The Modeling and Analysis of Mechanisms for lncRNA Regulation

N3‐Kethoxal‐Based Bioorthogonal Intracellular RNA Labeling

“…By solid‐phase chemical synthesis, ligation, aptamer‐ or enzyme‐based labeling, RNA can be labeled with radioactive or fluorescent groups, biotins and enzymes [7–10] . For instance, a 32 P radiolabel, with the advantages of increased sensitivity and easy disposal, has been widely used in synthesized RNAs for experimental analyses [8] . However, in vivo labeling techniques for RNA lag behind in vitro methods.…”

“…Research into RNA structure, detection or therapeutics has commonly been undertaken through in vitro RNA labeling methods. By solid‐phase chemical synthesis, ligation, aptamer‐ or enzyme‐based labeling, RNA can be labeled with radioactive or fluorescent groups, biotins and enzymes [7–10] . For instance, a 32 P radiolabel, with the advantages of increased sensitivity and easy disposal, has been widely used in synthesized RNAs for experimental analyses [8] .…”