The molecular epidemiology of four outbreaks of porcine pasteurellosis

Blackall, P. J.; Fegan, Narelle; Pahoff, J.L.; Storie, G J; McIntosh, G.B.; Cameron, R. D. A.; O’Boyle, D.; Frost, A. J.; Bara, Mamta; Marr, G.; Holder, J.

doi:10.1016/s0378-1135(99)00192-3

Cited by 40 publications

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“…The remaining 18 % of the strains were associated with just six OMP-types. The relatively low degree of diversity of the OMP profiles was not unexpected, because porcine P. multocida strains have previously been shown to have limited genetic diversity (Gardner et al, 1994;Fussing et al, 1999;Rubies et al, 2002;Zhao et al, 1992;Harel et al, 1990;Bowles et al, 2000;Blackall et al, 2000). The association of a small number of OMP-types with the majority of the strains suggests that a relatively small number of virulent groups (or clones) are responsible for most cases of infection (discussed in further detail below).…”

Section: Discussionmentioning

confidence: 91%

“…Genetic diversity of porcine strains of P. multocida has been examined in previous studies by restriction endonuclease analysis and ribotyping (Gardner et al, 1994;Fussing et al, 1999;Rubies et al, 2002;Zhao et al, 1992;Djordjevic et al, 1998;Harel et al, 1990;Donnio et al, 1999;Blackall et al, 2000). A comparative analysis of OMP variation in a large population of P. multocida strains associated with pneumonia and PAR has not been undertaken.…”

Section: Discussionmentioning

confidence: 99%

“…A number of different methods such as bacteriophage typing, plasmid profiling, restriction endonuclease analysis, ribotyping and analysis of outer-membrane proteins (OMPs) have been used to examine diversity among P. multocida strains associated with either PAR (Gardner et al, 1994;Fussing et al, 1999;Harel et al, 1990;Donnio et al, 1999;Nielsen & Rosdahl, 1990;Lugtenberg et al, 1984;Vasfi Marandi & Mittal, 1995;Bowles et al, 2000) or pneumonia (Rubies et al, 2002;Zhao et al, 1992;Blackall et al, 2000). However, there have been very few comparative studies of P. multocida isolates derived from both PAR and pneumonia (Djordjevic et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to isolates associated with PAR, P. multocida strains isolated from pneumonic lungs are usually non-toxigenic and of capsular type A (Pijoan et al, 1983(Pijoan et al, , 1984Rubies et al, 2002;Zhao et al, 1992 Despite the obvious differences between pneumonia and PAR and their association with P. multocida strains of different capsular types and toxin status, very little is known about the relationships of isolates responsible for each of these diseases (Djordjevic et al, 1998). A number of different methods such as bacteriophage typing, plasmid profiling, restriction endonuclease analysis, ribotyping and analysis of outer-membrane proteins (OMPs) have been used to examine diversity among P. multocida strains associated with either PAR (Gardner et al, 1994;Fussing et al, 1999;Harel et al, 1990;Donnio et al, 1999;Nielsen & Rosdahl, 1990;Lugtenberg et al, 1984;Vasfi Marandi & Mittal, 1995;Bowles et al, 2000) or pneumonia (Rubies et al, 2002;Zhao et al, 1992;Blackall et al, 2000). However, there have been very few comparative studies of P. multocida isolates derived from both PAR and pneumonia (Djordjevic et al, 1998).…”

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confidence: 99%

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Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

