The molecular characterization of antioxidant enzyme genes in Helicoverpa armigera adults and their involvement in response to ultraviolet-A stress

Wang, Yong; Wang, Lijun; Zhu, Zhihui; Ma, Weihua; Lei, Chaoliang

doi:10.1016/j.jinsphys.2012.06.012

Cited by 51 publications

(24 citation statements)

References 78 publications

(87 reference statements)

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“…Recently, Sang et al (2012) reported that UVA radiation could enhance expression of transcripts of some Hsp and P450 gene families. Furthermore, several antioxidant enzyme genes are signiÞcantly upregulated at the transcriptional level by UVA radiation, including copper zinc superoxide dismutase (Cu/ZnSOD), catalase (CAT), and glutathione peroxidase (GPX) (Wang et al 2012). Analyses at the mRNA level will help us better understand the gene expression proÞles of UVA-regulated genes.…”

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confidence: 99%

Identification and Transcriptional Profiling of Differentially Expressed Genes Associated With Response to Uva Radiation inDrosophila melanogaster(Diptera: Drosophilidae)

et al. 2013

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Ultraviolet A (UVA) radiation, the major component of solar ultraviolet (UV) radiation reaching the earth's surface, leads to negative effects in insects, such as oxidative stress, photoreceptor damage, and cell death. To better understand the molecular mechanisms of insect response to UVA radiation, suppression subtractive hybridization (SSH) and real-time quantitative polymerase chain reaction approaches were combined to reveal differential transcript expression in Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae). In this study, two subtractive cDNA libraries were constructed and sequenced, obtaining 131 high-quality unique expressed sequence tags (ESTs) that were up- or downregulated in D. melanogaster exposed to UVA radiation for 0.5 h. Of the 131 ESTs, 102 unique ESTs were differentially expressed and classified into 10 functional categories. The results showed that UVA radiation induces expression of genes related to stress and defense response and metabolism. Potential transcription factor binding motifs upstream of these genes are associated with multiple signaling pathways that may help the insect cope with the stress of UVA radiation. To our knowledge, this is the first analysis of insect response to UVA radiation at the transcriptional level. Our results reveal that UVA radiation influences the expression profiles of stress-responsive genes and provide further insights into the mechanisms of adaptive response to UVA radiation stress.

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confidence: 99%

Identification and Transcriptional Profiling of Differentially Expressed Genes Associated With Response to Uva Radiation inDrosophila melanogaster(Diptera: Drosophilidae)

et al. 2013

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“…32,33 Identification and upregulation of number of SOD activity isoforms in insects against various stresses have been reported previously. 14,18 Studies confirmed the presence of different SOD activity isoforms in several insect species. H. armigera MnsOD showed strong activity at highly alkaline ph.…”

Section: Discussionmentioning

confidence: 80%

“…Biochemical characterization of SODs along with the identification of individual enzyme isoform is an interesting aspect of research in insects. Although several studies from various insect species have reported the molecular characterization and responses of SODs against different types of stresses, [14][15][16][17][18] knowledge on the identification and characterization of SODs in H. armigera is lacking. Similarly, the role of SODs in insect resistance mechanism against insecticides is also not completely understood yet.…”

Section: Introductionmentioning

confidence: 99%

Superoxide dismutase activities in the midgut of Helicoverpa armigera larvae: identification and biochemical properties of a manganese superoxide dismutase

Hivrale¹,

Lomate²,

Sangole³

et al. 2015

OAIP

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Insects possess a complex network of enzymatic antioxidant systems protecting against reactive oxygen species generated during stress. Superoxide dismutases (SODs) are vital antioxidant enzymes converting superoxide into oxygen and hydrogen peroxide. Helicoverpa armigera is a polyphagous insect and has developed resistance to various phytochemicals and pesticides. Although SODs are studied in several insect species, their characterization has been reported in only a few insect species. Here, we attempted to identify and characterize SOD activity from H. armigera. Electrophoretic separation followed by nitroblue tetrazolium staining revealed the presence of five SOD activity isoforms in the midgut of H. armigera larvae. Total SOD activity was measured at the different larval developmental stages and found maximum at fourth instar and further declined at sixth instar. Total SOD activity was significantly increased by metal ions Mn, Fe, Zn, and Cu and reduced by Ca and Pb. The isoform with highest activity was identified as manganese SOD (MnSOD) because it was found insensitive to H 2 O 2 . The identified MnSOD was partially purified and characterized. After gel filtration chromatography, specific activity of the purified enzyme was found to be 6,348.0 U/mg/min with 26.61% yield. The molecular mass of purified enzyme was calculated to be ∼29.3 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH and temperature for the SOD activity were found to be around 11 and 60°C, respectively. The study could be useful to further understand the functional significance of SODs in the antioxidant mechanism of H. armigera.

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“…They interfere with the cholinergic nervous system and cause death because the effects of the neurotransmitter acetylcholine cannot be terminated by carbamylated acetyl cholinesterase. (Wang, et al, 2012). Sevin is also found to be a neurotoxin (Branch and Jacqz, 1986).…”

Section: Introductionmentioning

confidence: 98%

Histological and Ultra Structural Alterations of Gills of Freshwater Fish Channa punctatus (Bloch), On Exposure to Pesticide, Sevin

2016

IJSR

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Carbaryl (1-naphthyl methyl carbamate) is a carbamate pesticide. It is a white crystalline solid commonly called Sevin. The present study deals with the deleterious effects of 10.56ppm Sevin on the histopathological and ultra structural levels of the gills of Channa punctatus (1/10 th of LC 50 96 hrs).The histopathology of gill was studied under light and scanning electron microscopy. Sevin is a wide-spectrum carbamate pesticide which controls over 100 species of pests. The gills of Channa punctatus exposed to Sevin exhibited lamellar fusion at the tip of gill filaments. In addition, a few aneurisms were also observed in gill lamellae. The gills showed curved secondary gill filaments resulting in distension of the lamellae and the epithelial layer detached completely from the central portion of gill lamellae.

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The molecular characterization of antioxidant enzyme genes in Helicoverpa armigera adults and their involvement in response to ultraviolet-A stress

Cited by 51 publications

References 78 publications

Identification and Transcriptional Profiling of Differentially Expressed Genes Associated With Response to Uva Radiation inDrosophila melanogaster(Diptera: Drosophilidae)

Identification and Transcriptional Profiling of Differentially Expressed Genes Associated With Response to Uva Radiation inDrosophila melanogaster(Diptera: Drosophilidae)

Superoxide dismutase activities in the midgut of Helicoverpa armigera larvae: identification and biochemical properties of a manganese superoxide dismutase

Histological and Ultra Structural Alterations of Gills of Freshwater Fish Channa punctatus (Bloch), On Exposure to Pesticide, Sevin

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