The molecular effects of ultrasound on the expression of cellular proteome

Fang, Guiting; Chen, Weijian; Zhong, Xing; Long, Yan; Qin, Huaming; Ye, Jinshao

doi:10.1016/j.scitotenv.2020.137439

Cited by 6 publications

(2 citation statements)

References 46 publications

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“…In fact, the ammonia oxidation ratio in both reactors was above 95%, and the ammonia oxidation rate remained stable in ultrasonic samples, with an average value [5.95 mg N/(h•g MLSS)] even slightly higher than that of the samples without sonication [4.44 mg N/(h•g MLSS)]. A similar promotional effect of low-intensity ultrasound on ammonium oxidation was also reported in other metabolic pathways, such as hydrogen production (Yin et al, 2018), iron-sulfur cluster biosynthesis (Zhang et al, 2020), and ATP release (Belcik et al, 2017).…”

Section: Performance Of Reactor During Partial Nitrificationsupporting

confidence: 68%

Changes in the Species and Functional Composition of Activated Sludge Communities Revealed Mechanisms of Partial Nitrification Established by Ultrasonication

Xue

Zheng

et al. 2022

Front. Microbiol.

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To achieve energy-efficient shortcut nitrogen removal of wastewater in the future, selective elimination of nitrite-oxidizing bacteria (NOB) while enriching ammonia-oxidizing microorganisms is a crucial step. However, the underlying mechanisms of partial nitrification are still not well understood, especially the newly discovered ultrasound-based partial nitrification. To elucidate this issue, in this study two bioreactors were set up, with one established partial nitrification by ultrasonication while the other didn't. During the operation of both reactors, the taxonomic and functional composition of the microbial community were investigated through metagenomics analysis. The result showed that during ultrasonic partial nitrification, ammonia-oxidizing archaea (AOA), Nitrososphaerales, was enriched more than ammonia-oxidizing bacteria (AOB), Nitrosomonas. The enrichment of microorganisms in the community increased the abundance of genes involved in microbial energy generation from lipid and carbohydrates. On the other hand, the abundance of NOB, Nitrospira and Nitrolancea, and Comammox Nitrospira decreased. Selective inhibition of NOB was highly correlated with genes involved in signal transduction enzymes, such as encoding histidine kinase and serine/threonine kinase. These findings provided deep insight into partial nitrification and contributed to the development of shortcut nitrification in wastewater treatment plants.

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Section: Performance Of Reactor During Partial Nitrificationsupporting

confidence: 68%

Changes in the Species and Functional Composition of Activated Sludge Communities Revealed Mechanisms of Partial Nitrification Established by Ultrasonication

Xue

Zheng

et al. 2022

Front. Microbiol.

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“…43 In addition, the process could also produce hydrogen peroxide and free radicals, which can enter cells through activated mechanosensitive channels, leading to potential disruption of the heat shock response system and the oxidative response system. 24 The integration of these mechanisms may stimulate a series of pathways in response, 46,47 which can help expand differences of strains. For instance, mechanical damage may release the internal substances of bacteria.…”

Section: ■ Introductionmentioning

confidence: 99%

Phenotyping Bacteria through a Black-Box Approach: Amplifying Surface-Enhanced Raman Spectroscopy Spectral Differences among Bacteria by Inputting Appropriate Environmental Stress

Wei

Wang

et al. 2022

Anal. Chem.

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Surface-enhanced Raman spectroscopy (SERS) stands out in the field of microbial analysis due to its rich molecular information, fast analysis speed, and high sensitivity. However, achieving strain-level differentiation is still challenging because numerous bacterial species inevitably have very similar SERS profiles. Here, a method inspired by the black-box theory was proposed to boost the spectral differences, where the undifferentiated bacteria was considered as a type of black-box, external environmental stress was used as the input, and the SERS spectra of bacteria exposed to the same stress was output. For proof of the concept, three types of environmental stress were explored, i.e., ethanol, ultraviolet light (UV), and ultrasound. Enterococcus faecalis (E. faecalis) and three types of Escherichia coli (E. coli) were all subjected to the stimuli (stress) before SERS measurement. Then the collected spectra were processed only by simple principal component analysis (PCA) to achieve differentiation. The results showed that appropriate stress was beneficial to increase the differences in bacterial SERS spectra. When sonication at 490 W for 60 s was used as the input, the optimal differentiation of bacteria at the species (E. faecalis and E. coli) and strain-level (three E. coli) can be achieved.

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Beyond traditional pathways: Uncovering ultrasonication’s novel routes to enhance hydrogen yields in Clostridium pasteurianum

Song,

Ginige,

Yu Cheng

et al. 2024

Chemical Engineering Journal

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The molecular effects of ultrasound on the expression of cellular proteome

Cited by 6 publications

References 46 publications

Changes in the Species and Functional Composition of Activated Sludge Communities Revealed Mechanisms of Partial Nitrification Established by Ultrasonication

Changes in the Species and Functional Composition of Activated Sludge Communities Revealed Mechanisms of Partial Nitrification Established by Ultrasonication

Phenotyping Bacteria through a Black-Box Approach: Amplifying Surface-Enhanced Raman Spectroscopy Spectral Differences among Bacteria by Inputting Appropriate Environmental Stress

Beyond traditional pathways: Uncovering ultrasonication’s novel routes to enhance hydrogen yields in Clostridium pasteurianum

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