The model of resting forms of micobacteria for testing of chemodrugs for latent forms of tuberculosis

Anuchin, A. M.; Гончаренко, А. В.; Galon, I. V.; Demidenok, O. I.; Kudykina, Yu. K.; Moisenovich, Mikhail M.; Mulyukin, A. L.; Kaprelyants, Arseny S.

doi:10.1134/s0003683810030063

Cited by 4 publications

(2 citation statements)

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“…It is important that these cells retained culturability, demonstrating reversibility of the toxin‐inducible phenotype. We have previously shown that under certain conditions, transition to dormancy resulted in formation of non‐replicating, but culturable ovoid forms of M. smegmatis (Anuchin et al ., , ) and of M. tuberculosis (Shleeva et al ., , ). Interestingly, formation of ‘lemon‐shaped’ E. coli cells was documented after overexpression of YeeV (Tan et al ., ) and CptA (YgfX; Masuda et al ., ), toxins which target essential cytoskeleton proteins, FtsZ and MreB.…”

Section: Discussionmentioning

confidence: 99%

“…Dormancy of mycobacteria is often accompanied by so-called 'nonculturability'a temporary loss of ability to grow on solid media (Shleeva et al, 2002;Mukamolova et al, 2003). However, mycobacteria in the dormant state that retain culturability have also been described (Anuchin et al, 2009(Anuchin et al, , 2010. It has been suggested that formation of the dormant state is controlled by a particular 'programme' and that TA systems probably participate in this process (Arcus et al, 2005;Condon, 2006;Zhu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Toxin-antitoxinvapBClocus participates in formation of the dormant state inMycobacterium smegmatis

Demidenok

Kaprelyants

Гончаренко

2014

FEMS Microbiol Lett

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Toxin-antitoxin (TA) loci are widely spread in bacterial plasmids and chromosomes. Toxins affect important functions of bacterial cells such as translation, replication and cell-wall synthesis, whereas antitoxins are toxin inhibitors. Participation in formation of the dormant state in bacteria is suggested to be a possible function of toxins. Here we show that overexpression of VapC toxin in Mycobacterium smegmatis results in development of morphologically distinct ovoid cells. The ovoid cells were nonreplicating and revealed a low level of uracil incorporation and respiration that indicated their dormant status. To validate the role of VapBC in dormancy formation, we used a model of dormant, 'nonculturable' (NC) M. smegmatis cells obtained in potassium-limited conditions. Overexpression of VapB antitoxin prevented transition to dormancy, presumably due to a decreased level of the free VapC protein. Indeed, this effect of the VapB was neutralized by coexpression of the cognate VapC as a part of the vapBC operon. In summary, these findings reveal participation of vapBC products in formation of the dormant state in M. smegmatis.

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