The molecular epidemiology of bovine rotaviruses circulating in Iran: a two-year study

Abstract

Bovine group A rotavirus (bovine RVA) is recognized as a major cause of severe gastroenteritis in newborn calves. The purpose of this study was to estimate the prevalence and identify the genotypes of circulating bovine RVA in newborn diarrheic calves. Two hundred fifty-three stool samples of diarrheic calves up to 1 month old were collected from 42 industrial dairy farms in two Iranian provinces during March 2010 to February 2012. All collected samples were screened for the presence of bovine RVA by RT-PCR, and the G and P genotypes were determined by semi-nested multiplex RT-PCR assay. The results of RT-PCR indicated that 49.4 % (125 out of 253) of the samples were positive for bovine RVA. The G and P genotyping of a subset of positive samples (n = 85) by semi-nested multiplex RT-PCR revealed that G6 (55.3 %) and G10 (43.5 %) and P[5] (51.8 %) and P[11] (27 %) were the most prevalent G and P genotypes, respectively. G6P[5] was the dominant genotype (35.3 %), followed by G10P[5], G10P[11] and G6P[11], with prevalence rates of 16.5 %, 15.3 % and 10.6 %, respectively. Sequence analysis of 20 VP7 and four VP4 genes showed highest nucleotide sequence identity with the corresponding genes of strains RVA/Cow-tc/GBR/UK/1973/G6P7[5] and RVA/Cow-tc/USA/B223/XXXX/G10P[11]. The results of this study reveal the diversity of G and P genotypes in bovine RVA samples from diarrheic Iranian calves and expands our knowledge of bovine RVA infections in the Middle East. These results also highlight the importance of producing of an effective rotavirus vaccine and its inclusion in the national cattle immunization program.

Molecular characterization of unusual G10P[33], G6P[14] genomic constellations and evidence of zooanthroponosis in bovines

“…The predominance of G10P[11] combination observed in this study is in concurrence with the previous reports from India (Beg et al, 2010; Minakshi et al, 2015, Tatte et al, 2019). However, these findings are in contrast with Indian studies with G3P[11] predominance (Malik et al, 2012; Sravani et al, 2014) and reports from Iran (Pourasgari et al ., 2016) and Ireland with G6P[5] as major genotype (Collins et al ., 2014). Interestingly, the VP6 gene of G10P[11] study strains grouped in different cluster than prototype Bovine strains, in I2 genotype.…”

High prevalence and genomic characteristics of G6P[1] Bovine Rotavirus A in yak in China

“…The results showed that BRVA has been circulating widely among yak. Previous studies have shown that BRVA strains possessing G6, G8, and G10 genotypes in combination with P[1], P[5] and P[11] have been commonly detected in cattle [5, 25]. Similar data were reported in dairy calves in China, in which the most common combinations were G6P[5], G10P[5] and G6P[11] [26].…”

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

“…Rotavirus A was detected in feces and intestinal contents collected from dairy and beef calves with a frequency of 57%, which was higher than reports from Argentina and Brazil (17–42%) [ 19 , 20 , 21 , 22 ], and other geographic regions (20–49%) [ 7 , 23 , 24 , 25 ]. On the other hand, in Australia, the frequency of RVA detection was 80%, which is higher than the detected in our study [ 6 ].…”