The modulation of tissue-specific gene expression in rat nasal chondrocyte cultures by bioactive glasses

Asselin, Audrey

doi:10.1016/j.biomaterials.2004.01.016

Cited by 35 publications

(16 citation statements)

References 56 publications

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“…There is remarkable evidence of silicon's effect in bone formation (Asselin et al, ; Hench, Splinter, Allen, & Greenlee, ; Ilyas, Lavrik, Kim, Aswath, & Varanasi, ; Lu, Marcucio, & Miclau, ; Odatsu et al, ; Renno et al, ; Ryter et al, ; Tabak et al, ; Varanasi et al, ; D. Wang et al, ). Silicon‐based and Si‐doped materials have been used as biomaterials to enhance bone regeneration, as they elicit mineral deposition and osteoblast differentiation (Ilyas et al, ; Odatsu et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Silicon‐based and Si‐doped materials have been used as biomaterials to enhance bone regeneration, as they elicit mineral deposition and osteoblast differentiation (Ilyas et al, ; Odatsu et al, ). Currently, the biomaterials have been designed to act at a molecular level by stimulating a specific cellular response (Asselin et al, ). Since the 1970s, with the discovery of bioactive glass 45S5 (Bioglass®; Hench et al, ), these Si‐based materials have been shown to enhance bone healing and material‐bone attachment through Si 4+ and Ca 2+ release, inducing deposition of a hydroxycarbonate apatite layer within a few hours (Renno et al, ), posterior osteoblast differentiation, and collagen type I deposition (Varanasi et al, ; D. Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…There is remarkable evidence of silicon's effect in bone formation (Asselin et al, 2004;Hench, Splinter, Allen, & Greenlee, 1971;Ilyas, Lavrik, Kim, Aswath, & Varanasi, 2015;Lu, Marcucio, & Miclau, 2006;Odatsu et al, 2015;Renno et al, 2013;Ryter et al, 2007;Tabak et al, 2011;Varanasi et al, 2012;D. Wang et al, 2014).…”

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confidence: 99%

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Ionic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes

Monte

Cebe

Ripperger

et al. 2018

J Tissue Eng Regen Med

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Oxidative stress, induced by harmful levels of reactive oxygen species, is a common occurrence that impairs proper bone defect vascular healing through the impairment of endothelial cell function. Ionic silicon released from silica-based biomaterials, can upregulate hypoxia-inducible factor-1α (HIF-1α). Yet it is unclear whether ionic Si can restore endothelial cell function under oxidative stress conditions. Therefore, we hypothesized that ionic silicon can help improve human umbilical vein endothelial cells' (HUVECs') survival under toxic oxidative stress. In this study, we evaluated the ionic jsilicon effect on HUVECs viability, proliferation, migration, gene expression, and capillary tube formation under normal conditions and under harmful hydrogen peroxide levels. We demonstrated that 0.5-mM Si significantly enhanced angiogenesis in HUVECs under normal condition (p < 0.05). HUVECs exposed to 0.5-mM Si presented a morphological change, even without the bed of Matrigel, and formed significantly more tube-like structures than the control (p < 0.001). In addition, 0.5-mM Si enhanced cell viability in HUVECs under harmful H O levels. HIF-1α, vascular endothelial growth factor-A, and vascular endothelial growth factor receptor-2 were overexpressed more than twofold in silicon-treated HUVECs, under normal and toxic H O conditions. Moreover, the HUVECs were treated with 0.5-mM Si overexpressed superoxide dismutase-1 (SOD-1), catalase-1 (Cat-1), and nitric oxide synthase-3 (NOS3) under normal and oxidative stress environment (p < 0.01). A computational model was used for explaining the antioxidant effect of Si in endothelial cells and human periosteum cells by SOD-1 enhancement. In conclusion, we demonstrated that 0.5-mM Si can recover the HUVECs' viability under oxidative stress conditions by reducing cell death and upregulating expression of angiogenic and antioxidant factors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ionic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes

Monte

Cebe

Ripperger

et al. 2018

J Tissue Eng Regen Med

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show abstract

“…Asselin et al suggested that the calcium ion released from Bioglass ® has a stimulatory effect on the activation of osteoblasts [12]. We have focused on vaterite, which is one of bioresorbable polymorph of calcium carbonates.…”

Section: Introductionmentioning

confidence: 99%

Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium

et al. 2013

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Poly(l-lactic acid)-based films which include 60 wt % of vaterite (V) or siloxane-containing vaterite (SiV) were coated on a pure magnesium substrate, denoted by PLLA/V or PLLA/SiV, respectively, to suppress early corrosion and improve its cytocompatibility. Both coating films adhered to the Mg substrate with 2.3–2.8 MPa of tensile bonding strength. Soaking test for 7 days in α-modified minimum essential medium revealed that the morphological instability of the PLLA/V film caused a higher amount of Mg2+ ion to be released from the coating sample. On the other hand, in the case of the coating with the PLLA/SiV film, no morphological change even after the soaking test was observed, owing to the suppression of the degradation rate. In cell culture tests, the proliferation of mouse osteoblast-like cell (MC3T3-E1) was significantly enhanced by both coatings, in comparison with the uncoated magnesium substrate. The cell morphology revealed that a few less-spread cells were observed on the PLLA/V film, while more elongated cells were done on the PLLA/SiV film. The cells on the PLLA/SiV film exhibited an extremely higher alkaline phosphatase activity after 21 days of incubation than that on the PLLA/V one. The PLLA/SiV film suppressed the early corrosion and enhanced cytocompatibility on metallic magnesium.

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“…The granules of bioactive glasses containing silica were reported to enhance the expression of chondrogenic markers such as Sox9 14 . The cartilage formation may be enhanced by ionic silicon species.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Siloxane-Containing Vaterite ⁄ Poly (Lactic Acid) Hybrid Beads by Electrospraying and Ha-Coating on Their Surfaces

Nakamura

Obata

Kasuga

2010

Phosphorus Research Bulletin

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Bead-shaped siloxane-containing vaterite (SiV) / poly (L-lactic acid) (PLLA) hybrid beads with ca. 40 -130 µm in diameter were prepared by an electrospraying method. The beads included pores of ~1 µm in diameter. SiV particles were embedded in the PLLA matrix. The beads containing 20 wt% of SiV showed the releasabilities of Si 4+ and Ca 2+ ions in a dilute acetic acid solution of pH 4. To improve cytocompatibility, the beads were coated successfully with a hydroxyapatite layer by soaking in simulated body fluid (SBF).

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The modulation of tissue-specific gene expression in rat nasal chondrocyte cultures by bioactive glasses

Cited by 35 publications

References 56 publications

Ionic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes

Ionic silicon improves endothelial cells' survival under toxic oxidative stress by overexpressing angiogenic markers and antioxidant enzymes

Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium

Preparation of Siloxane-Containing Vaterite ⁄ Poly (Lactic Acid) Hybrid Beads by Electrospraying and Ha-Coating on Their Surfaces

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