The mode of inheritance in tetraploid cut roses

Abstract

Tetraploid hybrid tea roses (Rosa hybrida) represent most of the commercial cultivars of cut roses and form the basis for breeding programmes. Due to intensive interspecific hybridizations, modern cut roses are complex tetraploids for which the mode of inheritance is not exactly known. The segregation patterns of molecular markers in a tetraploid mapping population of 184 genotypes, an F1 progeny from a cross of two heterozygous parents, were investigated for disomic and tetrasomic inheritance. The possible occurrence of double reduction was studied as well. We can exclude disomic inheritance, but while our observations are more in line with a tetrasomic inheritance, we cannot exclude that there is a mixture of both inheritance modes. Two novel parental tetraploid linkage maps were constructed using markers known from literature, combined with newly generated markers. Comparison with the integrated consensus diploid map (ICM) of Spiller et al. (Theor Appl Genet 122:489–500, 2010) allowed assigning numbers to each of the linkage groups of both maps and including small linkage groups. So far, the possibility of using marker-assisted selection in breeding of tetraploid cut roses and of other species with a tetrasomic or partly tetrasomic inheritance, is still limited due to the difficulties in establishing marker-trait associations. We used these tetraploid linkage maps to determine associations between markers, two morphological traits and powdery mildew resistance. The knowledge on inheritance and marker-trait associations in tetraploid cut roses will be of direct use to cut rose breeding.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-012-1855-1) contains supplementary material, which is available to authorized users.

The Double-Reduction Landscape in Tetraploid Potato as Revealed by a High-Density Linkage Map

“…This fact has already been reported (Qu and Hancock 2001;Koning-Boucoiran et al 2012) and forms the basis for a test of preferential pairing that we also exploit in this study. It is evident that preferential pairing can have a severe impact on the correct assignment of repulsion phase ( Figure 7A) regardless of whether MINR or MLL is used for phase assignment (data not shown).…”

Genetic mapping in polyploids

“…This fact has already been reported (Qu and Hancock, 2001;Koning-Boucoiran et al, 2012) and forms the basis for a test of preferential pairing which we also exploit in this study. It is evident that preferential pairing can have a severe impact on the correct assignment of repulsion phase (Figure 7.a), regardless of whether MINR or MLL is used for phase-assignment (data not shown).…”

“…Of some concern are polyploid species for which the mode of inheritance is neither strictly polysomic nor disomic, but something in between. There have been various reports of "segmental allopolyploidy" (Stebbins, 1947;Sybenga, 1996), for example in rose (Koning-Boucoiran et al, 2012), garden dahlia (Schie et al, 2014) and peanut (Leal-Bertioli et al, 2015). One of the advantages of our approach is that it predominantly relies on coupling-phase estimates which have been shown to be more robust against preferential pairing than repulsion-phase estimates (the case of SxN markers is covered in detail in ).…”

“…Rosa is generally divided into ten sections, four of which (Synstylae, Gallicanae, Indicae and Pimpinellifoliae) have contributed to the domesticated rose genepool . Rosa hybrida or the hybrid tea rose is nowadays the most well-known and commercially-important representative of the genus and has a complex mixture of hybrid perpetuals derived from China rose, Noisettes (Rosa chinensis), Bourbons as well as R. gallica and R. alba, and tea roses (R. x odorata) in its pedigree (Koning-Boucoiran et al, 2012;Liorzou et al, 2016). Given this complexity of origin, it is not surprising that it remains a poorly-understood species genetically (Debener and Linde, 2009).…”

“…In contrast, autotetraploid maps are far fewer, with the exception of alfalfa (Medicago sativa L.) (Brouwer and Osborn, 1999;Robins et al, 2008), potato (Solanum tuberosum L.) Hackett et al, 2013) and rose (Rosa hybrida) (Rajapakse et al, 2001;Koning-Boucoiran et al, 2012).…”