The molecular mechanism of human stem cell-derived extracellular vesicles in retinal repair and regeneration

Yang, Mei; Peng, Guang-Hua

doi:10.1186/s13287-023-03319-2

Cited by 3 publications

(5 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, these two methods have limitations in clinical application. Patients are prone to nausea, vomiting, temporary hair loss, and other symptoms [ 117 ]. Furthermore, the protein transduction domain (PTD) transduction domain can also mediate SOD transduction [ 118 ].…”

Section: Problems and Research Progress Of Sods Applicationmentioning

confidence: 99%

The Applications and Mechanisms of Superoxide Dismutase in Medicine, Food, and Cosmetics

Zheng,

Liu,

Zhang

et al. 2023

Antioxidants

View full text Add to dashboard Cite

Superoxide dismutase (SOD) is a class of enzymes that restrict the biological oxidant cluster enzyme system in the body, which can effectively respond to cellular oxidative stress, lipid metabolism, inflammation, and oxidation. Published studies have shown that SOD enzymes (SODs) could maintain a dynamic balance between the production and scavenging of biological oxidants in the body and prevent the toxic effects of free radicals, and have been shown to be effective in anti-tumor, anti-radiation, and anti-aging studies. This research summarizes the types, biological functions, and regulatory mechanisms of SODs, as well as their applications in medicine, food production, and cosmetic production. SODs have proven to be a useful tool in fighting disease, and mimetics and conjugates that report SODs have been developed successively to improve the effectiveness of SODs. There are still obstacles to solving the membrane permeability of SODs and the persistence of enzyme action, which is still a hot spot and difficulty in mining the effect of SODs and promoting their application in the future.

show abstract

Section: Problems and Research Progress Of Sods Applicationmentioning

confidence: 99%

The Applications and Mechanisms of Superoxide Dismutase in Medicine, Food, and Cosmetics

Zheng,

Liu,

Zhang

et al. 2023

Antioxidants

View full text Add to dashboard Cite

show abstract

“…As a cell-free therapy, EVs can avoid the risk of immune rejection and tumor formation [ 8 ]. EVs are simpler than their stem cell sources, and they have low immunogenicity due to their lower content in membrane-bound proteins [ 47 ].…”

Section: Advantages Of Evs Applicationsmentioning

confidence: 99%

“…With the further development of stem cell research, nevertheless, researchers have found that SCs may lead to the formation of tumors and disease transmission when applied, and that immune rejection, stringent preservation and transport conditions further limit the application of SCs [ 5 – 7 ]. For these reasons, stem cell-derived extracellular vesicles (EVs) with low toxicity and low immunogenicity [ 8 ] have attracted the attention of researchers.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous experimental studies showed that EVs derived from SCs (SC-EVs), especially mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells [ 18 ], played a significant role in the treatment of respiratory diseases [ 19 , 20 ], kidney diseases [ 21 – 23 ], liver diseases [ 24 , 25 ], neurological diseases [ 26 ], cardiovascular diseases [ 27 ], and other diseases. As a cell-free component, EVs have high structural and compositional stability, and the ability to target injured cells, overcoming the shortcomings of stem cell therapy [ 8 ]. It is a potential alternative to stem cell therapy and a promising next-generation treatment.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Advances in the application of extracellular vesicles derived from three-dimensional culture of stem cells

Chen,

Wu,

Jin

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and potential tumorigenicity. Extracellular vesicles (EVs), a key paracrine component of stem cell potency, overcome the drawbacks of stem cell applications as a cell-free therapeutic agent and play an important role in treating various diseases. However, EVs derived from two-dimensional (2D) planar culture of SCs have low yield and face challenges in large-scale production, which hinders the clinical translation of EVs. Three-dimensional (3D) culture, given its ability to more realistically simulate the in vivo environment, can not only expand SCs in large quantities, but also improve the yield and activity of EVs, changing the content of EVs and improving their therapeutic effects. In this review, we briefly describe the advantages of EVs and EV-related clinical applications, provide an overview of 3D cell culture, and finally focus on specific applications and future perspectives of EVs derived from 3D culture of different SCs. Graphical Abstract

show abstract

“…These vesicles are secreted by various cell types, including immune, stem, and cancer cells, and they carry a diverse array of contents such as proteins, nucleic acids (DNA, mRNA, and microRNAs), and other biologically active compounds. EXOs are integral to the complex interactions among different tissues, significantly impacting tissue repair and regeneration [ 20 – 22 ]. Additionally, in the context of tissue injury or inflammation, cells release EXOs loaded with anti-inflammatory agents, thereby helping to modulate the inflammatory response and support tissue homeostasis [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Bio-nanoparticles loaded with synovial-derived exosomes ameliorate osteoarthritis progression by modifying the oxidative microenvironment

Cao,

Li,

Zhang

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

Background and aims Osteoarthritis (OA) is a prevalent degenerative joint disorder, marked by the progressive degeneration of joint cartilage, synovial inflammation, and subchondral bone hyperplasia. The synovial tissue plays a pivotal role in cartilage regulation. Exosomes (EXOs), small membrane-bound vesicles released by cells into the extracellular space, are crucial in mediating intercellular communication and facilitating the exchange of information between tissues. Our study aimed to devise a hydrogel microsphere infused with SOD3-enriched exosomes (S-EXOs) to protect cartilage and introduce a novel, effective approach for OA treatment. Materials and methods We analyzed single-cell sequencing data from 4247 cells obtained from the GEO database. Techniques such as PCR, Western Blot, immunofluorescence (IF), and assays to measure oxidative stress levels were employed to validate the cartilage-protective properties of the identified key protein, SOD3. In vivo, OA mice received intra-articular injections of S-EXOs bearing hydrogel microspheres, and the effectiveness was assessed using safranine O (S.O) staining and IF. Results Single-cell sequencing data analysis suggested that the synovium influences cartilage via the exocrine release of SOD3. Our findings revealed that purified S-EXOs enhanced antioxidant capacity of chondrocytes, and maintained extracellular matrix metabolism stability. The S-EXO group showed a significant reduction in mitoROS and ROS levels by 164.2% (P < 0.0001) and 142.7% (P < 0.0001), respectively, compared to the IL-1β group. Furthermore, the S-EXO group exhibited increased COL II and ACAN levels, with increments of 2.1-fold (P < 0.0001) and 3.1-fold (P < 0.0001), respectively, over the IL-1β group. Additionally, the S-EXO group showed a decrease in MMP13 and ADAMTS5 protein expression by 42.3% (P < 0.0001) and 44.4% (P < 0.0001), respectively. It was found that S-EXO-containing hydrogel microspheres could effectively deliver SOD3 to cartilage and significantly mitigate OA progression. The OARSI score in the S-EXO microsphere group markedly decreased (P < 0.0001) compared to the OA group. Conclusion The study demonstrated that the S-EXOs secreted by synovial fibroblasts exert a protective effect on chondrocytes, and microspheres laden with S-EXOs offer a promising therapeutic alternative for OA treatment.

show abstract

The molecular mechanism of human stem cell-derived extracellular vesicles in retinal repair and regeneration

Cited by 3 publications

References 102 publications

The Applications and Mechanisms of Superoxide Dismutase in Medicine, Food, and Cosmetics

The Applications and Mechanisms of Superoxide Dismutase in Medicine, Food, and Cosmetics

Advances in the application of extracellular vesicles derived from three-dimensional culture of stem cells

Bio-nanoparticles loaded with synovial-derived exosomes ameliorate osteoarthritis progression by modifying the oxidative microenvironment

Contact Info

Product

Resources

About