The modulation of platelet and endothelial cell adhesion to vascular graft materials by perlecan

Lord, Megan S.; Yu, Weiyun; Bai, Chenglian; Simmons, Anne; Poole-Warren, Laura A.; Whitelock, John M.

doi:10.1016/j.biomaterials.2009.05.063

Cited by 59 publications

(74 citation statements)

References 51 publications

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“…Others have correlated the QCM signals and morphological changes with microscopy techniques but this has been done with cells that were extracted from their nutritive environment (i.e., fixed) (Elsom et al, 2008;Lord et al, 2009;Marx et al, 2001;Modin et al, 2006;Redepenning et al, 1993). Simultaneous microscopy observations and QCM-D signal detection would therefore act as a direct confirmation of frequency and energy dissipation changes.…”

Section: Introductionmentioning

confidence: 98%

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Fatisson

Azari

Tufenkji

2011

Biosensors and Bioelectronics

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Quartz crystal microbalance with dissipation monitoring (QCM-D) is used for real-time in situ detection of cytoskeletal changes in live primary endothelial cells in response to different cytomorphic agents; namely, the surfactant Triton-X 100 (TX-100) and bacterial lipopolysaccharide (LPS). Reproducible dissipation versus frequency (Df) plots provide unique signatures of the interactions between endothelial cells and cytomorphic agents. While the QCM-D response for TX-100 can be described in two steps (changes in the osmotic pressure of the medium prior to observing the expected cell lysis), LPS results in a different single-phase signal. A complementary analysis is carried out to evaluate the possible competitive effects of TX-100 and LPS through the QCM-D response to BAEC stress by analyzing the Df plots obtained. Experiments with non-toxic components (fibronectin or serum) produce a different QCM-D response than that observed for the toxic chemicals, suggesting the use of Df plot signatures for the possible differentiation between cytotoxic or non-cytotoxic effects. Observations obtained by QCM-D signals are confirmed by conducting fluorescence microscopy at the same time. Our results show that a fast (few minutes) sensing response can be obtained in situ and in real-time. The conclusions from this study suggest that QCM-D can potentially be used in biodetection for applications in drug screening tests and diagnosis.

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Section: Introductionmentioning

confidence: 98%

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Fatisson

Azari

Tufenkji

2011

Biosensors and Bioelectronics

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“…http In addition to substrate topography, the biochemical cues presented on the BM also provide instructive signals to ECs. A number of biomolecules have been immobilized onto biomaterial surfaces to promote re-endothelialization [5][6][7], improve the blood compatibility [14,15] and inhibit SMC proliferation [16]. Heparin, the most widely used anticoagulant drug, has been immobilized on material surfaces to improve blood compatibility by increasing the affinity of antithrombin III to thrombin [14,17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Since the vascular endothelium prevents blood coagulation and SMC proliferation, rapid re-endothelialization is critical to the success of vascular stents or grafts [1]. Several researchers have successfully enhanced re-endothelialization via various surface modification approaches [2][3][4][5][6][7]. However, the clinical results have been unsatisfactory [8], which may be attributed to the ignorance of competitive growth of endothelial cells (ECs) over SMCs in vivo [9,10].…”

Section: Introductionmentioning

confidence: 99%

Cooperative control of blood compatibility and re-endothelialization by immobilized heparin and substrate topography

Ding

Yang

et al. 2015

Acta Biomaterialia

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“…During the development of vascular grafts, intracoronary stents, the total artificial heart, and other blood-contacting medical devices, many strategies have been proposed for preventing thrombosis, including delivery of anticoagulants, the attachment of endothelial cells, 17,90-99 attachment of heparin, 100 the creation of nonfouling surfaces, [101][102][103][104] and other approaches. The use of anticoagulants (e.g., heparin) in the systemic circulation can be used to prevent acute thrombosis, but may lead to hemorrhage over the long term, and are not included in this review.…”

Section: Strategies For Preventing Thrombosismentioning

confidence: 99%

Strategies and Processes to Decellularize and Recellularize Hearts to Generate Functional Organs and Reduce the Risk of Thrombosis

Momtahan

Sukavaneshvar²,

Roeder

et al. 2015

Tissue Engineering Part B: Reviews

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Heart failure is one of the leading causes of death in the United States. Current therapies, such as heart transplants and bioartificial hearts, are helpful, but not optimal. Decellularization of porcine whole hearts followed by recellularization with patient-specific human cells may provide the ultimate solution for patients with heart failure. Great progress has been made in the development of efficient processes for decellularization, and the design of automated bioreactors. Challenges remain in selecting and culturing cells, growing the cells on the decellularized scaffolds without contamination, characterizing the regenerated organs, and preventing thrombosis. Various strategies have been proposed to prevent thrombosis of blood-contacting devices, including reendothelization and the creation of nonfouling surfaces using surface modification technologies. This review discusses the progress and remaining challenges involved with recellularizing whole hearts, focusing on the prevention of thrombosis.

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The modulation of platelet and endothelial cell adhesion to vascular graft materials by perlecan

Cited by 59 publications

References 51 publications

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Cooperative control of blood compatibility and re-endothelialization by immobilized heparin and substrate topography

Strategies and Processes to Decellularize and Recellularize Hearts to Generate Functional Organs and Reduce the Risk of Thrombosis

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