The modified Si-Jun-Zi Decoction attenuates colon cancer liver metastasis by increasing macrophage cells

Zhou, Jin‐Yong; Chen, Min; Wu, Cunen; Zhuang, Yingping; Chen, Yugen; Liu, Shenlin

doi:10.1186/s12906-019-2498-4

Cited by 22 publications

(14 citation statements)

References 22 publications

(23 reference statements)

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“…reversal effect on both weight loss and the severity (or onset) of the diarrhea status (Figures 3 and 4). Si-Jun-Zi-Tang is a traditional herbal formula that is used in gastroenterology and is also frequently utilized as a complementary agent for oncologic treatment due to its multiple roles in preventing tumor recurrence and metastasis and improving the quality of life (Lu et al, 2018;Zhou et al, 2019). Although there might be specific differences between human and rodents, we speculate that Si-Jun-Zi-Tang might compensate for the deficiencies of Lglutamine in addressing the physical manifestations of intestinal mucositis following anticancer drug treatment considering its multiple components and multiple targeted effects and the clinical practice, which possibly resulted in the higher efficacy of G-SJZ in the current study.…”

Section: Discussionmentioning

confidence: 99%

Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model

Tan

Yang

et al. 2020

Front. Pharmacol.

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Intestinal mucositis is a common toxicity of many anti-neoplastic therapies that negatively influences health, the quality of life, economic outcomes, and even the success of cancer treatment. Unfortunately, there is presently no optimal medicine that is able to effectively manage this condition. L-glutamine is one of the most frequently used agent in practice among the limited treatment choices due to its safety and inexpensiveness despite there being a lack of evidence. Previous studies indicated that L-glutamine may alleviate mucositis and mucosal atrophy but failed to improve patients' macroscopic conditions, such as the occurrence of diarrhea. A compound glutamine capsule (G-SJZ), composed of L-glutamine and the traditional Chinese herbal formula Si-Jun-Zi-Tang, has been used in China for 23 years to treat many types of gastrointestinal diseases, including gastrointestinal reactions induced by radiotherapy and chemotherapy. However, the exact effect of G-SJZ on intestinal mucositis is unclear, and moreover, whether Lglutamine combined with Si-Jun-Zi-Tang is more effective than L-glutamine alone have not been studied. In the current study, we explored the effects of G-SJZ and L-glutamine in a mouse model of intestinal mucositis induced by 5-fluorouracil (5-Fu). The results revealed that pretreatment with G-SJZ ameliorated the physical manifestations of weight loss and the severity of diarrhea following continuous 5-Fu injections in mice. Likewise, the histopathological damage and the destruction of villus and crypt structures in the intestinal mucosa as well as the increase in circulating intestinal injury markers caused by 5-Fu were reversed with G-SJZ pretreatment. Furthermore, the protective effect of G-SJZ was accompanied by modulations in the immunohistochemical expression of tight junction proteins. Interestingly, although treatment with a dose of Lglutamine alone that was equivalent to the dose in G-SJZ also showed a protective effect, it did not appear to be as strong as treatment with G-SJZ. Si-Jun-Zi-Tang in G-SJZ may compensate for the deficiencies of L-glutamine in this model which seems not to be related

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Section: Discussionmentioning

confidence: 99%

Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model

Tan

Yang

et al. 2020

Front. Pharmacol.

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“…TCM has unique advantages in treating cancers because of its comprehensive pharmacological effects of multi-channel, multi-levels, and multi-targets. For example, Chinese medicine formula Tian Xian Liquid, inhibits tumor growth effectively [6] and also enhances immunity by promoting macrophages proliferation [7].…”

Section: Introductionmentioning

confidence: 99%

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Xu³

et al. 2020

BMC Complement Med Ther

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Background: Lung cancer has the highest morbidity and mortality in the world and novel treatment strategies are still needed. Haimufang decoction (HMF) is a patented clinical prescription of traditional Chinese medicine for lung cancer treatment. HMF is composed of four herbs and has been applied clinically in advanced cancer patients. However, its therapeutic mechanisms are still unclear. This study aims to elucidate the possible mechanisms of HMF for the treatment of lung cancer. Methods: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was applied for evaluating the proliferative effect of HMF in lung cancer cells and monocyte macrophage RAW264.7 cells. Flow cytometer was used to detect the effects of HMF on cell cycle and apoptosis, and western blotting was employed to explore the potential apoptotic mechanisms of HMF on lung cancer cells. For immunomodulatory effect, co-culture system was used to detect the activation of macrophage RAW264.7 cells when treated with HMF, and neutral red assay was used to measure the effect of HMF on the phagocytosis of the activated macrophages. Enzyme linked immunosorbent assay, flow cytometer, and immunofluorescence staining method were employed for the investigation on the underlying mechanisms of the immunomodulatory effect on RAW264.7 induced by HMF.

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“…12-4801-82) (both eBioscience; Thermo Fisher Scientific, Inc.) staining was for macrophages and double positive CD11b-APC and Ly-6G-FITC mAb (Biolegend, cat no. 127606) staining was for neutrophiles ( 9 ).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the anticarcinogenic effect of several traditional Chinese medicines has also been emphasized in colon cancer ( 7 , 8 ). The authors' previous study demonstrated that modified Si-Jun-Zi Decoction (SJZ), a Chinese medicinal formula, could inhibit colon cancer liver metastasis by increasing the content of macrophage cells ( 9 ). A second Chinese medicine, acorus calamus, or its bioactive phytochemical β-asarone, has been widely reported to possess antitumor and chemopreventive activities in multiple carcinomas, including lung cancer ( 10 ), gastric cancer ( 11 ) and glioma ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

Chen

Zhuang

et al. 2021

Oncol Lett

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Studies have revealed that β-asarone exerts a powerful inhibitory effect on the proliferation of human cancer cells. The authors' previous study demonstrated that β-asarone could induce LoVo colon cancer cell apoptosis in vitro and in vivo, indicating its anticancer properties. The present study aimed to determine the antineoplastic effect of β-asarone in HCT116 colon cancer cells. An in vitro proliferation assay using a real time cell analyzer demonstrated that β-asarone effectively decreased HCT116 cell proliferation in a dose-dependent manner. Bioinformatics analysis revealed that differentially expressed genes following β-asarone inhibition were involved in the 'cell cycle', 'cell division', 'cell proliferation' and 'apoptosis'. Subsequently, a xenograft assay evidenced the inhibitory effect of β-asarone on the growth of HCT116 tumors in vivo. Further detection of immune-associated cytokines and cells suggested that β-asarone might be involved in the antitumor immune response by stimulating granulocyte-colony stimulating factor and increasing the number of macrophage cells in the spleen. Additionally, a murine model of splenic-transplantation verified the strong suppressive role of β-asarone in colon cancer liver metastasis in vivo. Taken together, the results of the current study revealed that β-asarone decreased HCT116 colon cancer cell proliferation and liver metastasis potentially by activating the innate immune system, supporting the multi-system regulation theory and providing a basis for further mechanistic studies on colon cancer.

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The modified Si-Jun-Zi Decoction attenuates colon cancer liver metastasis by increasing macrophage cells

Cited by 22 publications

References 22 publications

Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model

Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

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