The Molecular Biology of Brain Metastasis

Rahmathulla, Gazanfar; Toms, Steven; Weil, Robert J.

doi:10.1155/2012/723541

Cited by 52 publications

(61 citation statements)

References 106 publications

(126 reference statements)

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“…Most Map2 + cells, if not all, are IQGAP1 + stained ( Figure 3C,D,K-L and Supplementary Materials, Figure S1), in addition, there is a population of Map2 + /IQGAP1 + /Iba1 + ( Figure 3H). Map2 + /IQGAP1 + /Iba1 + cells could represent cells undergoing a kind of EpithelialMesenchymal Transition (EMT) that lead to a dedifferentiate status mimicking macrophage-like cells [41,42]. In a mouse model of CNS metastasis, tumor cells behave like macrophages within the vasculature and during extravasation, expressing GIM/TAM markers, Iba1 among them [42,43].…”

Section: Iqgap1 In Neuronsmentioning

confidence: 99%

“…Map2 + /IQGAP1 + /Iba1 + cells could represent cells undergoing a kind of EpithelialMesenchymal Transition (EMT) that lead to a dedifferentiate status mimicking macrophage-like cells [41,42]. In a mouse model of CNS metastasis, tumor cells behave like macrophages within the vasculature and during extravasation, expressing GIM/TAM markers, Iba1 among them [42,43]. We assume that all neurons express IQGAP1 in a polarized manner, however, Map2 + /IQGAP1 − neurons, such as those shown in Supplementary Materials Figure S1, do not have IQGAP1 staining because it is probably not in that part of the stained histology slice, but we assume that IQGAP1 protein is expressed somewhere else in that polarized cell.…”

Section: Iqgap1 In Neuronsmentioning

confidence: 99%

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IQGAP1 in Podosomes/Invadosomes Is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

Rotoli

Pérez-Rodríguez

Morales

et al. 2017

Preprint

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Glioblastoma multiforme (GBM) is the most frequent and aggressive primary brain tumor. GBM is formed by a very heterogeneous astrocyte population, neurons, neovascularization and infiltrating myeloid cells (microglia and monocyte derived macrophages). The IQGAP1 scaffold protein interacts with components of the cytoskeleton, cell adhesion molecules, and several signaling molecules to regulate cell morphology and motility, cell cycle and other cellular functions. IQGAP1 overexpression and delocalization has been observed in several tumors, suggesting a role for this protein in cell proliferation, transformation and invasion. IQGAP1 has been identified as a marker of amplifying cancer cells in GBMs. To determine the involvement of IQGAP1 in the oncobiology of GBM, we performed immunohistochemical confocal microscopic analysis of the IQGAP1 protein in human GBM tissue samples using cell type-specific markers. IQGAP1 immunostaining and subcellular localization was heterogeneous; the protein was located in the plasma membrane and, at variable levels, in nucleus and/or cytosol. Moreover, IQGAP1 positive staining was found in podosome/invadopodia-like structures. IQGAP1 + staining was observed in neurons (Map2 + cells), in cancer stem cells (CSC; nestin + ) and in several macrophages (CD31 + or Iba1 + ). Our results indicate that the IQGAP1 protein is involved in normal cell physiology as well as oncologic processes.

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Section: Iqgap1 In Neuronsmentioning

confidence: 99%

Section: Iqgap1 In Neuronsmentioning

confidence: 99%

IQGAP1 in Podosomes/Invadosomes Is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

Rotoli

Pérez-Rodríguez

Morales

et al. 2017

Preprint

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show abstract

“…Most Map2 + cells, if not all, are IQGAP1 + stained ( Figure 3C,D,K-L and Supplemental Figure S1), in addition, there is a population of Map2 + /IQGAP1 + /Iba1 + ( Figure 3H). Map2 + /IQGAP1 + /Iba1 + cells could represent cells undergoing a kind of Epithelial -Mesenchymal Transition (EMT) that lead to a dedifferentiate status mimicking macrophage-like cells [41,42]. In a mouse model of CNS metastasis, tumor cells behave like macrophages within the vasculature and during extravasation, expressing GIM/TAM markers, Iba1 among them [42,43].…”

