The molecular basis of O2-sensing and hypoxia tolerance in pheochromocytoma cells

Conrad, P. William; Conforti, Laura; Kobayashi, S; Beitner‐Johnson, Dana; Rust, R.T; Yang, Yuan; Kim, H W; Kim, R H; Seta, Karen A.; Millhorn, David E.

doi:10.1016/s1096-4959(00)00326-2

Cited by 33 publications

(24 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cellular responses to hypoxia have been extensively studied by using dopamine-containing PC12 cells (29). These cells can be efficiently infected with adenovirus particles to induce gene expression.…”

Section: Resultsmentioning

confidence: 99%

Methionine sulfoxide reductase A protects neuronal cells against brief hypoxia/reoxygenation

Yermolaieva

Schinstock

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

151

121

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Methionine sulfoxide reductase A protects neuronal cells against brief hypoxia/reoxygenation

Yermolaieva

Schinstock

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

151

121

View full text Add to dashboard Cite

“…Other genes associated with CR have functions suggesting they play a role in survival of sperm in vivo after environmental insults or in vitro following semen processing and cryopreservation. For example, AVP is antiapoptotic [29,30], and EPAS1 encodes for a transcription factor induced by hypoxia [31]. Furthermore, SERPINE2 can prevent capacitation [32] and may act to prevent premature capacitation.…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms in Candidate Genes Associated with Fertilizing Ability of Sperm and Subsequent Embryonic Development in Cattle1

Cochran

Cole

Null

et al. 2013

Biology of Reproduction

View full text Add to dashboard Cite

Fertilization and development of the preimplantation embryo is under genetic control. The present goal was to test 434 single nucleotide polymorphisms (SNPs) for association with genetic variation in fertilization and early embryonic development. The approach was to produce embryos from 93 bulls using in vitro procedures (n = 3-6 replicates per bull) and relate cleavage rate (CR) and development of cleaved embryos to the blastocyst stage (BDRC) to the genotype for each SNP. Bulls were selected to have either high or low estimates for predicted transmitted ability for daughter pregnancy rate (DPR), an estimate of female fertility. The repeatability was 0.84 for CR and 0.55 for BDRC. Semen extender affected CR, with lower results for milk extender than yolk extender. There was no significant correlation between DPR and either CR or BDRC. A total of 100 SNPs had a minor allele frequency sufficiently high (>5%) to allow association analysis. There were nine genes with SNPs associated with CR (AVP, DEPP, EPAS1, HSD17B6, NT5E, SERPINE2, SLC18A2, TBC1D24, and a noncharacterized gene) and 12 genes with SNPs associated with BDRC (C1QB, FAM5C, HSPA1A, IRF9, MON1B, PARM1, PCCB, PMM2, SLC18A2, TBC1D24, TTLL3, and WBP1). Results demonstrate that in vitro fertilization and blastocyst development are under genetic control and point out the potential importance of some previously unknown genes in these processes. Selection of cattle based on the genotype at one or more of these 19 loci may prove useful in conjunction with other genetic markers for improving genetic ability for fertility.

show abstract

“…Adenosine is an endogenous metabolite of ATP that has been proposed to have a protective func-P. W. Conrad et al / Comparative Biochcmistiy and Pliy.siolog\-Part B 128 (2001) [187][188][189][190][191][192][193][194][195][196][197][198][199][200][201][202][203][204] tion in neurons (Scanziani et al, 1992;Lupica et al, 1992). Adenosine is released in the central nervous system in response to ischemia and hypoxia, where it acts to decrease pre-and post-synaptic excitability, thereby protecting neurons against the metabolic stress associated with O, deprivation (Scanziani et al, 1992;Lupica et al^ 1992).…”

Section: Hypoxia Modulates the Adenosine Systemmentioning

confidence: 99%

“…This interaction then Induces the target genes, including the glucose transporter and glycolytic enzymes. Part B 128 (2001) [187][188][189][190][191][192][193][194][195][196][197][198][199][200][201][202][203][204] we hypothesized that calmodulin inhibitors would also block EPASl activation. Fig.…”

Section: Hsp32 (Ho-1)mentioning

confidence: 99%

Signal Transduction and Gene Regulation During Hypoxia Stress: A Potential Role in Neurodegenerative Disease

Millhorn¹

2002

Self Cite

View full text Add to dashboard Cite

Public reporting burden for this collection of infomiation is estimated to average 1 hour per response, including the time for reviewing instnjctions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway. Suite 1204, Arlington, VA 22202-4302, and PERFORMING ORGANIZATION NAIVIE(S) AND ADDRESS(ES)University of Cincinnati Cincinnati, Ohio 45267-0553 E-Mail: david.millhorn@uc.edu Soldiers deployed to high altitude terrain or exposed to chemical toxins that induce ischemia or impaired oxidative metabolism in the central nervous system (CNS) encounter sustained cellular hypoxia. This can compromise CNS function and lead to permanent neuronal injury, which is a precursor for neurodegenerative disorders such as Alzheimer's disease. The proposed research is designed to determine the role of stress-activated signal transduction systems in regulating a cellular phenotype that is tolerant to hypoxic stress. We hypothesize that de novo gene expression is a major component of the adaptative/protective response to hypoxia, and that the p38 kinase stress-activated pathway plays a major role in this response. We present novel preliminary findings, which show that genes involved in cell proliferation and differentiation are regulated by hypoxia and p38. We hypothesize that these genes and the genes that encode immediate early transcription factors, and the hypoxia-sensitive potassium channels are regulated by p38 during hypoxia and play a major role in protecting neurons form hypoxia injury and neurodegenerative disease. Studies are performed in PCI2 cells, which are extremely tolerant to reduced oxygen and a widely used model for elucidating the molecular mechanisms of neural function. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES)UThe objectives of the proposed research are: 1) Identify the p38 isoforms that are activated by hypoxia. Determine the effects of hypoxia on the protein kinases and small G-proteins that lie upstream of p38. 2) Determine the role of the p38 kinase pathway on the unique hypoxia-induced regulation of cyclin A, and immediate early genes in thsfos and jun families. 3) Determine the role of the p38 kinase pathway in regulating the hypoxia-induced expression of the oxygen-sensitive Kvl.2 potassium channel.14. SUBJECT TERMS hypoxia, p38 kinase pathways, cyclin A, immediate early genes, potassium channels, signal transduction, pheochromocytoma cells, neurodegenerative disease References 10Appendices 10 IntroductionThe research in this project was undertaken to determine the role of stress-activated signal transduction systems in regulating a cellular phenotype that can survive and function during hypoxic stress. The general hypothesis is that reduced 02 c...

show abstract

The molecular basis of O2-sensing and hypoxia tolerance in pheochromocytoma cells

Cited by 33 publications

References 55 publications

Methionine sulfoxide reductase A protects neuronal cells against brief hypoxia/reoxygenation

Methionine sulfoxide reductase A protects neuronal cells against brief hypoxia/reoxygenation

Single Nucleotide Polymorphisms in Candidate Genes Associated with Fertilizing Ability of Sperm and Subsequent Embryonic Development in Cattle1

Signal Transduction and Gene Regulation During Hypoxia Stress: A Potential Role in Neurodegenerative Disease

Contact Info

Product

Resources

About