The molecular mechanism of synaptic activity‐induced astrocytic volume transient

Woo, Junsung; Jang, Minwoo Wendy; Lee, Jaekwang; Koh, Won‐Jung; Mikoshiba, Katsuhiko; Lee, C. Justin

doi:10.1113/jp279741

Cited by 10 publications

(13 citation statements)

References 64 publications

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“…We, therefore, compare and discuss the properties that make particular GECIs useful for particular applications, which should help in selecting from yet newer GECIs and not only from those in Figures 1, 2. In the figurative summaries, we have highlighted those references in which GECIs have been published in astrocytes (Atkin et al, 2009;Haustein et al, 2014;Kanemaru et al, 2014;Monai et al, 2016;Nakayama et al, 2016;Srinivasan et al, 2016;Stobart et al, 2018;Woo et al, 2020).…”

Section: A Field Guide To Gecis For Use In Glial Cellsmentioning

confidence: 99%

Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Lohr

Beiersdorfer

Fischer

et al. 2021

Front. Cell. Neurosci.

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Ca2+ imaging is the most frequently used technique to study glial cell physiology. While chemical Ca2+ indicators served to visualize and measure changes in glial cell cytosolic Ca2+ concentration for several decades, genetically encoded Ca2+ indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca2+ indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.

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Section: A Field Guide To Gecis For Use In Glial Cellsmentioning

confidence: 99%

Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Lohr

Beiersdorfer

Fischer

et al. 2021

Front. Cell. Neurosci.

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“…Recently, we postulated that diffusion MRI can detect the astrocyte volume change because astrocytes undergo dramatic volume changes as a result of fluid flux associated with extracellular K + release and subsequent activation of the astrocyte Na–K–Cl cotransporter (NKCC1), Kir4.1 inwardly rectifying potassium channel, and/or the Na + /K + ATPase [ 168 , 169 , 170 , 171 , 172 , 173 ]. In addition, AQP4 activity may contribute to these volume changes by mediating ISF–CSF exchange [ 174 ].…”

Section: Potential Noninvasive and Mesoscopic Astrocyte Imaging Using Mri And Positron Emission Tomography (Pet)mentioning

confidence: 99%

Potential of Multiscale Astrocyte Imaging for Revealing Mechanisms Underlying Neurodevelopmental Disorders

Kumamoto

Tsurugizawa

2021

IJMS

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Astrocytes provide trophic and metabolic support to neurons and modulate circuit formation during development. In addition, astrocytes help maintain neuronal homeostasis through neurovascular coupling, blood–brain barrier maintenance, clearance of metabolites and nonfunctional proteins via the glymphatic system, extracellular potassium buffering, and regulation of synaptic activity. Thus, astrocyte dysfunction may contribute to a myriad of neurological disorders. Indeed, astrocyte dysfunction during development has been implicated in Rett disease, Alexander’s disease, epilepsy, and autism, among other disorders. Numerous disease model mice have been established to investigate these diseases, but important preclinical findings on etiology and pathophysiology have not translated into clinical interventions. A multidisciplinary approach is required to elucidate the mechanism of these diseases because astrocyte dysfunction can result in altered neuronal connectivity, morphology, and activity. Recent progress in neuroimaging techniques has enabled noninvasive investigations of brain structure and function at multiple spatiotemporal scales, and these technologies are expected to facilitate the translation of preclinical findings to clinical studies and ultimately to clinical trials. Here, we review recent progress on astrocyte contributions to neurodevelopmental and neuropsychiatric disorders revealed using novel imaging techniques, from microscopy scale to mesoscopic scale.

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“… 1 Suzuki and Mizuno, 2004 ; 2 Suzuki, 2006 ; 3 Sabirov and Okada, 2009 ; 4 Han et al, 2019 ; 5 Woo et al, 2020 . …”

Section: Structure and Biochemical Function Of Tweety Family Proteinsmentioning

confidence: 99%

“…All three murine Tweety paralogs display activity of swelling-dependent volume-regulated anion channels (VRAC swell ) in mouse astrocyte primary culture when assessed by whole-cell patch-clamp recording and shRNA-mediated knockdown diminished current (Han et al, 2019 ; Supplementary Table 1 ). Additionally, expression of each TTYH in HEK293T and CHO-K1 cells resulted in an I Cl, Swell similar to that of native astrocytes, and all three Tweety paralogs were shown to be involved in the regulated volume decrease through a VRAC current in hippocampal astrocytes (Han et al, 2019 ; Woo et al, 2020 ). Bae et al ( 2019 ) found that TTYH1 and TTYH2 are both responsible for generating VRAC currents in the SNU-601 gastric cancer cell line after using CRISPR-Cas9 technology to delete exon 7 of the TTYH1 gene and exons 2 and 3 of the TTYH2 gene, which reduced VRAC currents in the resulting knockout cells.…”

Section: Structure and Biochemical Function Of Tweety Family Proteinsmentioning

confidence: 99%

The tweety Gene Family: From Embryo to Disease

Nalamalapu

Yue

Stone

et al. 2021

Front. Mol. Neurosci.

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The tweety genes encode gated chloride channels that are found in animals, plants, and even simple eukaryotes, signifying their deep evolutionary origin. In vertebrates, the tweety gene family is highly conserved and consists of three members—ttyh1, ttyh2, and ttyh3—that are important for the regulation of cell volume. While research has elucidated potential physiological functions of ttyh1 in neural stem cell maintenance, proliferation, and filopodia formation during neural development, the roles of ttyh2 and ttyh3 are less characterized, though their expression patterns during embryonic and fetal development suggest potential roles in the development of a wide range of tissues including a role in the immune system in response to pathogen-associated molecules. Additionally, members of the tweety gene family have been implicated in various pathologies including cancers, particularly pediatric brain tumors, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. Here, we review the current state of research using information from published articles and open-source databases on the tweety gene family with regard to its structure, evolution, expression during development and adulthood, biochemical and cellular functions, and role in human disease. We also identify promising areas for further research to advance our understanding of this important, yet still understudied, family of genes.

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The molecular mechanism of synaptic activity‐induced astrocytic volume transient

Cited by 10 publications

References 64 publications

Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Potential of Multiscale Astrocyte Imaging for Revealing Mechanisms Underlying Neurodevelopmental Disorders

The tweety Gene Family: From Embryo to Disease

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