The mitochondrial genome sequence of Enterobius vermicularis (Nematoda: Oxyurida) — An idiosyncratic gene order and phylogenetic information for chromadorean nematodes

Kang, Soo Yong; Sultana, Tahera; Eom, Keeseon S.; Park, Yung Chul; Soonthornpong, Nathan; Nadler, Steven A.; Park, Joong Ki

doi:10.1016/j.gene.2008.09.011

Cited by 56 publications

(69 citation statements)

References 47 publications

(82 reference statements)

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“…However, findings from the present study along with results from several recent investigations (Kim et al 2006;Jex et al 2009;Kang et al 2009) pertaining to nematode mt genome analysis indicate that Ascaridida have a closer relationship with Rhabditida instead. Furthermore, gene arrangement patterns were more similar between Ascaridida and Rhabditida rather than Spirurida.…”

Section: Phylogenetic Analysiscontrasting

confidence: 51%

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The complete mitochondrial genome of the rodent intra-arterial nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis

Zhang

et al. 2012

Parasitol Res

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The two rodent intra-arterial nematodes, Angiostrongylus cantonensis and Angiostrongylus costaricensis, can cause human ill-health. The present study aimed to characterize and compare the mitochondrial (mt) genomes of these two species, and clarify their phylogenetic relationship and the position in the phylum Nematoda. The complete mt genomes of A. cantonensis and A. costaricensis are 13,497 and 13,585 bp in length, respectively. Hence, they are the smallest in the class of Chromadorea characterized thus far. Like many nematode species in the class of Chromadorea, they encode 12 proteins, 22 transfer RNAs, and two ribosomal RNAs. All genes are located on the same strand. Nucleotide identity of the two mt genomes is 81.6%, ranging from 77.7% to 87.1% in individual gene pairs. Our mt genome-wide analysis identified three major gene arrangement patterns (II-1, II-2, and II-3) from 48 nematode mt genomes. Both patterns II-1 and II-2 are distinct from pattern II-3, which covers the Spirurida, supporting a closer relationship between Ascaridida and Strongylida rather than Spirurida. Thymine (T) was highly concentrated on coding strands in Chromadorea, but balanced between the two strands in Enoplea, probably due to the gene arrangement pattern. Interestingly, the gene arrangement pattern of mt genomes and phylogenetic analysis based on concatenated amino acids indicated a closer relationship between the order Ascaridida and Rhabditida rather than Spirurida as indicated in previous studies. These discrepancies call for new research, reassessing the position of the order of Ascaridida in the phylogenetic tree. Once consolidated, the findings are important for population genetic studies and target identification.

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Section: Phylogenetic Analysiscontrasting

confidence: 51%

“…2). Overall, UUU (Phe), UUG (Leu), UUA (Leu), GUU (Val), UAU (Tyr), and AUU (Ile) are dominant codons, which is similar to other nematode species (Lavrov and Brown 2001;Hu et al 2002Hu et al , 2003aMontiel et al 2006;Kang et al 2009). However, the proportion of these codons showed a marked variation in different genes.…”

Section: Resultsmentioning

confidence: 54%

The complete mitochondrial genome of the rodent intra-arterial nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis

Zhang

et al. 2012

Parasitol Res

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“…Molecular characteristics of E. vermicularis isolates described in this work are the starting point for defi ning the genetic diversity of human pinworm occurring in the child population in Poland. All of the isolates studied were different in terms of the sequences within the cytochrome c oxidase subunit 1 (cox1) gene; two of them, however, were identical with those earlier identifi ed in the E. ver- (Ferrero et al, 2013) and Japan (Kang et al,. 2009).…”

Section: Discussionmentioning

confidence: 66%

“…2006;Piperaki et al, 2011;Zelck et al, 2011;Ferrero et al, 2013). Complete data on the DNA sequence are only available in relation to the mitochondrial genome of E. vermicularis (Kang et al, 2009). The analysis of the mitochondrial cox1 gene sequences in pinworm isolates from humans and captive chimpanzees (Pan troglodytes) has shown the presence of three different genetic types, designated as type A, B and C (Nakano et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

