The Molecular Control of Meiotic Chromosomal Behavior: Events in Early Meiotic Prophase in <i>Drosophila</i> Oocytes

Lake, Cathleen M.; Hawley, R. Scott

doi:10.1146/annurev-physiol-020911-153342

Cited by 87 publications

(106 citation statements)

References 190 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…In the 1920 th , the analysis of Drosophila meiosis started with the isolation of the first meiotic mutant c(3)G (Lake and Hawley 2012). C(3)G was finally characterized as a major component of the Drosophila SC quite recently.…”

Section: Drosophila Melanogaster Femalementioning

confidence: 99%

“…Additionally, polymerization of C(3)G depends on another protein which was described in 2008 as sole potential CE protein, Corona (Cona). It does not contain any coiled coil region but localizes to the outer edge of the electron dense region of the CE (Page et al 2008;Lake and Hawley 2012).…”

Section: Drosophila Melanogaster Femalementioning

confidence: 99%

“…The diagram is adapted from Schramm 2011. Webber et al 2004Gause et al 2010;Lake and Hawley 2012). In summary, the following model of the Drosophila SC was developed ( Figure 5-4).…”

Section: Drosophila Melanogaster Femalementioning

confidence: 99%

See 2 more Smart Citations

Evolutionary history of the mammalian synaptonemal complex

et al. 2016

View full text Add to dashboard Cite

Section: Drosophila Melanogaster Femalementioning

confidence: 99%

Section: Drosophila Melanogaster Femalementioning

confidence: 99%

See 1 more Smart Citation

Evolutionary history of the mammalian synaptonemal complex

et al. 2016

View full text Add to dashboard Cite

“…In these organisms, SC formation is independent of recombination and CO designation; moreover, both recombination initiation and interference is or can be implemented after SC formation (40)(41)(42)(43). In these cases, the interference process seems limited to patterning of COs, and not SC nucleation, suggesting that an important functional rationale for interference may relate to CO/chiasma formation (27).…”

Section: Complex Spatial Patterns Can Arise In a Single Interference-mentioning

confidence: 99%

Interference-mediated synaptonemal complex formation with embedded crossover designation

Zhang

Espagne

Muyt

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Biological systems exhibit complex patterns at length scales ranging from the molecular to the organismic. Along chromosomes, events often occur stochastically at different positions in different nuclei but nonetheless tend to be relatively evenly spaced. Examples include replication origin firings, formation of chromatin loops along chromosome axes and, during meiosis, localization of crossover recombination sites ("crossover interference"). We present evidence in the fungus Sordaria macrospora that crossover interference is part of a broader pattern that includes synaptonemal complex (SC) nucleation. This pattern comprises relatively evenly spaced SC nucleation sites, among which a subset are crossover sites that show a classical interference distribution. This pattern ensures that SC forms regularly along the entire length of the chromosome as required for the maintenance of homolog pairing while concomitantly having crossover interactions locally embedded within the SC structure as required for both DNA recombination and structural events of chiasma formation. This pattern can be explained by a threshold-based designation and spreading interference process. This model can be generalized to give diverse types of related and/or partially overlapping patterns, in two or more dimensions, for any type of object.spatial patterning | synapsis initiation site | recombination/synapsis | crossover designation M eiosis is the specialized cellular cycle that yields haploid gametes for sexual reproduction. A central feature of the meiotic program is recombination (1, 2). DNA/DNA recombination interactions, initiated by programmed double-strand breaks (DSBs), mediate the recognition and juxtaposition (pairing) of homologous chromosomes. A minority subset of these interactions matures into reciprocal crossover recombination products (COs); the remaining majority matures primarily into interhomolog non-crossover products (NCOs). COs promote genetic diversity but also are required for the segregation of homologous chromosomes (homologs) via their role in creating chiasmata (3).A nearly universal feature of meiosis is that COs occur along a particular chromosome at different positions in different meiotic nuclei. Nonetheless, along any given chromosome, COs tend to be evenly spaced. This pattern results from the reduced possibility that a second cross-over will occur if a crossover has occurred nearby. The existence of such a pattern was identified more than a century ago as the genetic phenomenon of CO interference (4, 5). In this phenomenon, occurrence of a CO in one genetic interval is accompanied by a reduced probability that another CO will occur along the same chromosome in a nearby interval. This effect implies the existence of communication along chromosomes, with an event at one position triggering occurrence of an "interference signal" that spreads outward, inhibiting occurrence of subsequent events nearby.A second central feature of the meiotic program is the synaptonemal complex (SC). This prominent structure link...

show abstract

“…The C terminus of each C(3)G homodimer is positioned within the LE (13,14), although how the C terminus interacts with the LE and other chromosome axis proteins is unclear. Immuno-EM analysis of the SC protein Corona (CONA) suggests that it runs as two parallel tracks on the outer edges of the CE (15). CONA interacts with a second TF-like protein known as Corolla, as shown by yeast two-hybrid analysis (16).…”

mentioning

confidence: 99%

Superresolution expansion microscopy reveals the three-dimensional organization of theDrosophilasynaptonemal complex

Cahoon

Wang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

127

117

View full text Add to dashboard Cite

The synaptonemal complex (SC), a structure highly conserved from yeast to mammals, assembles between homologous chromosomes and is essential for accurate chromosome segregation at the first meiotic division. In Drosophila melanogaster, many SC components and their general positions within the complex have been dissected through a combination of genetic analyses, superresolution microscopy, and electron microscopy. Although these studies provide a 2D understanding of SC structure in Drosophila, the inability to optically resolve the minute distances between proteins in the complex has precluded its 3D characterization. A recently described technology termed expansion microscopy (ExM) uniformly increases the size of a biological sample, thereby circumventing the limits of optical resolution. By adapting the ExM protocol to render it compatible with structured illumination microscopy, we can examine the 3D organization of several known Drosophila SC components. These data provide evidence that two layers of SC are assembled. We further speculate that each SC layer may connect two nonsister chromatids, and present a 3D model of the Drosophila SC based on these findings.synaptonemal complex | expansion microscopy | meiosis | sister chromatids | structured illumination microscopy

show abstract

The Molecular Control of Meiotic Chromosomal Behavior: Events in Early Meiotic Prophase in Drosophila Oocytes

Cited by 87 publications

References 190 publications

Evolutionary history of the mammalian synaptonemal complex

Evolutionary history of the mammalian synaptonemal complex

Interference-mediated synaptonemal complex formation with embedded crossover designation

Superresolution expansion microscopy reveals the three-dimensional organization of theDrosophilasynaptonemal complex

Contact Info

Product

Resources

About