The miR172c-NNC1 module modulates root plastic development in response to salt in soybean

Abstract

BackgroundPlant roots are highly plastic to high salinity. However, the molecular mechanism by which root developmental plasticity is regulated remains largely unknown. Previously we reported that miR172c-NNC1 module plays a key role in soybean-rhizobial symbiosis. The fact that the miR172c promoter contains several stress-related cis elements indicates that miR172c may have a role in root response to abiotic stress.ResultsHere we showed that miR172c is greatly induced by salt stress in soybean. Overexpression of miR172c and knockdown of miR172c activity resulted in substantially increased and reduced root sensitivity to salt stress, respectively. Furthermore, we show that the target gene NNC1 (Nodule Number Control 1) of miR172c was downregulated by salt stress. The transgenic roots overexpressing or knocking down NNC1 expression also exhibited the altered root sensitivity to salt stress.ConclusionThe study reveals the crucial role of miR172c-NNC1 module in root stress tolerance to salt stress in soybean.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1161-9) contains supplementary material, which is available to authorized users.

Integration of Transcriptomes, Small RNAs, and Degradome Sequencing to Identify Putative miRNAs and Their Targets Related to Eu-Rubber Biosynthesis in Eucommia ulmoides

“…In this study, a total of 31 DEMs were presumed to be involved in Eu-rubber biosynthesis (Figure 3), including one MIR172 and three MIR398 family members and 27 novel miRNAs. The functions of miR172 in flowering timing [48], nodulation in legumes [49,50,51,52], developmental phase transition [53], and response to salt stress [54] are well known. Additionally, previous studies had shown that the expression levels of miR172 were significantly negatively correlated with the content of latex and Eu-rubber [10,12].…”

High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.)

“…miRNAs are indispensable for plants to respond to salt stress and play an important regulatory role by regulating the expression of their target genes. For example, miR396 increases Arabidopsis salt tolerance through regulating its target gene GRF [13]; miR398 protects cell membrane structure against salt stress by targeting CSD1 and CSD2 [14]; The miR172c-NNC1 module modulates root plastic development in response to salt in soybean [15]; and the target gene of miR169c can control the stomatal opening and closure in maize leaves, thereby reducing water loss and resisting salt stress [16]. The decrease in miR414/miR408/miR164e leads to upregulated helicase (OsABP, OsDSHCT and OsDBH) expression, thereby regulating gene recombination replication and repair to resist saline environment [17].…”

Genome-scale characterization of the vacuole nitrate transporter Chloride Channel (CLC) genes and their transcriptional responses to diverse nutrient stresses in allotetraploid rapeseed

“…Moreover, excluding the common CREs, such as TATA-box, CAAT-box, and light-responsive elements (e.g., GATA-box and GT1CONSENSUS) [60], some phytohormone responsive elements were identified. These included ARR1AT (NGATT), cytokinin-responsive elements, and abscisic acid-responsive elements, such as ABRELATERD1, ACGTATERD1, MYB, and MYC [61]. The copper-responsive element (CURE) was the only mineral nutrient-responsive element detected in the BnaCLC genes.…”