The miR-143-<i>adducin3</i> pathway is essential for cardiac chamber morphogenesis

Deacon, Dekker C.; Nevis, Kathleen R.; Cashman, Timothy J.; Zhou, Yong; Zhao, Long; Washko, Daniel; Guner-Ataman, Burcu; Burns, C. Geoffrey; Burns, Caroline E.

doi:10.1242/dev.050526

Cited by 89 publications

(69 citation statements)

References 53 publications

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“…The ejection fraction (EF) of the ventricle was 2272% at 48 hpf, comparable to a previous report (Deacon et al, 2010) and then 21.5 71.6%, 21.7 71.5%, 2372%, respectively at 72, 96 and 120 hpf ( Fig. 4) .…”

Section: Functional Changes Of the Ventricle During Developmentsupporting

confidence: 90%

Temporal cohesion of the structural, functional and molecular characteristics of the developing zebrafish heart

et al. 2015

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Heart formation is a complex, dynamic and highly coordinated process of molecular, morphogenetic and functional factors with each interacting and contributing to formation of the mature organ. Cardiac abnormalities in early life can be lethal in mammals but not in the zebrafish embryo which has been widely used to study the developing heart. While early cardiac development in the zebrafish has been well characterized, functional changes during development and how these relate to architectural, cellular and molecular aspects of development have not been well described previously. To address this we have carefully characterised cardiac structure, function, cardiomyocyte proliferation and cardiac-specific gene expression between 48 and 120 hpf in the zebrafish. We show that the zebrafish heart increases in volume and changes shape significantly between 48 and 72 hpf accompanied by a 40% increase in cardiomyocyte number. Between 96 and 120 hpf, while external heart expansion slows, there is rapid formation of a mature and extensive trabecular network within the ventricle chamber. While ejection fraction does not change during the course of development other determinants of contractile function increase significantly particularly between 72 and 96 hpf leading to an increase in cardinal vein blood flow. This study has revealed a number of novel aspects of cardiac developmental dynamics with striking temporal orchestration of structure and function within the first few days of development. These changes are associated with changes in expression of developmental and maturational genes. This study provides important insights into the complex temporal relationship between structure and function of the developing zebrafish heart.

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Section: Functional Changes Of the Ventricle During Developmentsupporting

confidence: 90%

Temporal cohesion of the structural, functional and molecular characteristics of the developing zebrafish heart

et al. 2015

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“…miR-143 is one of the most enriched miRs during embryonic development, with an essential role for myocardial proliferation, cardiac function and cardiogenesis. 28 Besides, miR-143 also showed reversed expression in some carcinomas and regenerative from Dr. Xu Meifeng (University of Cincinnati) and Dr. Ge Ruowen (National University of Singapore). The vectors were individually propagated in HEK-293 cells cultured in DMEM supplemented with 10% fetal bovine serum (FBS).…”

Section: Discussionmentioning

confidence: 98%

MicroRNA-143 is a critical regulator of cell cycle activity in stem cells with co-overexpression of Akt and angiopoietin-1 via transcriptional regulation of Erk5/cyclin D1 signaling

Lai¹,

Ashraf²,

Jiang³

et al. 2012

Cell Cycle

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“…For instance, RA that is synthesized by raldh2 and diffused out from the endocardium could have a non-cell autonomous effect to the myocardium. Recently, it has been shown that miR-143 regulates adducin3 (add3), which encodes an F-actin capping protein (Deacon et al, 2010). This pathway also contributes to the correct formation of the ventricular architectures, suggesting that multiple cascades regulate chamber formation of the heart through the heartbeat-dependent expression of miR-143.…”

Section: Discussionmentioning

confidence: 99%

Heartbeat regulates cardiogenesis by suppressing retinoic acid signaling via expression of miR-143

Miyasaka

Kida

Banjo

et al. 2011

Mechanisms of Development

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Cardiogenesis proceeds with concomitant changes in hemodynamics to accommodate the circulatory demands of developing organs and tissues. In adults, circulatory adaptation is critical for the homeostatic regulation of blood circulation. In these hemodynamics-dependent processes of morphogenesis and adaptation, a mechanotransduction pathway, which converts mechanical stimuli into biological outputs, plays an essential role, although its molecular nature is largely unknown. Here, we report that expression of zebrafish miR-143 is dependent on heartbeat. Knocking-down miR-143 results in de-repression of retinoic acid signaling, and produces abnormalities in the outflow tracts and ventricles. Our data uncover a novel epigenetic link between heartbeat and cardiac development, with miR-143 as an essential component of the mechanotransduction cascade.

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The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis

Cited by 89 publications

References 53 publications

Temporal cohesion of the structural, functional and molecular characteristics of the developing zebrafish heart

Temporal cohesion of the structural, functional and molecular characteristics of the developing zebrafish heart

MicroRNA-143 is a critical regulator of cell cycle activity in stem cells with co-overexpression of Akt and angiopoietin-1 via transcriptional regulation of Erk5/cyclin D1 signaling

Heartbeat regulates cardiogenesis by suppressing retinoic acid signaling via expression of miR-143

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