102

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One hundred and fifty-eight porcine strains of Pasteurella multocida, recovered primarily from cases of pneumonic pasteurellosis or progressive atrophic rhinitis (PAR) in England and Wales, were characterized by determination of their capsular types, presence or absence of the toxA gene and molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. Eighteen groups (clones) of strains were identified on the basis of specific combinations of capsular type, toxA status and outer-membrane protein (OMP)-type. The data provided evidence that different subpopulations of P. multocida are responsible for pneumonia and PAR in pigs. The majority (88 %) of cases of pneumonia were associated exclusively with non-toxigenic capsular type A strains of OMP-types 1.1, 2.1, 3.1 and 5.1 and capsular type D isolates of OMP-type 6.1. These strains were recovered from widespread geographical locations within England and Wales over a 12-year period and represented mostly single sporadic cases. The association of a small number of P. multocida variants with the majority of cases of porcine pneumonia suggests that these strains are not opportunistic pathogens of low virulence but represent primary pathogens with a relatively high degree of virulence. In contrast, the majority (76 %) of cases of PAR were associated with toxAcontaining capsular type D strains of OMP-type 4.1 and capsular type A and D strains of OMP-type 6.1. Toxigenic capsular type A strains associated with PAR and non-toxigenic capsular type A strains associated with pneumonia represent distinct subpopulations of P. multocida that can be differentiated by their OMP-types. The association of capsular types A and D with strains of the same OMP-types, and the absence and presence of the toxA gene in strains of the same OMP-types, suggest that horizontal transfer of capsular biosynthesis and toxA genes has occurred between strains representing certain subpopulations of P. multocida. INTRODUCTIONPasteurella multocida is the aetiological agent of progressive atrophic rhinitis (PAR) of swine and is of considerable economic importance to the pig-rearing industry throughout the world (Chanter & Rutter, 1989). PAR is characterized by atrophy of the nasal turbinate bones which, in severe cases, can lead to facial distortion. P. multocida strains associated with PAR usually produce a 145-kDa dermonecrotic toxin, which is encoded by the toxA gene (Lax et al., 1990;Buys et al., 1990). This toxin induces osteolysis in the turbinate bones and plays an important role in the pathogenesis of PAR (Kamp & Kimman, 1988). Toxigenic strains associated with PAR are most frequently of capsular type D (Eamens et al., 1988;Foged et al., 1988;Gardner et al., 1994;Lariviere et al., 1992), although toxigenic isolates of capsular type A can also be involved in the disease (Fussing et al., 1999;Sakano et al., 1992). Non-toxigenic P. multocida strains are not usually associated with PAR and confirmation of toxin production is important for the diagnosis and control of the disea...

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

102

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“…multocida is a gram-negative rod with bipolar staining that affects cattle (Frank, 1989), pigs (Blackall et al, 2000), rabbits (Langan et al, 2000), and humans (Weber et al, 1984), in addition to fowl (Christensen et al, 2008). P. multocida includes capsulated and noncapsulated types, with the former being more virulent than the latter (Boyce and Adler, 2000;Chung et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

An outbreak of chronic fowl cholera in broiler breeder chickens in Korea

Kim¹,

Yoon²,

Cho³

et al. 2011

Korean Journal of Veterinary Service

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Fowl cholera is a contagious acute and chronic disease caused by Pasteurella multocida in both domesticated and wild birds. Acute fowl cholera in both chickens and wild birds has recently been documented in Korea, but the chronic form has not been reported in Korea until now. This study describes the first outbreak of chronic fowl cholera in 13-week-old Arbor Acre broiler breeder chickens submitted to the College of Veterinary Medicine, Kyungpook National University in April 2006. The clinical signs of the affected flock of 9,621 chickens were lameness caused by swollen hock joints, diarrhea, ruffled feathers, and an average weekly mortality of 1.0%. At necropsy, purulent or caseous exudates were found in the hock and wing joints, humerus, and eyes, and severe pneumonia and pericarditis were discovered. Eleven bacterial isolates obtained from the liver, joint, infraorbital sinus and sternal bursa of the submitted chickens were all identified as Pasteurella multocida based on their physiological and biochemical characteristics. Five isolates were examined for antimicrobial susceptibility against 21 different antimicrobial agents including ampicillin. All were resistant to kanamycin, neomycin, and streptomycin, and some were resistant to gentamicin. The tested isolates were all susceptible to the other 17 antimicrobial agents. All 11 isolates were capsular serogroup A based on multiplex polymerase chain reaction. In addition, two of five isolates used in the antimicrobial susceptibility test were identified as somatic serotype 1 by an agar gel diffusion precipitin test, while the others were non-typable.

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