The MIXTA-like Transcription factor MYB16 is a major regulator of cuticle formation in vegetative organs

Oshima, Yoshimi; Mitsuda, Nobutaka

doi:10.4161/psb.26826

Cited by 59 publications

(55 citation statements)

References 27 publications

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“…The differentially expressed TFs included those with a possible link to the circadian clock, with two genes (Solyc01g095030 and Solyc10g084370) encoding MYB-related TFs of the REVEILLE family (Farinas and Mas, 2011), one of which was expressed at lower levels in gpat6-a, and six genes encoding CONSTANS-like TFs (Valverde, 2011), one of which (Solyc12g005660) was expressed at much lower levels. Despite the large number of differentially expressed TFs identified, the group did not include TFs that have been reported to regulate cuticle formation in tomato or other species (Shi et al, 2013;Borisjuk et al, 2014;Hen-Avivi et al, 2014), other than a MIXTA-like MYB TF (Oshima and Mitsuda, 2013), whose role in tomato was described recently (Lashbrooke et al, 2015). We observed that the expression of SlMIXTA-like (Solyc02g088190) was lower in the gpat6-a mutant than in the wild type (Supplemental Table S5).…”

Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness Andmentioning

confidence: 66%

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

et al. 2016

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The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micro-Tom and isolated several glossy fruit mutants in which the abundance of cutin, the polyester component of the cuticle, was strongly reduced. We employed a newly developed mapping-by-sequencing strategy to identify the causal mutation underlying the cutin deficiency in a mutant thereafter named gpat6-a (for glycerol-3-phosphate acyltransferase6). To this end, a backcross population (BC 1 F 2 ) segregating for the glossy trait was phenotyped. Individuals displaying either a wild-type or a glossy fruit trait were then pooled into bulked populations and submitted to whole-genome sequencing prior to mutation frequency analysis. This revealed that the causal point mutation in the gpat6-a mutant introduces a charged amino acid adjacent to the active site of a GPAT6 enzyme. We further showed that this mutation completely abolished the GPAT activity of the recombinant protein. The gpat6-a mutant showed perturbed pollen formation but, unlike a gpat6 mutant of Arabidopsis (Arabidopsis thaliana), was not male sterile. The most striking phenotype was observed in the mutant fruit, where cuticle thickness, composition, and properties were altered. RNA sequencing analysis highlighted the main processes and pathways that were affected by the mutation at the transcriptional level, which included those associated with lipid, secondary metabolite, and cell wall biosynthesis.

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Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness Andmentioning

confidence: 66%

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

et al. 2016

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“…MYB106 and MYB16 were found to participate in the regulation of cuticle biosynthesis through partially cooperated with WIN1/SHN1 [38]. MYB16 was showed act as a major regulator of cuticle formation by regulating the cuticle biosynthesis genes CYP86A8/LCR and CER1 [39]. These studies indicate that TFs, particularly MYBs, play a curial role for the regulation of cuticle formation by activating the expression of synthesis genes.…”

Section: Introductionmentioning

confidence: 80%

“…Double mutants of MYB33 with MYB65 are male sterile with small anthers and defective pollens [81]. Other MYBs, like MYB106, MYB30, MYB41, MYB16 and MYB96 are regulated cuticle formation in virous tissues by activating the biosynthesis genes of epidermal wax in Arabidopsis [34,37,39,82].…”