2017

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SummaryEnterobiasis is a human intestinal parasitic disease caused by human pinworm, Enterobius vermicularis. Despite being the most prevalent nematode infection in Europe and North America, predominantly among in school aged children, the data concerning infection rate and knowledge of genetic variability of pinworms are incomplete. The aim of the study was the estimation of prevalence and molecular typing of Enterobius vermicularis among healthy children in north-eastern Poland. In 2013 -2015, 296 individuals (aged 2 -18 years) from 12 kindergartens, schools and orphanages were examined by the adhesive cellophane tape method. Data on socio-demographic status were collected using a questionnaire. Molecular analysis was performed using the DNA of adult female pinworms and primers targeting the region of cytochrome oxidase I gene. The overall prevalence of enterobiasis was 10.1 %. Enterobius vermicularis infection rates were 3.9 % in children living in families and 32.8 % among the orphans (OR=0.08; 95 % CI: 0.04 -0.19; p<0.001). There were no associations between distribution of enterobiasis and gender, pets possession and the season of examination. In 43.3 % of the infected children enterobiasis was asymptomatic. Based on a molecular marker three different haplotypes of pinworm were identifi ed. All sequences clustered within type B, together with human E. vermicularis isolates from Denmark, Germany, Greece, and Japan. This paper provides complementary data on the occurrence and intraspecifi c variability of E. vermicularis in human population in Europe.

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“…Molecular tools might help to understand transmission routes and distinguish persistent from repeated infections. However, sequence information on E. vermicularis is limited (6,7).…”

mentioning

confidence: 99%

Molecular Phylogenetic Analysis of Enterobius vermicularis and Development of an 18S Ribosomal DNA-Targeted Diagnostic PCR

Zelck

Bialek²,

Weiß

2011

J Clin Microbiol

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We genetically characterized pinworms obtained from 37 children from different regions of Germany and established new species-specific molecular diagnostic tools. No ribosomal DNA diversity was found; the phylogenetic position of Enterobius vermicularis within the Oxyurida order and its close relationship to the Ascaridida and Spirurida orders was confirmed.Pinworms are the most common intestinal parasites in developed countries in temperate climates (1). Humans are the only natural host of Enterobius vermicularis, and children are the most often affected by this disease, which is spread from the anus to mouth. Although it is regarded as a harmless infection, severe disease like colitis, perianal abscess, ectopic infections in females, and appendicitis can occur (1,3,12). Whereas eradication is achieved by anthelmintics in most cases (12), recurrent or persistent oxyuriasis lasting for years despite several treatment courses is seen in some patients. Molecular tools might help to understand transmission routes and distinguish persistent from repeated infections. However, sequence information on E. vermicularis is limited (6, 7).Pinworms were identified by examining feces from children with a magnifier, washed in tap water, and stored at Ϫ20°C. Then, pinworms from 37 children residing in different geographic regions of Germany were thawed and mechanically homogenized to extract DNA (Qiamp DNA minikit; Qiagen, Hilden, Germany). Enterobius vermicularis ribosomal DNA (rDNA) was amplified and sequenced as partially overlapping fragments using universal primers (AB28/TW81 and NEMF1/ S3), degenerated nematode primers, and species-specific primers (Table 1). Amplification reaction mixtures (50 l) consisted of 100 nM (each) primer, 50 M (each) deoxynucleoside triphosphates (dNTPs), 2.5 mM MgCl 2 , 0.5 units polymerase, and 10 l template. PCR amplification was performed as follows: (i) denaturation at 95°C for 5 min; (ii) 40 cycles, with 1 cycle consisting of 60 s at 94°C, 60 s at 50 to 60°C, and 2 min at 72°C for 2 min, and (iii) a final extension step at 72°C for 10 min. Amplification of 18S rDNA fragments for diagnostic purposes using Enterobius-specific primers Ev18S.F1 and Ev18S.R1 was performed at 55°C under otherwise identical conditions. Products were detected on ethidium bromidestained agarose gels. PCR products were sequenced either directly or after gel extraction (QIAquick gel extraction kit; Qiagen, Hilden, Germany) and cloning (TOPO-TA; Invitrogen, Karlsruhe, Germany) using a BigDye terminator cycle sequencing kit and an ABI Prism 310 genetic analyzer (Applied Biosystems, Warrington, United Kingdom). Site polymorphisms were scored when alternative nucleotide peaks present were equal in height or when a minor peak significantly exceeded the background level and comprised Ն50% of the major peak. We amplified and sequenced the DNA of the small ribosomal subunit (18S rDNA, 1,716 bp), first and second internal transcribed spacer regions (ITS1 and ITS2, 1,073 bp) including the 5.8S rDNA (159 bp), and a 78-bp fragm...

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The mitochondrial genome sequence of Enterobius vermicularis (Nematoda: Oxyurida) — An idiosyncratic gene order and phylogenetic information for chromadorean nematodes

Cited by 56 publications

References 47 publications

The complete mitochondrial genome of the rodent intra-arterial nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis

The complete mitochondrial genome of the rodent intra-arterial nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis

Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

Molecular Phylogenetic Analysis of Enterobius vermicularis and Development of an 18S Ribosomal DNA-Targeted Diagnostic PCR

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