The molecular basis of cryosurgery

Baust, John G.; Gage, Andrew A.

doi:10.1111/j.1464-410x.2005.05502.x

Cited by 317 publications

(207 citation statements)

References 24 publications

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“…During thawing, the intracellular compartment shifts to hypertonia, attracting fluid that causes the cell to burst. Mechanisms not at work in our model presented here are direct cold-induced coagulative necrosis that is the result of sublethal temperatures that activate apoptosis (Baust & Gage, 2005) and direct, cold-induced coagulative necrosis from vascular injury as a result of stasis, thrombosis, and ischemia. An interesting clinical effect is an intense immunogenicity after cryoablation that is different from heat coagulation as immunogenic epitopes are preserved (Jansen et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model

Dang

Waxman

Wang

et al. 2017

Preprint

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Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model Materials and Methods:We obtained 24 porcine eyes from a local abattoir, dissected and mounted them in an anterior segment perfusion and pressure transduction system within two hours of sacrifice. After they stabilized for 72 hours, eight eyes each were assigned to freeze-thaw (F) ablation (-80°C×2), to 0.02% saponin (S) treatment, or the control group (C), respectively. The trabecular meshwork was transduced with an eGFP expressing feline immunodeficiency viral (FIV) vector and tracked via fluorescent microscopy to confirm ablation. Following treatment, the eyes were perfused with standard tissue culture medium for 180 hours. We assessed histological changes by hematoxylin and eosin staining. TM cell viability was evaluated with a calcein AM/propidium iodide (PI) assay. We measured IOP and modeled it with a linear mixed effects model using a B-spline function of time with 5 degrees of freedom.Results: F and S experienced a similar IOP reduction by 30% from baseline (P =0.64). IOP reduction of about 30% occurred in F within 24 hours and in S within 48 hours. Live visualization of eGFP demonstrated that F conferred a complete ablation of all TM cells and only a partial ablation in S. Histological analysis confirmed that no TM cells survived in F while the extracellular matrix remained. The viability assay showed very low PI and no calcein staining in F in contrast to numerous PI-labeled dead TM cells and calcein-labeled viable TM cells in S. Conclusion:We developed a rapid TM ablation method that uses cyclic freezing that is free of biological or chemical agents and able to produce a decellularized TM scaffold with preserved TM extracellular matrix in an organotypic perfusion culture.

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Section: Discussionmentioning

confidence: 99%

Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model

Dang

Waxman

Wang

et al. 2017

Preprint

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“…5,8 This procedure utilizes the destructive forces of low temperature to ablate unwanted tissue. [9][10][11][12] The center (nearest the cryoprobe) of the cryogenic legion is rendered completely necrotic, but as temperatures elevate further from the probe tip, less is known about the mechanisms of cell death and survival. [13][14][15][16] In 1998, Hollister et al demonstrated that DNA from CaP cells frozen to temperatures of À5 to À151C exhibited a non-random, degradation pattern that is typically associated with late-stage apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Targeted induction of apoptosis via TRAIL and cryoablation: a novel strategy for the treatment of prostate cancer

Clarke

Robilotto

VanBuskirk

et al. 2007

Prostate Cancer Prostatic Dis

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Adjuvant therapies contribute to the successful treatment of cancer. Our previous reports have shown that combining cryoablation with cytotoxic agents enhances cell death. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytotoxic agent that preferentially induces apoptosis in a variety of human cancer cells. Human prostate cancer cells (PC-3) are resistant to many cytodestructive agents, including cryoablation and TRAIL. Here, we evaluated the effects of TRAIL combined with cryoablation on PC-3 and normal prostate (RWPE-1) cell death. Exposure of PC-3 cells to freezing (À101C) or TRAIL (500ng/ml) results in minimal cell death, whereas a complete loss of viability is observed with the simultaneous combination. The synergistic effect was found to be due to a marked increase in apoptosis. Western blot analysis revealed a significant level of caspase-8 and -3 cleavage between 12 and 24 h post-exposure. Caspase activation assays provided similar results and also indicated a role for caspase-9. Inhibitors to caspase-8 and -9 along with a pan-caspase inhibitor were incorporated to determine which pathway was necessary for the combined efficacy. Inhibition of caspase-8 significantly blocked the combination-induced cell death compared to cells that did not receive the inhibitor (63% compared to 10% viable). The addition of the caspase-9 inhibitor resulted in only a minimal protection. Importantly, the combination was not effective when applied to normal prostate cells. The results describe a novel therapeutic model for the treatment of prostate cancer and provide support for future in vivo studies.

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“…Geç dönemde, vasküler sistemde koagülasyon nekrozu meydana gelmekte ve endoteliyal hücre hasarına bağlı olarak gelişen yaygın tromboz, dokuda mikrosirkülasyonun bozulmasına yol açarak iskemik değişikliklere yol açmaktadır (8)(9)(10). Bu mekanizmalara ek olarak, apopitozis'in ve doku hasarı sırasında ortaya çıkan antijenlerin immün sistemi aktive etmesinin (immünmodülasyon) geç dönem doku hasarında rol oynadığını bildiren yayınlar mevcuttur (11,12).…”

Section: Etki Mekanizmasıunclassified

Küçük Böbrek Kitlelerinin Minimal İnvaziv Tedavisinde Kriyoablasyon mu, Radyofrekans Ablasyon mu?

Tuncel¹,

Senel²,

Aslan³

2015

Endouroloji

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The molecular basis of cryosurgery

Cited by 317 publications

References 24 publications

Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model

Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model

Targeted induction of apoptosis via TRAIL and cryoablation: a novel strategy for the treatment of prostate cancer

Küçük Böbrek Kitlelerinin Minimal İnvaziv Tedavisinde Kriyoablasyon mu, Radyofrekans Ablasyon mu?

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