The mitochondrial genome of yeast Hansenula wingei encodes NADH dehydrogenase subunit genes ND4L and ND5

Okamoto, Kozi; Sekito, Takayuki; Yoshida, Kazuo

doi:10.1007/bf00280478

Cited by 9 publications

(5 citation statements)

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“…They are the same set of proteins as those of S. cerevisiae (see Table 1): the ATPase subunit genes ATP6 , 8 , 9 , the KlCYTB coding for apocytochrome b , the cytochrome oxidase subunit genes KlCOX1 , 2 and 3 , and a ribosomal protein KlVAR1 . The NADH dehydrogenase subunit genes that are usually present in aerobic yeasts like Hansenula wingei [13], Yarrowia lipolytica [14] or Candida parapsilosis [15] are absent from K. lactis mtDNA. However, several short open reading frames in the intergenic regions show a limited resemblance to parts of NADH dehydrogenase subunit genes (17968–17843 to ND2 , 20341–20625, 9219–9022 and 4179–4036 to ND5 , 36380–36240 to ND6 , and 28714–28836 to ND1 ).…”

Section: Resultsmentioning

confidence: 99%

Complete nucleotide sequence of the mitochondrial DNA from

Zivanovic

Wincker

Vacherie

et al. 2005

FEMS Yeast Research

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The total nucleotide sequence of the mitochondrial genome of the yeast Kluyveromyces lactis was determined. The DNA is a circular molecule of 40,291 base pairs, with 26.1% GC. It contains a set of protein- and RNA-coding genes equivalent to those of the Saccharomyces cerevisiae mitochondrial genome. The genome size is about one half of that of S. cerevisiae mitochondrial DNA. The difference in size is due essentially to a reduced proportion of intergenic and intronic sequences. The coding sequences occupy about one third of the genome, the rest being composed of AT-rich sequences and numerous short GC-rich clusters that are dispersed mostly in the non-coding regions and a few within coding sequences. The presence of these GC clusters is a characteristic feature common to K. lactis and S. cerevisiae mitochondrial DNA, although their sequence patterns are different. The absence of the NADH dehydrogenase subunit genes distinguishes this yeast and S. cerevisiae from the typically aerobic species. The genetic code appears to be that of the standard fungal mitochondrial genomes, with UGA as a tryptophan codon. There are only 22 transfer RNA genes, those corresponding to CUN and CGN codons being missing. CUN codons are absent in the protein-coding sequences. There are five CGN codons within the open reading frames, but they are located exclusively in the introns, rendering them untranslatable. Introns are found only the genes in KlCOX1 and LrRNA. The transcription promoter motif known in S. cerevisiae and several other yeast species is also present. All genes are transcribed from the same strand, except those on a single 7-kilobase pairs segment (EMBL Accession No. AY654900).

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Section: Resultsmentioning

confidence: 99%

Complete nucleotide sequence of the mitochondrial DNA from

Zivanovic

Wincker

Vacherie

et al. 2005

FEMS Yeast Research

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“…The mitochondrial genome of Y. lipolytica strain W29 consists of a circular molecule with a size of 47.9 kb. This is intermediate between the compact 20 kb and 27.7 kb mt genomes of T. glabrata (Clark‐Walker, 1992) and H. wingei (Okamoto et al ., 1994; Sekito et al ., 1995) and the large 100.3 kb mt genome of P. anserina (Cummings et al ., 1990). All genes are encoded on the same strand, as commonly observed among ascomycetous fungi, with the notable exception of S. cerevisiae , where a single tRNA gene ( thr1 ) resides on the opposite strand (Foury, 1998).…”

Section: Resultsmentioning

confidence: 99%

“…Other fungi, such as Neurospora crassa and Aspergillus nidulans , have both a mitochondrial and a nuclear gene for ATP9, with the nuclear gene being the active one (van den Boogaart et al ., 1982; Brown et al ., 1985). Some fungal mt genomes also contain additional genes, encoding accessory ribosomal proteins such as VAR1 in S. cerevisiae (Hudspeth et al ., 1982), Torulopsis glabrata (Ainley et al ., 1985) and H. wingei (Okamoto et al ., 1994; Sekito et al ., 1995) or SP5 in N. crassa (Collins, 1993), or the RNA component of RNase P in S. cerevisiae (Foury, 1998). The mould mitochondrial genetic code appears to be well conserved, with the UGA codon being read as tryptophan as the only exception from the universal genetic code.…”

