The miR408 expression in scutellum derived somatic embryos of Oryza sativa L. ssp. indica varieties: media and regenerating embryos

Solanki, Manish; Sinha, Anshika; Shukla, Lata I.

doi:10.1007/s11240-019-01602-w

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…Increasing numbers of miRNAs have been annotated in various plant species (Solanki et al., 2019; Zhang et al., 2009; Zhao et al., 2010); however, few have been reported for barley (Kantar et al., 2010; Lv et al., 2012; Qiu et al., 2019). MicroRNAs play important roles in the process of plant resistance to disease (Chinen, 2012).…”

Section: Discussionmentioning

confidence: 99%

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

et al. 2021

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Tibetan hulless barley (Hordeum vulgare L. variety nudum Hook. f.) is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Here, we compared the miRNA profiles before and after BLS in a BLS-sensitive ('Z1141') and tolerant ('Kunlun14') Tibetan hulless barley for the first time. A total of 36 conserved and 56 novel miRNAs were identified, of which 24 differentially expressed miRNAs were found in Z1141 and Kunlun14, and an additional 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among these genes, 131 were differentially expressed in normal and infected leaves of Kunlun14 and Z1141.Gene ontology (GO), pathway, and Blast analyses indicated 10 candidate target genes including seven functional genes and three transcription factors were involved in barley disease resistance. These functional genes were two members of the CYP450 genes, a RGA gene, a LIN gene, a SAM gene, a PSD gene, and a NDB gene; the three transcription factor genes were all from the WRKY family. These 10 candidate target genes were regulated by eight miRNAs (hvu-miR168-3p, hvu-miR171-5p, hvu-miR159b, hvu-miR156a, hvu-novel-91, hvu-novel-46, hvu-novel-52, and hvu-novel-11). According to quantitative real-time polymerase chain reaction (PCR) results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Thus, these miRNAs and their target genes can be used to improve BLS resistance in Tibetan hulless barley though breeding programs or genetic engineering.

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Section: Discussionmentioning

confidence: 99%

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

et al. 2021

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“…Increasing numbers of miRNAs are being annotated in various plant species [41,42]; however, few have been reported for barley [43,44]. miRNAs played important roles in the process of plant resistance to disease [18].…”

Section: Discussionmentioning

confidence: 99%

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

Yao¹,

Wang²,

Yao³

et al. 2020

Preprint

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Background Tibetan hulless barley is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Results Here, we compared the miRNA profiles before and after BLS in two Tibetan hulless barley genotypes: Z1141, a BLS-sensitive wild variety, and Kunlun14, a BLS-tolerant hybrid variety. A total of 36 conserved and 56 novel miRNAs were identified. Of these, 10 conserved and 10 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Kunlun14, respectively, while 3 conserved and 5 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Z1141, respectively. A total of 24 miRNAs were found in Z1141 and Kunlun14, and a further 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among the 546 candidate genes, 131 had significant differences in expression between the normal and infected leaves of Kunlun14 and Z1141. Gene ontology, pathway, and Blast analyses indicated 10 candidate target genes that were involved in the barley disease resistance. These 10 candidate target genes may be regulated by 7 miRNAs. According to quantitative real-time PCR results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Conclusions The miRNAs and their target genes expressed in Tibetan hulless barley were identified and found to be associated with BLS resistance. Thus, these miRNAs and their target genes may be exploited via breeding programs or genetic engineering to improve BLS resistance in Tibetan hulless barley.

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Investigations of scutellum derived calli, cues from size, effective ionic strength of synthetic media and improved regeneration capacity for indica rice

Solanki

Sinha

Shukla

2020

Plant Cell Tiss Organ Cult

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The miR408 expression in scutellum derived somatic embryos of Oryza sativa L. ssp. indica varieties: media and regenerating embryos

Cited by 4 publications

References 38 publications

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

Investigations of scutellum derived calli, cues from size, effective ionic strength of synthetic media and improved regeneration capacity for indica rice

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