The mitochondrial genomes of three species of poison frogs (Anura: <i>Dendrobates</i>)

Lyra, Mariana L.; Sanchez, Eugenia; Kuenzel, Sven; Loetters, Stefan; Haddad, Célio F. B.; Vences, Miguel

doi:10.1080/23802359.2017.1347830

Cited by 2 publications

(2 citation statements)

References 12 publications

(17 reference statements)

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“…In this report we have illustrated how publicly available transcriptome data can be used to clarify the systematics of a taxonomically challenging group of cryptic disease-vector species. This transcriptome-based approach to molecular systematics has, to our knowledge, not been used before in vector studies; it is overall analogous to the assembly of mitochondrial genes from transcriptome data used to study, for example, poison frogs [32], catfish [33], true bugs [34] or ants [35] (see also [36]). We found evidence confirming that R. montenegrensis , a species described in 2012 [7], is genetically indistinguishable from R. robustus II, one of the sibling taxa within R. robustus ( s.l. )…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex

et al. 2019

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Background Rhodnius montenegrensis (Triatominae), a potential vector of Chagas disease, was described after R. robustus -like bugs from southwestern Amazonia. Mitochondrial cytb sequence near-identity with sympatric R. robustus (genotype II) raised doubts about the taxonomic status of R. montenegrensis , but comparative studies have reported fairly clear morphological and genetic differences between R. montenegrensis and laboratory stocks identified as R. robustus . Here, we use a transcriptome-based approach to investigate this apparent paradox. Results We retrieved publicly-available transcriptome sequence-reads from R. montenegrensis and from the R. robustus stocks used as the taxonomic benchmark in comparative studies. We (i) aligned transcriptome sequence-reads to mitochondrial ( cytb ) and nuclear (ITS2, D2- 28S and AmpG ) query sequences (47 overall) from members of the R. prolixus – R. robustus cryptic–species complex and related taxa; (ii) computed breadth- and depth-coverage for the 259 consensus sequences generated by these alignments; and, for each locus, (iii) appraised query sequences and full-breadth-coverage consensus sequences in terms of nucleotide-sequence polymorphism and phylogenetic relations. We found evidence confirming that R. montenegrensis and R. robustus genotype II are genetically indistinguishable and, hence, implying that they are, in all likelihood, the same species. Furthermore, we found compelling genetic evidence that the benchmark ‘ R. robustus ’ stocks used in R. montenegrensis description and in later transcriptome-based comparisons are in fact R. prolixus , although likely mixed to some degree with R. robustus (probably genotype II, a.k.a. R. montenegrensis ). Conclusions We illustrate how public-domain genetic/transcriptomic data can help address challenging issues in disease-vector systematics. In our case-study, taxonomic confusion apparently stemmed from the misinterpretation of sequence-data analyses and misidentification of taxonomic-benchmark stocks. More generally, and together with previous reports of mixed and/or misidentified Rhodnius spp. laboratory colonies, our results call into question the conclusions of many studies (on morphology, genetics, physiology, behavior, bionomics or interactions with microorganisms including trypanosomes) based on non-genotyped ‘ R. prolixus ’ or ‘ ...

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Section: Discussionmentioning

confidence: 99%

Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex

et al. 2019

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“…To confirm the phylogenetic relationships among Bufonidae, 19 sequences of complete or partial mitochondrial genomes were used. The data set was inclusive of the ingroups of the 2 species from this study, 13 other species from Bufonidae (Cao et al, 2006;Dong & Yang, 2016;Igawa et al, 2008;Jacob Machado et al, 2018;Jiang et al, 2017b;Machado, Lyra & Grant, 2016;Wang et al, 2013;Yang et al, 2016;Zhang et al, 2005;Zhang et al, 2013) and the outgroups of 4 species from Mannophryne and Dendrobatidae (Lyra et al, 2017;Zhang et al, 2013). Accession numbers of all mitogenomes are summarized in Table 1.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Characterization of the mitochondrial genomes of two toads, Anaxyrus americanus (Anura: Bufonidae) and Bufotes pewzowi (Anura: Bufonidae), with phylogenetic and selection pressure analyses

Cai

Qin

Zhang

et al. 2020

PeerJ

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Mitogenomes are useful in analyzing phylogenetic relationships and also appear to influence energy metabolism, thermoregulation and osmoregulation. Much evidence has accumulated for positive selection acting on mitochondrial genes associated with environmental adaptation. Hence, the mitogenome is a likely target for environmental selection. The family Bufonidae (true toads) has only nine complete and four partial mitogenomes published compared to the 610 known species of this family. More mitogenomes are needed in order to obtain a clearer understanding of the phylogenetic relationships within Bufonidae that are currently controversial. To date, no mitogenomes have been reported from the genera Anaxyrus and Bufotes. Anaxyrus americanus can live in low temperature environments and Bufotes pewzowi can live in high salinity environments. We sequenced the mitogenomes of these two species to discuss the phylogenetic relationships within Bufonidae and the selection pressures experienced by specimens living in low temperature or saline environments. Like other toads, the circular mitogenomes of both species contained the typical 37 genes. Anaxyrus americanus had the highest A+T content of the complete mitogenome among the Bufonidae. In addition, A. americanus showed a negative AT-skew in the control region, whereas Bufotes pewzowi showed a positive AT-skew. Additionally, both toad species had unique molecular features in common: an ND1 gene that uses TTG as the start codon, an extra unpaired adenine (A) in the anticodon arm of trnS (AGY), and the loss of the DHU loop in trnC. The monophyly of Bufonidae was corroborated by both BI and ML trees. An analysis of selective pressure based on the 13 protein coding genes was conducted using the EasyCodeML program. In the branch model analysis, we found two branches of A. americanus and Bufotes pewzowi that were under negative selection. Additionally, we found two positively selected sites (at positions 115 and 119, BEB value > 0.90) in the ND6 protein in the site model analysis. The residue D (119) was located only in A. americanus and may be related to adaptive evolution in low temperature environments. However, there was no evidence of a positively selected site in Bufotes pewzowi in this study.

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The mitochondrial genomes of three species of poison frogs (Anura: Dendrobates)

Cited by 2 publications

References 12 publications

Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex

Transcriptome-based molecular systematics: Rhodnius montenegrensis (Triatominae) and its position within the Rhodnius prolixus–Rhodnius robustus cryptic–species complex

Characterization of the mitochondrial genomes of two toads, Anaxyrus americanus (Anura: Bufonidae) and Bufotes pewzowi (Anura: Bufonidae), with phylogenetic and selection pressure analyses

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