The molecular controls of resolution of inflammation: what can we learn from zebrafish?

Renshaw, Stephen A.; Trushell, Daniel M. I; Ingham, Philip W.; Whyte, Moira K. B.

doi:10.1183/09059180.00010108

Cited by 2 publications

(2 citation statements)

References 4 publications

(3 reference statements)

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“…4), which is similar to the effect of MP in carrageenan-induced paw edema in rodents [57]. Together, our results showing the anti-edematous effects of MP, anti-TNF-α antibody, and AG support the hypothesis that zebrafish and rodents share basic inflammatory responses, which could be modulated by bioactive compounds, and that therefore the zebrafish model is suitable for use in the investigation of biomedical issues, especially in immunology [13], [14], [17], [22], [24]. Moreover, this study also confirms the ability of this model to identify anti-inflammatory compounds, and it would further be expected that novel compounds might be identified via this zebrafish model of inflammation.…”

Section: Discussionsupporting

confidence: 82%

A Novel Zebrafish Model to Provide Mechanistic Insights into the Inflammatory Events in Carrageenan-Induced Abdominal Edema

et al. 2014

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A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-α antibody (1∶5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds.

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Section: Discussionsupporting

confidence: 82%

A Novel Zebrafish Model to Provide Mechanistic Insights into the Inflammatory Events in Carrageenan-Induced Abdominal Edema

et al. 2014

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“…Neutrophil recruitment into the site of injury can be seen. This figure has been published previously in [9]. 552 S. A. Renshaw et al…”

Section: Figurementioning

confidence: 85%

Modeling Inflammation in the Zebrafish: How a Fish Can Help Us Understand Lung Disease

Renshaw

Loynes

Elworthy

et al. 2007

Experimental Lung Research

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Neutrophilic inflammation is responsible for much of the tissue damage seen in many lung diseases. For resolution of inflammation to occur, neutrophils must die by apoptosis, allowing their recognition and removal by macrophages. The molecular events controlling this important regulatory step are poorly understood, in large part due to the genetic intractability of the human neutrophil granulocyte. The authors have established a model of inflammation in the Zebrafish, which shares many features of the innate immune system with those of humans. Injury to the Zebrafish tailfin induces a reproducible and quantifiable inflammatory response, which resolves with kinetics similar to mammalian models of neutrophilic inflammation, including pulmonary inflammation. Pharmacological modulation of neutrophil apoptosis can modulate the outcome of experimentally induced inflammation. In addition, the authors have generated a construct that expresses green fluorescent protein under the myeloperoxidase promoter, allowing in vivo visualization of neutrophils during experimentally induced inflammation. The authors are also performing an unbiased forward genetic screen for mutants with defective resolution of inflammation, and to date have identified a number of putative mutants. Further study and characterization of these mutants is underway. The authors have thus established an important experimental link between apoptosis and resolution of inflammation in an in vivo system, and defined an important new model for the study of inflammation resolution. The authors hope that these tools will permit detailed study of the genetic controls of the resolution of inflammation, and provide insights with potential clinical utility.

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The molecular controls of resolution of inflammation: what can we learn from zebrafish?

Cited by 2 publications

References 4 publications

A Novel Zebrafish Model to Provide Mechanistic Insights into the Inflammatory Events in Carrageenan-Induced Abdominal Edema

A Novel Zebrafish Model to Provide Mechanistic Insights into the Inflammatory Events in Carrageenan-Induced Abdominal Edema

Modeling Inflammation in the Zebrafish: How a Fish Can Help Us Understand Lung Disease

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