The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins

Kispál, Gyula; Csere, Péter; Prohl, Corinna; Lill, Roland

doi:10.1093/emboj/18.14.3981

Cited by 638 publications

(787 citation statements)

References 56 publications

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“…The activity of isopropylmalate isomerase can be detected by the formation of the UVabsorbing double bond of isopropylmaleate on dehydration of 3-isopropylmalate 10 . In a similar manner, formation of cis-aconitate from isocitrate in concentrated aconitase preparations (mitochondria) can be followed 10 . For diluted aconitase samples (whole-cell extract), this assay cannot be used due to the high UV absorbance from DNA and other proteins.…”

Section: Experimental Designmentioning

confidence: 99%

“…The yeast strain used should also be considered. High activity of isopropylmalate isomerase (Leu1) is particularly observed in the yeast W303 genetic background 10 . Effects by strain and medium differences are not confined to the yeast system.…”

Section: Experimental Designmentioning

confidence: 99%

“…50,51 Incorporation of radioactive iron into Fe/S proteins in vivo. The pulse radiolabeling of yeast cells with 55 Fe provides a faithful assay for the estimation of the de novo biogenesis of Fe/S cluster-containing proteins in vivo 10 . A similar approach has been employed to study Fe/S protein assembly in isolated or lysed mitochondria in vitro 52 .…”

Section: Experimental Designmentioning

confidence: 99%

“…Strikingly, maturation of the extra-mitochondrial Fe/S proteins depends on the function of the mitochondrial ISC assembly machinery 10 . A current working model ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of iron–sulfur protein maturation in eukaryotes

2009

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Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

“…Strikingly, maturation of the extra-mitochondrial Fe/S proteins depends on the function of the mitochondrial ISC assembly machinery 10 . A current working model ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of iron–sulfur protein maturation in eukaryotes

2009

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“…For ABC transporters such as Ste6p, Atmp1p, and Pxa1p/ Pxa2p, the physiological substrates have been identified [23][24][25]42,51]. As for PDR transporters, detoxification remains as one of the most plausible physiological function, since they General TF and other Ngg1p Inhibition of Pdr1p activity [66,67] PDR, pleiotropic drug resistance; TF, transcription factor.…”

Section: The Physiological Role Of Abc Proteins In S Cerevisiaementioning

confidence: 99%

Yeast ABC transporters – A tale of sex, stress, drugs and aging

2005

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Yeast ATP-binding cassette (ABC) proteins are implicated in many biological phenomena, often acting at crossroads of vital cellular processes. Their functions encompass peptide pheromone secretion, regulation of mitochondrial function, vacuolar detoxification, as well as pleiotropic drug resistance and stress adaptation. Because yeast harbors several homologues of mammalian ABC proteins with medical importance, understanding their molecular mechanisms, substrate interaction and three-dimensional structure of yeast ABC proteins might help identifying new approaches aimed at combating drug resistance or other ABC-mediated diseases. This review provides a comprehensive discussion on the functions of the ABC protein family in the yeast Saccharomyces cerevisiae.

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Proton-Coupled Electron Transfer from Sulfur: A S-H/S-D Kinetic Isotope Effect of ≥31.1

2000

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There is an extensive redox chemistry of high oxidation state ruthenium(iv) oxo, [1] and osmium(vi) nitrido, [2] and osmium(iv ± vi) hydrazido [3] complexes based on multiple electron/atom transfers such as O atom, [4] N À ion, [3] and H À [2]Cl 6´1 6 H 2 O: To a solution of Na[AuCl 4 ] (0.10 g, 0.28 mmol) in water (2 mL) was added 2,2'-thiodiethanol (0.16 mL). The resulting colorless solution was added to a solution of [1]Cl 2´5 H 2 O (0.20 g, 0.24 mmol) in water (40 mL). The mixture was stirred at RT for 30 min, followed by addition of several drops of a saturated NaCl aqueous solution. The reaction solution was allowed to stand at RT for 5 days, which gave dark green-brown microcrystals. Yield: 0.22 g (82 %); elemental analysis for [Au 2 {Pd[Co(C 6 H 18 N 3 S 3 ) 3 ] 2 } 2 ]Cl 6´1 6 H 2 O: calcd C 12.77, H 4.64, N 7.45; found: C 12.71, H 4.69, N 7.43; 13 C NMR (500 MHz, D 2 O, DSS): d 34.86, 35.55, 40.40 (CH 2 S), 48.44, 56.00, 57.22 (CH 2 N); UV/Vis (H 2 O): s max /10 3 (log e) 18.36 (3.05), 26.91 (4.63), 30.56 (4.76), 33.6 (4.7 sh), 43.6 cm À1 (4.8 m À1 cm À1 sh). [3](NO 3 ) 6´9 H 2 O: To a solution of [1](NO 3 ) 2´1 .5 H 2 O (0.20 g, 0.24 mmol) in water (40 mL), AgNO 3 (0.04 g, 0.24 mmol) was added. The mixture was stirred at RT for 30 min, followed by addition of several drops of a saturated aqueous NaNO 3 . The reaction solution was allowed to stand at RT for 5 days, which gave dark green-brown microcrystals. Yield: 0.20 g (80 %); elemental analysis for [Ag 2 {Pd[Co(C 6 H 18 N 3 S 3 ) 3 ] 2 } 2 ](NO 3 ) 6´9 H 2 O: calcd : C 13.65, H 4.29, N 11.94; found: C 13.44, H 4.28, N 11.71; 13 C NMR (500 MHz, D 2 O, DSS): d 33.03, 35.21, 39.27 (CH 2 S), 50.02, 55.98, 57.25 (CH 2 N); UV/Vis (H 2 O): s max /10 3 (log e) 17.82 (3.00), 27.1 (4.6 sh), 30.56 (4.85), 33.8 (4.7 sh), 43.7 cm À1 (4.8 m À1 cm À1 sh).[1] a) J. M. Lehn, Supermolecular Chemistry: Concepts and Perspectives,

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The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins

Cited by 638 publications

References 56 publications

Analysis of iron–sulfur protein maturation in eukaryotes

Analysis of iron–sulfur protein maturation in eukaryotes

Yeast ABC transporters – A tale of sex, stress, drugs and aging

Proton-Coupled Electron Transfer from Sulfur: A S-H/S-D Kinetic Isotope Effect of ≥31.1

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