The modification of native and denatured keratin by alkali

Mellet, Philippe; Swanepoel, O.A.

doi:10.1007/bf00646578

Cited by 6 publications

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“…[7 ] , respectively, amino acid analyses were carried out according to established column chromatographic procedures [25,2,18] ] using a Beckman . automatic amino acid analyzer.…”

Section: Experimental and Resultsmentioning

confidence: 99%

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The Influence of Alkali Treatment on Native and Denatured Proteins

Mellet¹

1968

Textile Research Journal

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The influence of alkali treatment on wool keratin and on silk fibroin was investigated in a medium of K 2 CO 3 at 50° C. It was found that the denaturing action of LiBr affects the reactivity and reactions of the disulfides differently from stretching of the fibers does.Since the sum total of the sulfur-containing amino acids remains constant during alkali treatment of both native and denatured wool, the participation of cystine in the formation of lysinoalanine is excluded. It is suggested that seryl residues are responsible for the formation of α-amino acrylic acid residues which combine with the lysine present in wool or in silk to form lysinoalanine. The sulfur content in wool, as obtained by direct determination, was higher than the expected values, and this indicates the presence of sulfur compounds formed during the alkali treatment, which are not analyzed as any of the known sulfur-containing amino acids. No evidence was found that peptide-bound lanthionine is unstable towards 0.7 M K 2 CO 3 solution at 50°C. A significant increase in N-terminal seryl residues was observed for alkali-treated wool. Various possible explanations of this phenomenon were investigated. '

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“…[7 ] , respectively, amino acid analyses were carried out according to established column chromatographic procedures [25,2,18] ] using a Beckman . automatic amino acid analyzer.…”

Section: Experimental and Resultsmentioning

confidence: 99%

“…Parts of the results reported in this paper have been published as short communications[17,19] 2. Present address: Dept.…”

mentioning

confidence: 94%