The minor histocompatibility antigen HA-3 arises from differential proteasome–mediated cleavage of the lymphoid blast crisis (Lbc) oncoprotein

Minor histocompatibility antigen-specific T cells with multiple distinct specificities can be isolated by direct cloning of IFNγ-secreting T cells from patients with relapsed leukemia responding to donor lymphocyte infusion

“…A limited number of human mHags has been characterized, including HA-1, HA-2, HA-3, HA-8, male-specific HY antigens and the mHags ACC-1 and ACC-2 encoded by distinct polymorphisms within the BCL2A1 gene. [5][6][7][8][9][10][11][12][13][14] The large numbers of nucleotide polymorphisms in the human genome may indicate a potentially large diversity of mHags. However, mouse transplantation models have indicated that immune responses mainly occur against a limited number of mHags.…”

Recognition of allo-peptide is governed by novel anchor imposition and limited variations in TCR contact residues

“…Antigenic peptides from mH-Ags have served as useful models to understand antigen processing and presentation (Spierings et al, 2003;Engelhard et al, 2002;Malarkannan et al, 1999), immunodominance (van Els et al, 1992;Wolpert et al, 1998;Choi et al, 2002), graft rejections (Korngold and Sprent, 1983), GvL (Spierings et al, 2003;Mutis et al, 2002;Riddell et al, 2002;Nishida et al, 2004), and structural analyses of TCR-peptide/MHC interactions (Ostrov et al, 2002). H7 is one of the classical mH-Ag that was originally defined by Snell.…”

Identification of human minor histocompatibility antigens based on genetic association with highly parallel genotyping of pooled DNA

“…To evaluate the probability of a given minor H antigen being captured in high r 2 with marker SNPs, we checked the maximum r 2 values of known minor H antigen SNPs with the Affymetrix 500 K SNPs, according to empirical data from the HapMap project (www.hapmap.org). Among 13 known minor H antigens, 7 have their entries (designated minor H SNP) in the HapMap phase II SNP set (HA-3, 30 HA-8, 31 HB-1, 11 ACC-1 and ACC-2, 7 LB-ADIR-1F, 10 and 7A7-PANE1 13 ), and were used for this purpose (note that absence of their entries in the HapMap data set does not necessarily mean that they could not be captured by a particular marker SNP set). As shown in Table S4, all 7 minor H SNPs are captured by at least one flanking SNP that is included in the Affymetrix 500 K SNP set with r 2 values of more than 0.74 in at least one HapMap panel.…”

“…This incidentally provides a second example of products from both dichotomous SNP alleles being recognized as HLA-A*2402-restricted minor H antigens, the first example being the HB-1 minor H antigen. 24 Finally, real-time quantitative PCR revealed that T cells carrying the complementarity-determining region 3 sequence identical to CTL-1B9 became detectable in the patient's blood at the frequencies of 0.22%, 0.91%, 1.07% and 0.01% among TCR␣␤ ϩ T cells at days 30,102,196, and 395 after transplantation, respectively, suggesting that ACC-1 C minor H antigen is indeed immunogenic ( Figure 5D). …”