles showed greater fluorescence in TNBC cells compared to free BPD, but more importantly, actively targeted nanoparticles displayed stronger fluorescence compared to passively targeted nanoparticles. Moreover, fluorescence imaging following competition with empty targeted nanoparticles validated the specificity of the targeted nanoparticles for TNBC cells. The PDT killing results were in line with the fluorescence imaging results, where actively targeting nanoparticles exhibited the highest phototriggered cytotoxicity in TNBC cells, making them an attractive nanoplatform for TNBC treatment.

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“…Together, these findings indicate that BIRC7 promoted the progression and metastasis of breast cancer through the regulation of EMT by activating the p38/GSK3β pathway. A deeper understanding of the role of BIRC7-induced EMT in breast cancer may provide effective targets for therapy, especially in triple-negative breast cancer (Han et al, 2017, Etti et al, 2017).…”

Section: Breast Cancermentioning

confidence: 99%

BIRC7 (baculoviral IAP repeat containing 7)

Rigato¹,

Branco²,

Silva³

et al. 2020

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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BIRC7, also known as livin, is a member of the Inhibitor of Apoptosis Protein (IAP) family and is linked to the prevention of cell death induced by apoptosis, by directly or indirectly preventing caspase activity. In general, as most IAPs, BIRC7 expression is not detectable in normal differentiated adult tissues, with the exception of placenta, spleen, lymph nodes and developing embryonic tissues. On the other hand, BIRC7 overexpression has been reported in a variety of tumor types, in which it is associated to malignancy and chemoresistance. Currently, there are some unanswered questions about BIRC7, including its interaction with caspases, a potential paradoxical role in the apoptotic process, and specific functions/affinities of the BIRC7α and BIRC7β splice variants. Moreover, several studies have demonstrated the value of BIRC7 as a therapeutic target in a number of cancer types. This review mainly focuses on the role of BIRC7 in cancer cell biology and its clinical significance, demonstrating aspects of its DNA/RNA and protein, as well as its relevance in cancer diagnosis and prognosis.

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The molecular mechanism of the anticancer effect of Artonin E in MDA-MB 231 triple negative breast cancer cells

Cited by 42 publications

References 59 publications

Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering

Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering

Remotely Phototriggered, Transferrin‐Targeted Polymeric Nanoparticles for the Treatment of Breast Cancer

BIRC7 (baculoviral IAP repeat containing 7)

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