Section: Formation Of Anther Cuticle and Tryphine May Rely On The Regmentioning

confidence: 99%

Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line

Zhang

Liu

et al. 2020

Preprint

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Background: Male sterility is a simple and efficient pollination control system that is widely exploited in hybrid breeding. In upland cotton, CCRI9106, a photosensitive genetic male sterile (PGMS) mutant isolated from CCRI040029, was reported of great advantages to cotton heterosis. However, little information concerning the male sterility of CCRI9106 is known. Here, comparative transcriptome analysis of CCRI9106 (the mutant, MT) and CCRI040029 (the wild type, WT) anthers in Anyang (long-day, male sterile condition to CCRI9106) was performed to reveal the potential male sterile mechanism of CCRI9106.Results: Light and electron microscopy revealed that the male sterility phenotype of MT was mainly attributed to irregularly exine, lacking tryphine and immature anther cuticle. Based on the cytological characteristics of MT anthers, anther RNA libraries (18 in total) of tetrad (TTP), late uninucleate (LUNP) and binucleate (BNP) stages in MT and WT were constructed for transcriptomic analysis, therefore revealing a total of 870,4 differentially expressed genes (DEGs). By performing gene expression pattern analysis and protein-protein interaction (PPI) networks construction, we found down-regulation of DEGs, which enriched by the lipid biosynthetic process and the synthesis pathways of several types of secondary metabolites such as terpenoids, flavonoids and steroids, may crucial to the male sterility phenotype of MT, and resulting in the defects of anther cuticle and tryphine, even the irregularly exine. Furthermore, several lipid-related genes together with ABA-related genes and MYB transcription factors were identified as hub genes via weighted gene co-expression network analysis (WGCNA). Additionally, the ABA content of MT anthers was reduced across all stages when compared with WT anthers. At last, genes related to the formation of anther cuticle and tryphine could activated in MT under short-day condition.Conclusions: We propose that the down-regulation of genes related to the assembly of anther cuticle and tryphine may lead to the male sterile phenotype of MT, and MYB transcription factors together with ABA played key regulatory roles in these processes. The conversion of fertility in different photoperiods may closely relate to the functional expression of these genes. These findings contribute to elucidate the mechanism of male sterility in upland cotton.

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“…In vegetative organs, the null mutation of MYB106 does not lead to cuticular deficiencies except in trichomes. However, the double knockout/down of MYB106 and MYB16 results in an apparent leaf cuticle functional deficiency (Oshima and Mitsuda 2013b). Using these mutant lines, we showed that MYB106 and MYB16 are required for the formation of cuticle nanoridges in petals and stamens.…”

mentioning

confidence: 95%

“…We previously revealed that MYB106 and MYB16, which is the closest MYB106 paralog, regulate cuticle development in Arabidopsis thaliana and Torenia fournieri (Oshima et al 2013a;Oshima and Mitsuda 2013b). In vegetative organs, the null mutation of MYB106 does not lead to cuticular deficiencies except in trichomes.…”

mentioning

confidence: 99%

Enhanced cuticle accumulation by employing MIXTA-like transcription factors

Oshima

Mitsuda

2016

Plant Biotechnology

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The cuticle covers almost the entire aerial surface of terrestrial plants, and provides protection from abiotic and biotic stresses. Cuticles basically consist of wax and cutin, and are produced with variable structures and thicknesses depending on the plant and organ. The application of plant cuticles to improve stress tolerance and wax production requires the deposition of the cuticle at specific times to avoid undesirable side effects. We previously showed that the MYB106 and MYB16 MIXTA-like transcription factors regulate cuticle formation. However, MYB106 over-expression results in severe dwarfism. In this study, we identified genes downstream of these MYB transcription factors and used their promoters to express MYB106 and MYB16 fused to the strong transcriptional activation domain, VP16. Comparisons of plant growth and cuticle morphology revealed that MYB106 and MYB16 preferentially produced cuticles that are typically observed in petals and leaves, respectively. Additionally, the CYP77A6 and CYP86A4 promoters effectively induced cuticle accumulation in leaves and petals, respectively, without inhibiting plant growth. Our strategy may be useful for increasing or altering cuticles in agronomically important plants.

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The MIXTA-like Transcription factor MYB16 is a major regulator of cuticle formation in vegetative organs

Cited by 59 publications

References 27 publications

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line

Enhanced cuticle accumulation by employing MIXTA-like transcription factors

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