Section: Iqgap1 In Gbm Astrocytes and Gscsmentioning

confidence: 99%

“…Map2 + /IQGAP1 + /Iba1 + cells could represent cells undergoing a kind of Epithelial -Mesenchymal Transition (EMT) that lead to a dedifferentiate status mimicking macrophage-like cells [41,42]. In a mouse model of CNS metastasis, tumor cells behave like macrophages within the vasculature and during extravasation, expressing GIM/TAM markers, Iba1 among them [42,43]. We assume that all neurons express IQGAP1 in a polarized manner, however, Map2 + /IQGAP1 − neurons, as those shown in Supplemental Figure S1, do not have IQGAP1 staining because probably it is not in that part of the stained histology slice, but we assume that IQGAP1 protein is expressed somewhere else in that polarized cell.…”

Section: Iqgap1 In Gbm Astrocytes and Gscsmentioning

confidence: 99%

IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

Rotoli¹,

Pérez-Rodríguez²,

Morales³

et al. 2017

Preprint

View full text Add to dashboard Cite

Glioblastoma multiforme (GBM) is the most frequent and aggressive primary brain tumor. GBM is formed by a very heterogeneous astrocyte population, neurons, neovascularization and infiltrating myeloid cells (microglia and monocyte derived macrophages). The IQGAP1 scaffold protein interacts with components of the cytoskeleton, cell adhesion molecules, and several signalling molecules to regulate cell morphology and motility, cell cycle and other cellular functions. IQGAP1 overexpression and delocalization has been observed in several tumours, suggesting a role for this protein in cell proliferation, transformation and invasion. IQGAP1 has been identified as a marker of amplifying cancer cells in GBMs. To determine the involvement of IQGAP1 in the onco-biology of GBM, we performed immunohistochemical confocal microscopical analysis of the IQGAP1 protein in human GBM tissue samples using cell type-specific markers. IQGAP1 immunostaining and subcellular localization was heterogeneous; the protein was located in the plasma membrane and, at variable levels, in nucleus and/or cytosol. Moreover, IQGAP1 positive staining was found in podosome/invadopodia-like structures. IQGAP1 + staining was observed in neurons (Map2 + cells), in cancer stem cells (CSC; nestin + ) and in several macrophages (CD31 + or Iba1 + ). Our results indicate that the IQGAP1 protein is involved in normal cell physiology and also in oncologic processes.

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“…Beyin metastazlı hastalarda sağkalım yaş, hasta performansı, sistemik hastalığın varlığı ve yaygınlığı, mevcut metastazların sayısı ile ilişkili olmakla birlikte medyan sağkalım 2-15 ay arasında değişmektedir (1,2). En sık metastaz nedeni akciğer (%40-50) ve meme (%20-30) kanseridir ve beyin metastazlı olguların %70-80'inde 1-3 adet metastaz (oligometastaz) mevcuttur (3,4).…”

Section: Introductionunclassified

Tek Beyi̇n Metastazli Hastalarda Tüm Beyi̇n Ve Si̇mültane Entegre Boost Radyoterapi̇si̇ / Whole Brain Radiotherapy (Wbrt) With Simultaneous Integrated Boost (Sib) in Patients With Single Brain Metastasis

