The molecular mechanism of fat accumulation changes in Black-Bone chickens with different energy intakes

Abstract

To investigate the molecular mechanism by which diets of different energy levels alter fat accumulation, 120 Black-Bone chickens were divided into 3 groups and fed diets with varying energy levels for six weeks (low: 3.02 Mcal/kg, moderate: 3.22 Mcal/kg, high: 3.42 Mcal/kg). The high-energy diet increased the concentration of blood lipids. Furthermore, high-energy intake inhibited the activities of several serum lipases. Histological sections showed over-synthesis of lipids in the livers of chickens fed high-energy diets. Data from western blotting and PCR analyses indicated that key factors for lipogenesis, regulatory transcription factors and fatty acid transporters, were up-regulated, while key factors for lipolysis were down-regulated. Chickens fed low-energy diets showed opposite results. In conclusion, varying energy levels of diets affect fat accumulation in Black-Bone chickens through changed expression of metabolic regulators.

Improvement in the carcass traits and meat quality of growing-finishing Rongchang pigs by conjugated linoleic acid through altered gene expression of muscle fiber types

“…The mRNA expression of some key factors in muscle fiber development (MyHC1, MyHC2a, MyHC2b, and MyHC2x) were measured using real-time polymerase chain reaction (PCR), as described previously (Qi et al, 2012). Real-time PCR was performed using an ABI 7300 system (ABI, USA) with a 20-μL reaction mixture containing 10.0 μL SYBR Premix Ex Taq TM II (TaKaRa, Japan), 0.8 μL forward primer, 0.8 μL reverse primer, 2 μL template cDNA, 0.4 μL dye, and 6 μL ddH 2 O.…”