Section: Introductionmentioning

confidence: 99%

The complete mitochondrial genome of Yarrowia lipolytica

et al. 2001

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We here report the complete nucleotide sequence of the 47.9 kb mitochondrial (mt) genome from the obligate aerobic yeast Yarrowia lipolytica. It encodes, all on the same strand, seven subunits of NADH: ubiquinone oxidoreductase (ND1-6, ND4L), apocytochrome b (COB), three subunits of cytochrome oxidase (COX1, 2, 3), three subunits of ATP synthetase (ATP6, 8 and 9), small and large ribosomal RNAs and an incomplete set of tRNAs. The Y. lipolytica mt genome is very similar to the Hansenula wingei mt genome, as judged from blocks of conserved gene order and from sequence homology. The extra DNA in the Y. lipolytica mt genome consists of 17 group 1 introns and stretches of A+Trich sequence, interspersed with potentially transposable GC clusters. The usual mould mt genetic code is used. Interestingly, there is no tRNA able to read CGN (arginine) codons. CGN codons could not be found in exonic open reading frames, whereas they do occur in intronic open reading frames. However, several of the intronic open reading frames have accumulated mutations and must be regarded as pseudogenes. We propose that this may have been triggered by the presence of untranslatable CGN codons. This sequence is available under EMBL Accession No. AJ307410.

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“…CYTB (or COB), encoding the apocytochrome b; ATP6, ATP8 and ATP9, three subunits of the ATP synthetase; COX1, COX2 and COX3, three subunits of the cytochrome oxidase; and VAR1, a ribosomal protein (Foury et al, 1998;Koszul et al, 2003;Talla et al, 2005;Zivanovic et al, 2005). These mitochondrial genomes do not encode subunits of the NADH : ubiquinone oxidoreductase (complex I), in contrast to all other Hemiascomycetes, such as Candida parapsilosis (Nosek & Fukuhara, 1994), Hansenula wingei (Okamoto et al, 1994) or Yarrowia lypolytica (Kerscher et al, 2001). In addition, yeast mitochondrial genomes encode the large (LSU) and small (SSU) rRNAs, the RNA component of the RNAse P and a set of tRNA genes.…”

Section: Introductionmentioning

confidence: 99%

Complete mitochondrial genome sequences ofâ threeNakaseomycesspecies reveal invasion by palindromic GC clusters and considerable size expansion

Bouchier

Créno

et al. 2009

FEMS Yeast Research

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Here we report the sequence of three mitochondrial genomes from yeasts of the Nakaseomyces clade that includes the pathogenic yeast Candida glabrata, namely, that of Kluyveromyces delphensis, Candida castellii and Kluyveromyces bacillisporus. The gene content is equivalent to that of C. glabrata, but reveals the existence of new group I introns in COX1 and CYTB and new potential intronic endonucleases. Gene order is highly rearranged in these genomes, which contain numerous palindromic GC clusters. The more GC nucleotides these elements contain, the longer and more AT-rich are the intergenes containing them, leading to a direct relationship between the number of Gs and Cs within the elements and the size of the genomes. Thus, there is a fivefold difference in size between the smallest and the largest mitochondrial genome, with the largest being the most AT-rich overall. Sequences are available under EMBL accession numbers FM995164, FM995165, and FM995166.

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The mitochondrial genome of yeast Hansenula wingei encodes NADH dehydrogenase subunit genes ND4L and ND5

Cited by 9 publications

References 10 publications

Complete nucleotide sequence of the mitochondrial DNA from

Complete nucleotide sequence of the mitochondrial DNA from

The complete mitochondrial genome of Yarrowia lipolytica

Complete mitochondrial genome sequences ofâ threeNakaseomycesspecies reveal invasion by palindromic GC clusters and considerable size expansion

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The mitochondrial genome of yeast Hansenula wingei encodes NADH dehydrogenase subunit genes ND4L and ND5

Cited by 9 publications

References 10 publications

Complete nucleotide sequence of the mitochondrial DNA from

Complete nucleotide sequence of the mitochondrial DNA from

The complete mitochondrial genome of Yarrowia lipolytica

Complete mitochondrial genome sequences ofâ threeNakaseomycesspecies reveal invasion by palindromic GC clusters and considerable size expansion

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Product

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Complete mitochondrial genome sequences ofâ threeNakaseomycesspecies reveal invasion by palindromic GC clusters and considerable size expansion