The modulation of lipid bilayer fluidity by intrinsic polypeptides and proteins

Cornell, Bruce; Sacré, M.M.; Peel, William E.; Chapman, D.

doi:10.1016/0014-5793(78)80291-9

Cited by 40 publications

(22 citation statements)

References 22 publications

(32 reference statements)

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“…When we look at the plane of the lipid bilayer (see fig.6) it is immediately Fig.6. Simulated lipid-polypeptide arrays with lipid : polypeptide ratios of (a) 1O:l and (b) 3:l [37).…”

Section: Discussionmentioning

confidence: 99%

“…At such high protein or polypeptide contents, the microviscosity will be high. Mobility of the probe molecule will be expected to be considerably inhibited and multiple contacts of 220 lipid with protein (trapped lipid) will occur [37]. (3) In ESR experiments of rhodopsin [52] using spin-labelled molecules which are attached to the protein but penetrate and sense the boundary layer, little effect on the mobility of the probe is found.…”

Section: Discussionmentioning

confidence: 99%

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Intrinsic protein—lipid interactions

1979

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“…When we look at the plane of the lipid bilayer (see fig.6) it is immediately Fig.6. Simulated lipid-polypeptide arrays with lipid : polypeptide ratios of (a) 1O:l and (b) 3:l [37).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intrinsic protein—lipid interactions

1979

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“…An increase in the density of slow cooperative motions has been postulated upon the addition ofproteins to a lipid bilayer (6)(7)(8)(9). Long-range protein-protein interactions have been observed in lipid bilayers (10,11).…”

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confidence: 99%

“…These include 20 mol % [6, A comparison of the T-j1 versus SCD plots for deuterons near the lipid headgroup with those for the lipid chain shows that the slope for the deuterons in [2,2,2',2'-2H4JDML is about 5 times smaller than that for the perdeuterated chain (Fig. 5).…”

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confidence: 99%

Characterization of the transverse relaxation rates in lipid bilayers.

Watnick

Dea

Chan

1990

Proc. Natl. Acad. Sci. U.S.A.

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The 2H NMR transverse relaxation rates of a deuterated phospholipid bilayer reflect slow motions in the bilayer membrane. A study of dimyristoyl lecithin specifically deuterated at several positions of the hydrocarbon chains indicates that these motions are cooperative and are confined to the hydrocarbon chains of the lipid bilayer. However, lipid head group interactions do play an important role in modulating the properties of the cooperative fluctuations of the hydrocarbon chains (director fluctuations), as evidenced by the effects of various lipid additives on the 2H NMR transverse relaxation rates of the dimyristoyl lecithin bilayer. Because liposomal bilayers are curved structures, however, the presence of significant lateral diffusion may complicate the angular dependence of T-1. If the rate of lateral diffusion is comparable to T-j', an average over X is necessary. The limits of this averaging may be expressed as,8 ± A,/, where the magnitude of Ad will depend both on the rate of lateral diffusion ofthe lipid molecules within each monolayer as well as the radius of curvature of the liposome. In the limit that lateral diffusion is complete over the time scale of T-1, (sin2(2,3)) = 16/30, and the angular dependence of T,-1 vanishes. However, when the rate of lateral diffusion is rapid compared to T-1, the physics is modified (20), the effects of director fluctuations are masked, and no angular dependence of T2-1 on p is expected.In this paper, we use the angular dependence of T, 1 and the dependence of T-j1 on the order parameter to characterize the nature of slow motions and to determine the contribution of director fluctuations to T-1 in a dimyristoyl lecithin (DML) bilayer. The effects ofdistearoyl lecithin (DSL), myristic acid (MA), cholesterol, phytol, and chlorophyll a (Chl-a) on the T-1 of the DML bilayer are also determined. The structures of these lipids are shown in Fig. 1 DML deuterated at the 9 and 10 positions on both chains ([9,9',10,10'-2H4]DML) was synthesized as described previously (4). [(2,2,2',2')-2H4]DML was synthesized by attaching MA deuterated at the 2 position to a glycerophosphocholine Abbreviations: DML, dimyristoyl lecithin; DSL, distearoyl lecithin; MA, myristic acid; Chl-a, chlorophyll a; Ti1, spin-spin relaxation rate.

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“…However, there exists a point of view [ 12,13] that the above-mentioned data could be satisfactorily explained as a result of the lipid entrapment in the protein network of natural or reconstituted membrane followed by a decrease of the mobility of the lipid molecules [14][15][16][17]. According to this opinion the boundary lipid content (mol/mol protein) must decrease with decreasing of the protein concentration in membrane [ 1"I], while according to 'boundary lipid' conception it should be constant.…”

Section: Introductionmentioning

confidence: 99%