The molecular detection of circulating tumour cells

Johnson, P. W. M.; Burchill, Susan A.; Selby, Peter J.

doi:10.1038/bjc.1995.324

Cited by 144 publications

(114 citation statements)

References 87 publications

(9 reference statements)

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“…Several reports have demonstrated that PCR is a useful tool for the detection of circulating cancer cells in solid tumours (Johnson et al, 1995). In gastrointestinal tumours, in which cancer-specific mutations in DNA have not yet been found, it is necessary to use reverse transcriptase to prepare cDNA from peripheral blood mRNA for identifying tissue-specific gene expression.…”

Section: Discussionmentioning

confidence: 99%

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Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Castells

Boix²,

Bessa³

et al. 1998

Br J Cancer

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Section: Discussionmentioning

confidence: 99%

“…1991). Investigations devoted to studv this subpopulation of circulating, malignant cells have been limited until the advent of the reverse transcriptase polI merase chain reaction (RT-PCR) (Johnson et al 1995). Usinc this approach and targeting tissue-specific gene transcription.…”

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Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Castells

Boix²,

Bessa³

et al. 1998

Br J Cancer

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“…Various techniques, including morphology, flow cytometry and cytogenetics, have been used to detect disseminated tumour cells, but most of these have limited sensitivity and specificity (Frank et al, 1990;Diel et al, 1992;Johnson et al, 1995) and are time-consuming (Gross et al, 1995). Immunocytochemistry has also been used in an attempt to detect circulating breast carcinoma cells perioperatively, but sensitivity remains a concern with this technique (McCulloch et al, 1995;Choy and McCulloch, 1996).…”

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Putative markers for the detection of breast carcinoma cells in blood

El-Tahir

Mallinson²,

Birnie³

et al. 1998

Br J Cancer

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Summary The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy female volunteers. This RNA was screened for mRNA of MUC1, cytokeratin 19 (CK19) and CD44 (exons 8-11) by RT-PCR and the results validated by Southern blots. Variable degrees of expression of MUC1 and CD44 (exons 5-11) were detected in normal peripheral blood, rendering these genes non-specific for epithelial cells and therefore unsuitable for use as markers to detect breast carcinoma cells. Although CK1 9 mRNA was apparently specific, it was deemed unsuitable for use as a marker of breast cancer cells in light of its limited sensitivity. Furthermore, an attempt at using nested primers to increase sensitivity resulted in CK19 mRNA being detected after two amplification rounds in blood from healthy volunteers.

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“…Increasingly, reverse transcriptase polymerase chain reaction (RT -PCR) ( Figure 1A) for tumour-or tissue-specific targets is used to detect tumour cells in bone marrow, peripheral blood and stem cell harvests with greater sensitivity than more conventional methods (Johnson et al, 1995). There is now good evidence that using this method it is possible to detect disease of clinical significance (Burchill and Selby, 2000;Burchill et al, 2001).…”

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confidence: 99%

Comparison of the RNA-amplification based methods RT–PCR and NASBA for the detection of circulating tumour cells

Burchill

Perebolte²,

Johnston

et al. 2002

Br J Cancer

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Increasingly, reverse transcriptase polymerase chain reaction (RT–PCR) is used to detect clinically significant tumour cells in blood or bone marrow. This may result in a redefinition of disease-free and clinical relapse. However, its clinical utility may be limited by lack of automation or reproducibility. Recent studies have suggested nucleic acid sequence-based amplification of target RNA may be more robust. In this study, nucleic acid sequence-based amplification was established to detect melanoma, colorectal and prostate cancer cells. Nucleic acid sequence-based amplification and RT–PCR both successfully amplified target RNA in peripheral blood samples from patients with melanoma and colorectal cancer, but only RT–PCR detected PSA in blood samples from patients with prostate cancer. There was relatively good agreement between sample replicates analyzed by RT–PCR (Kappa values of one for tyrosinase, 0.67 for CK-20 and one for PSA), but less agreement when analyzed by nucleic acid sequence-based amplification. This may limit the routine use of NASBA for the detection of clinically significant disease. In summary, RT–PCR appears at present to be the most reliable and reproducible method for the detection of low-level disease in cancer patients, although prospective studies are warranted to assess the clinical utility of different molecular diagnostic methods. British Journal of Cancer (2002) 86 , 102–109. DOI: 10.1038/sj/bjc/6600014 www.bjcancer.com © 2002 The Cancer Research Campaign

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The molecular detection of circulating tumour cells

Cited by 144 publications

References 87 publications

Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Putative markers for the detection of breast carcinoma cells in blood

Comparison of the RNA-amplification based methods RT–PCR and NASBA for the detection of circulating tumour cells

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