The modification of gelatin films: Based on various cross‐linking mechanism of glutaraldehyde at acidic and alkaline conditions

Lin, Junjie; Pan, Daodong; Sun, Yangying; Ou, Changrong; Wang, Ying; Cao, Jinxuan

doi:10.1002/fsn3.1282

Cited by 52 publications

(44 citation statements)

References 25 publications

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“…The spectrum of the precursor and that of the 3D printed scaffold before incubation in SBF presented combined signals for the two organic and inorganic phases. A broad peak centered at about 3530–3290 cm −1 was assigned to the combined O–H and N–H vibrations of the protein matrix, and C–H peaks were noticed at about 2940–2870 cm −1 , with amide I and amide II at approximately 1680–1530 cm −1 (overlapping with water vibration), and a complex signal around 1060–1040 cm −1 similarly to other works [ 42 ]. The vibrations at 1017.27 and 962.305 cm −1 in the precursor and at 1012.45 and 963.269 cm −1 in the CS–FG3 scaffold correspond to Si–O; the peaks at 896.737 and 878.417 cm −1 in the precursor and at 878.417 and 864.917 cm −1 in the CS–FG3 scaffold are assigned to the stretching vibrations of Si–O–Si from the CS phase.…”

Section: Resultssupporting

confidence: 78%

Development of 3D Bioactive Scaffolds through 3D Printing Using Wollastonite–Gelatin Inks

et al. 2020

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The bioactivity of scaffolds represents a key property to facilitate the bone repair after orthopedic trauma. This study reports the development of biomimetic paste-type inks based on wollastonite (CS) and fish gelatin (FG) in a mass ratio similar to natural bone, as an appealing strategy to promote the mineralization during scaffold incubation in simulated body fluid (SBF). High-resolution 3D scaffolds were fabricated through 3D printing, and the homogeneous distribution of CS in the protein matrix was revealed by scanning electron microscopy/energy-dispersive X-ray diffraction analysis (SEM/EDX) micrographs. The bioactivity of the scaffold was suggested by an outstanding mineralization capacity revealed by the apatite layers deposited on the scaffold surface after immersion in SBF. The biocompatibility was demonstrated by cell proliferation established by MTT assay and fluorescence microscopy images and confirmed by SEM micrographs illustrating cell spreading. This work highlights the potential of the bicomponent inks to fabricate 3D bioactive scaffolds and predicts the osteogenic properties for bone regeneration applications.

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Section: Resultssupporting

confidence: 78%

Development of 3D Bioactive Scaffolds through 3D Printing Using Wollastonite–Gelatin Inks

et al. 2020

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“…First, a slight degradation step with onset at around 160 °C, except for M2 that declines smoothly and shows no inflection point in the first derivative plot ( Figure 2 b). Nevertheless, this first stage leads to a weight variation of around −10% for all samples due to loss of absorbed water [ 30 ]. Second, a much more pronounced decline in a mass close to 300 °C, carrying a 60% weight reduction.…”

Section: Resultsmentioning

confidence: 99%

Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities

Valcárcel¹,

Fraguas²,

Hermida-Merino

et al. 2021

Marine Drugs

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Rising trends in fish filleting are increasing the amount of processing by-products, such as skins of turbot, a flatfish of high commercial value. In line with circular economy principles, we propose the valorization of turbot skins through a two-step process: initial gelatin extraction described for the first time in turbot, followed by hydrolysis of the remaining solids to produce collagen hydrolysates. We assayed several methods for gelatin extraction, finding differences in gelatin properties depending on chemical treatment and temperature. Of all methods, the application of NaOH, sulfuric, and citric acids at 22 °C results in the highest gel strength (177 g), storage and loss moduli, and gel stability. We found no relation between mechanical properties and content of pyrrolidine amino acids, but the best performing gelatin displays higher structural integrity, with less than 30% of the material below 100 kDa. Collagen hydrolysis was more efficient with papain than alcalase, leading to a greater reduction in Mw of the hydrolysates, which contain a higher proportion of essential amino acids than gelatin and show high in vitro anti-hypertensive activity. These results highlight the suitability of turbot skin by-products as a source of gelatin and the potential of collagen hydrolysates as a functional food and feed ingredient.

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“…The TGA thermogram in Figure 2 a shows a profile of weight loss with increasing temperature that can be divided into two separate regions. First, a slight degradation step with onset at around 100 °C, this first stage leads to a weight variation of around −8%, due to loss of absorbed water ( Table S1, Supplementary Materials ) [ 36 ]. Second, a much more pronounced decline in mass close to 300 °C, carrying a 60% weight reduction.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Gelatin and Hydrolysates from Valorization of Farmed Salmon Skin By-Products

et al. 2021

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Salmon processing commonly involves the skinning of fish, generating by-products that need to be handled. Such skin residues may represent valuable raw materials from a valorization perspective, mainly due to their collagen content. With this approach, we propose in the present work the extraction of gelatin from farmed salmon and further valorization of the remaining residue through hydrolysis. Use of different chemical treatments prior to thermal extraction of gelatin results in a consistent yield of around 5%, but considerable differences in rheological properties. As expected from a cold-water species, salmon gelatin produces rather weak gels, ranging from 0 to 98 g Bloom. Nevertheless, the best performing gelatins show considerable structural integrity, assessed by gel permeation chromatography with light scattering detection for the first time on salmon gelatin. Finally, proteolysis of skin residues with Alcalase for 4 h maximizes digestibility and antihypertensive activity of the resulting hydrolysates, accompanied by the sharpest reduction in molecular weight and higher content of essential amino acids. These results indicate the possibility of tuning salmon gelatin properties through changes in chemical treatment conditions, and completing the valorization cycle through production of bioactive and nutritious hydrolysates.

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The modification of gelatin films: Based on various cross‐linking mechanism of glutaraldehyde at acidic and alkaline conditions

Cited by 52 publications

References 25 publications

Development of 3D Bioactive Scaffolds through 3D Printing Using Wollastonite–Gelatin Inks

Development of 3D Bioactive Scaffolds through 3D Printing Using Wollastonite–Gelatin Inks

Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities

Characterization of Gelatin and Hydrolysates from Valorization of Farmed Salmon Skin By-Products

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