The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)

Liu, Conghui; Zhang, Tao; Wang, Lingling; Wang, Mengqiang; Wang, Weilin; Jia, Zhihao; Jiang, Shuai; Song, Linsheng

doi:10.1016/j.dci.2016.06.002

Cited by 17 publications

(4 citation statements)

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“…The massive production of reactive oxygen species (ROS) and reactive oxygen intermediates induced by stress leads to many pathological conditions, and antioxidant systems protect the organism from superoxide [ 62 ]. Four main antioxidant enzyme classes, namely, superoxide dismutase (SOD), catalase (CAT), peroxidase (PRX), and glutathione peroxidase (GPX), were found in P. canaliculata and showed elevated global expression in response to stress ( Supplementary Tables S3 and S4 ).…”

Section: Resultsmentioning

confidence: 99%

The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation

et al. 2018

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BackgroundThe golden apple snail (Pomacea canaliculata) is a freshwater snail listed among the top 100 worst invasive species worldwide and a noted agricultural and quarantine pest that causes great economic losses. It is characterized by fast growth, strong stress tolerance, a high reproduction rate, and adaptation to a broad range of environments.ResultsHere, we used long-read sequencing to produce a 440-Mb high-quality, chromosome-level assembly of the P. canaliculata genome. In total, 50 Mb (11.4%) repeat sequences and 21,533 gene models were identified in the genome. The major findings of this study include the recent explosion of DNA/hAT-Charlie transposable elements, the expansion of the P450 gene family, and the constitution of the cellular homeostasis system, which contributes to ecological plasticity in stress adaptation. In addition, the high transcriptional levels of perivitelline genes in the ovary and albumen gland promote the function of nutrient supply and defense ability in eggs. Furthermore, the gut metagenome also contains diverse genes for food digestion and xenobiotic degradation.ConclusionsThese findings collectively provide novel insights into the molecular mechanisms of the ecological plasticity and high invasiveness.

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Section: Resultsmentioning

confidence: 99%

The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation

et al. 2018

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“…On the other hand, evidence for immune stimulation induced by repeated challenge with natural pathogens is available in clams and oysters (49)(50)(51)(52)(53)(54)(55). In the mussel M. galloprovincialis, increased bactericidal activity was observed after in vivo and subsequent in vitro challenge with Vibrio anguillarum (26).…”

Section: Discussionmentioning

confidence: 99%

Shift in Immune Parameters After Repeated Exposure to Nanoplastics in the Marine Bivalve Mytilus

et al. 2020

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Bivalves are widespread in coastal environments subjected to a wide range of environmental fluctuations: however, the rapidly occurring changes due to several anthropogenic factors can represent a significant threat to bivalve immunity. The mussel Mytilus spp. has extremely powerful immune defenses toward different potential pathogens and contaminant stressors. In particular, the mussel immune system represents a significant target for different types of nanoparticles (NPs), including amino-modified nanopolystyrene (PS-NH 2) as a model of nanoplastics. In this work, the effects of repeated exposure to PS-NH 2 on immune responses of Mytilus galloprovincialis were investigated after a first exposure (10 µg/L; 24 h), followed by a resting period (72-h depuration) and a second exposure (10 µg/L; 24 h). Functional parameters were measured in hemocytes, serum, and whole hemolymph samples. In hemocytes, transcription of selected genes involved in proliferation/apoptosis and immune response was evaluated by qPCR. First exposure to PS-NH 2 significantly affected hemocyte mitochondrial and lysosomal parameters, serum lysozyme activity, and transcription of proliferation/apoptosis markers; significant upregulation of extrapallial protein precursor (EPp) and downregulation of lysozyme and mytilin B were observed. The results of functional hemocyte parameters indicate the occurrence of stress conditions that did not however result in changes in the overall bactericidal activity. After the second exposure, a shift in hemocyte subpopulations, together with reestablishment of basal functional parameters and of proliferation/apoptotic markers, was observed. Moreover, hemolymph bactericidal activity, as well as transcription of five out of six immune-related genes, all codifying for secreted proteins, was significantly increased. The results indicate an overall shift in immune parameters that may act as compensatory mechanisms to maintain immune homeostasis after a second encounter with PS-NH 2 .

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“…Cavortin, however, lacks superoxide dismutase activity (Scotti et al, 2007). It has thus mainly been studied for its putative role in host defense based on its binding to pathogen-associated molecular patterns (PAMPs) and its expression in response to viral, bacterial, and protozoan challenges (Gonzalez et al, 2005; Duperthuy et al, 2011; Green et al, 2014; Normand et al, 2014; Liu et al, 2016). Proteins with sequences deduced from cDNA and similar to that of cavortin or part of cavortin were also identified in the Sydney rock oyster, Saccostrea glomerata (Sg-EcSOD, Green et al, 2009) and the European flat oyster, Ostrea edulis (Oe-EcSOD, Morga et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Dominin and Segon Form Multiprotein Particles in the Plasma of Eastern Oysters (Crassostrea virginica) and Are Likely Involved in Shell Formation

2019

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Dominin and segon are two proteins purified and characterized from the plasma of eastern oysters Crassostrea virginica , making up about 70% of the total plasma proteins. Their proposed functions are in host defense based on their pathogen binding properties and in metal metabolism based on their metal binding abilities. In the present study, the two proteins were further studied for their native states in circulation and extrapallial fluid and their possible involvement in shell formation. Two-dimensional electrophoresis confirmed that the oyster plasma was dominated by a few major proteins and size exclusion chromatography indicated that these proteins were present in circulation in a morphologically homogenous form. Density gradient ultracentrifugation in Cesium Chloride isolated morphologically homogenous particles of about 25 nm in diameter from the plasma and extrapallial fluids. Polyacrylamide gel electrophoresis identified dominin, segon and an unidentified protein as the principal components of the particles and the three proteins likely formed a multiprotein complex that associated to form the particle. Additionally, three major proteins extracted from shell organic matrix were identified based on the apparent molecular weight in SDS-PAGE to correspond to the three major proteins of plasma and protein particles. Moreover, the hemocyte expression of dominin and segon genes measured by real-time RT-PCR increased significantly upon the initiation of shell repair and were significantly greater in younger oysters. These findings suggest that dominin and segon form protein particles by association with each other and perhaps some other major plasma proteins and play a significant role in oyster shell formation.

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The modulation of extracellular superoxide dismutase in the specifically enhanced cellular immune response against secondary challenge of Vibrio splendidus in Pacific oyster (Crassostrea gigas)

Cited by 17 publications

References 48 publications

The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation

The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation

Shift in Immune Parameters After Repeated Exposure to Nanoplastics in the Marine Bivalve Mytilus

Dominin and Segon Form Multiprotein Particles in the Plasma of Eastern Oysters (Crassostrea virginica) and Are Likely Involved in Shell Formation

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