The mitochondrial elongation factor LeEF-Tsmt is regulated during tomato fruit ripening and upon wounding and ethylene treatment

Girardi, C. L.; Bermudez, Kalina; Bernadac, Anne; Chavez, Ana Cristina Delgado; Zouine, Mohamed; Jaimes-Miranda, Fabiola; Bouzayen, Mondher; Pech, Jean‐Claude; Latché, Alain

doi:10.1016/j.postharvbio.2006.06.002

Cited by 6 publications

(4 citation statements)

References 21 publications

(27 reference statements)

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“…Anthocyanins are synthesized through the flavonoid biosynthetic pathway [46] . Therefore, expression levels of tomato genes for Phe-ammonia lyase ( PAL ), the flavonoid pathway genes chalcone synthase ( CHS ), dihydroflavonol reductase ( DFR ), and anthocyanidin synthase ( ANS ) were determined and expressed relative to the LeActin gene, a tomato gene that encodes an actin protein [39] . Consistent with the reduced anthocyanin accumulation in 35S::CPC transgenic tomato ( Figure 4B ), PAL , CHS , DFR and ANS expression levels were significantly lower in 35S::CPC transgenic tomato compared with the control plants ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

“…The relative expression of each transcript was calculated by the ΔΔCT method [38] . The expression levels of CPC , GL3 , PAL , CHS , DFR and ANS were estimated after being normalized to the endogenous control gene LeActin (TC116322) [39] . The primers were: CPC-F and CPC-R for CPC ; GL3-F and GL3-R for GL3 ; PAL-F and PAL-R for PAL ; CHS-F and CHS-R for CHS ; DFR-F and DFR-R for DFR ; ANS-F and ANS-R for ANS ; LeActin-F and LeActin-R for LeActin [39] – [41] .…”

Section: Methodsmentioning

confidence: 99%

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Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) Control Tomato (Solanum lycopersicum) Anthocyanin Biosynthesis

2014

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In Arabidopsis thaliana the MYB transcription factor CAPRICE (CPC) and the bHLH transcription factor GLABRA3 (GL3) are central regulators of root-hair differentiation and trichome initiation. By transforming the orthologous tomato genes SlTRY (CPC) and SlGL3 (GL3) into Arabidopsis, we demonstrated that these genes influence epidermal cell differentiation in Arabidopsis, suggesting that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation. CPC and GL3 are also known to be involved in anthocyanin biosynthesis. After transformation into tomato, 35S::CPC inhibited anthocyanin accumulation, whereas GL3::GL3 enhanced anthocyanin accumulation. Real-time reverse transcription PCR analyses showed that the expression of anthocyanin biosynthetic genes including Phe-ammonia lyase (PAL), the flavonoid pathway genes chalcone synthase (CHS), dihydroflavonol reductase (DFR), and anthocyanidin synthase (ANS) were repressed in 35S::CPC tomato. In contrast, the expression levels of PAL, CHS, DFR, and ANS were significantly higher in GL3::GL3 tomato compared with control plants. These results suggest that CPC and GL3 also influence anthocyanin pigment synthesis in tomato.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) Control Tomato (Solanum lycopersicum) Anthocyanin Biosynthesis

2014

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“…The expression levels of GL2 and GFP were estimated after being normalized to the endogenous control gene ACT2 (AB026654). The primers were: RTSlTRY-F and RTSlTRY-R for SlTRY ; RTSlGL3-F and RTSlGL3-R for SlGL3 ; LeActin-F and LeActin-R for LeActin [47]; GL2-F and GL2-R for GL2 [48]; GFP-F and GFP-R for GFP ; and Act2-F and Act2-R for ACT2 [49].…”

Section: Methodsmentioning

confidence: 99%

Control of Plant Trichome and Root-Hair Development by a Tomato (Solanum lycopersicum) R3 MYB Transcription Factor

et al. 2013

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In Arabidopsis thaliana the CPC-like MYB transcription factors [CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER OF TRY AND CPC 1, 2, 3/CPC-LIKE MYB 3 (ETC1, ETC2, ETC3/CPL3), TRICHOMELESS 1, 2/CPC-LIKE MYB 4 (TCL1, TCL2/CPL4)] and the bHLH transcription factors [GLABRA3 (GL3) and ENHANCER OF GLABRA 3 (EGL3)] are central regulators of trichome and root-hair development. We identified TRY and GL3 homologous genes from the tomato genome and named them SlTRY and SlGL3, respectively. Phylogenic analyses revealed a close relationship between the tomato and Arabidopsis genes. Real-time reverse transcription PCR analyses showed that SlTRY and SlGL3 were predominantly expressed in aerial parts of developing tomato. After transformation into Arabidopsis, CPC::SlTRY inhibited trichome formation and enhanced root-hair differentiation by strongly repressing GL2 expression. On the other hand, GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation. These results suggest that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation, and that a CPC-like R3 MYB may be a key common regulator of plant trichome and root-hair development.

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“…OsUBQ and LeAction were used as the endogenous controls to normalize the expression levels of OsGL2 or SlGL2, respectively. The primers were: OsGL2-qF and OsGL2-qR for OsGL2 (Zheng et al 2016), UBQ5-qPCRF and UBQ5-qPCRR for OsUBQ (Guo et al 2015), SlGL2RT-F and SlGL2RT-R for SlGL2 (Lashbrooke et al 2015), and LeAction-F and LeAction-R for LeAction (Girardi et al 2006).…”

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confidence: 99%

Effect of the CLE14 polypeptide on <i>GLABRA2</i> homolog gene expression in rice and tomato roots

Hayashi

Rongkavilit

Tetsumura

et al. 2019

Plant Biotechnology

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The CLAVATA3/ESR (CLE) plant polypeptides act as peptide hormones in various physiological and developmental aspects in a diverse array of land plants. One of the CLE family of genes, CLE14, is reported to induce root hair formation in Arabidopsis thaliana roots. Previously, we demonstrated that the application of synthetic CLE14 polypeptide treatment induced excess root hairs, and reduced the expression level of the non-hair cell fate determinant gene, GLABRA2 (GL2) in Arabidopsis roots. In this study, we investigated the function of synthetic CLE14 polypeptide in rice (Oryza sativa) and tomato (Solanum lycopersicum) roots. We measured the expression levels of the OsGL2 and SlGL2 genes, i.e., homologs of the Arabidopsis GL2 gene, in rice and tomato seedlings, respectively. Although CLE14 polypeptide treatment induced excess root hair formation in rice roots, substantial root hair induction was not observed in tomato roots. However, the CLE14 polypeptide treatment significantly inhibited the expression of the GL2 homolog genes of rice (OsGL2) and tomato (SlGL2). Our findings thus indicated that CLE14 can inhibit the GL2 gene expression in both rice and tomato plants, similar to the effect seen in Arabidopsis.

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The mitochondrial elongation factor LeEF-Tsmt is regulated during tomato fruit ripening and upon wounding and ethylene treatment

Cited by 6 publications

References 21 publications

Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) Control Tomato (Solanum lycopersicum) Anthocyanin Biosynthesis

Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) Control Tomato (Solanum lycopersicum) Anthocyanin Biosynthesis

Control of Plant Trichome and Root-Hair Development by a Tomato (Solanum lycopersicum) R3 MYB Transcription Factor

Effect of the CLE14 polypeptide on <i>GLABRA2</i> homolog gene expression in rice and tomato roots

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