Özen

Şaylısoy

Metcalfe

et al. 2016

Osmangazi̇ Journal of Medicine

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ÖZET: Bu çalışmanın amacı tek beyin metastazına sahip küçük hücreli dışı akciğer kanseri (KHDAK) tanılı hastalarda yoğunluk ayarlı radyoterapi (YART) planlama yöntemi ile tüm beyin radyoterapisi + simültane entegre boost (TBRT + SEB) tekniğini kullanarak hedef dokuda doz yükseltilmesinin erken dönem sonuçlarının değerlendirmesidir. Tek beyin metastazına sahip ve TBRT + SEB tedavisi uygulanan KHDAK tanılı hastalar geriye dönük olarak değerlendirildi. Temmuz 2015 -Ocak 2016 arasında toplamda 11 hastaya ulaşıldı. Hasta ve tümöre ait özellikler, demografik veriler, tedavi bilgisi ve özellikleri incelendi. Tedavi süresince tüm hastalara antiödem tedavi rutin başlandı. Hastalarda grade III-IV toksisite gözlemlenmedi. Hastaların radyoterapiden 1 ay sonra çekilen beyin MR görüntüleri değerlendirildi ve tedavi öncesi beyin MR görüntüleri ile karşılaştırılarak 7 (%63) hastada tam yanıt, 4 (%37) hastada ise kısmi yanıt elde tespit edildi. Sonuç olarak TBRT + SEB tekniği radyocerrahi uygulamasına göre teknik olarak daha basit uygulanabilir, oldukça etkin ve hastalar tarafından tolere edilebilir bir yöntemdir. ANAHTAR KELİMELER: Tüm beyin radyoterapisi (TBRT), Simültane entegre boost (SEB), akciğer kanseri, tek beyin metastazı WHOLE BRAIN RADIOTHERAPY (WBRT) WITH SIMULTANEOUS INTEGRATED BOOST (SIB) IN PATIENTS WITH SINGLE BRAIN METASTASISABSTRACT: The aim of this study is to evaluate the early phase treatment results of dose escalation using by whole brain radiotherapy (WBRT) with simultaneous integrated boost (SIB) in non-small cell lung cancer patients with single brain metastasis. We evaluated retrospectively non-small cell lung cancer patients treated with WBRT + SIB. We achieved the data of 11 non-small cell lung cancer patients who were treated between July 2015 and January 2016. Patient and tumor characteristics, demographic data and treatment features have been evaluated. We prescribed antiedema treatment to all patients in whole duration of radiotherapy. We did not observe any grade III-IV toxicity. Cranial MRI images in the first month after radiotherapy has been evaluated and compared with preradiotherapy images. Complete response was identified in 7 (63%) patients and partial response was identified in 4 (37%) patients. In conclusion, WBRT + SIB technique is easier to apply when compared with radiosurgery, an effective and tolerable method for patients. KEYWORDS: Whole brain radiotherapy (WBRT), simultaneous integrated boost (SIB), lung cancer, single brain metastasis GirişBeyin metastazı tüm kanser olgularında %10-40 oranında görülmekte olup gelişen görüntüleme yöntemleri ile erken tanı olasılığı artmakta ve multidisipliner yaklaşımlar ile daha iyi sağkalım sonuçları elde edilmektedir (1). Beyin metastazlı hastalarda sağkalım yaş, hasta performansı, sistemik hastalığın varlığı ve yaygınlığı, mevcut metastazların sayısı ile ilişkili olmakla birlikte medyan sağkalım 2-15 ay arasında değişmektedir (1,2). En sık metastaz nedeni akciğer (%40-50) ve meme (%20-30) kanseridir ve beyin metastazlı olguların %70-80'inde 1-...

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The Molecular Biology of Brain Metastasis

Cited by 52 publications

References 106 publications

IQGAP1 in Podosomes/Invadosomes Is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

IQGAP1 in Podosomes/Invadosomes Is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

IQGAP1 in Podosome /Invadosome is Involved in the Progression of Glioblastoma Multiforme Depending on the Tumor Status

Tek Beyi̇n Metastazli Hastalarda Tüm Beyi̇n Ve Si̇mültane Entegre Boost Radyoterapi̇si̇ / Whole Brain Radiotherapy (Wbrt) With Simultaneous Integrated Boost (Sib) in Patients With Single Brain Metastasis

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