The minor collagens in articular cartilage

Luo, Yunyun; Sinkevičiūtė, Dovilė; He, Yi; Karsdal, Morten A.; Henrotin, Yves; Mobasheri, Ali; Önnerfjord, Patrik; Bay‐Jensen, Anne‐Christine

doi:10.1007/s13238-017-0377-7

Cited by 202 publications

(179 citation statements)

References 121 publications

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“…This collagen type was detected as upregulated in human skin keloid formation . The major function of collagen VI is probably as an anchoring meshwork connecting collagen fibers to the surrounding matrix, but the precise role of collagen VI in cartilage has not been clearly defined . Veidal et al suggested that collagen VI turnover is a central player in liver fibrogenesis .…”

Section: Discussionmentioning

confidence: 99%

“…24 The major function of collagen VI is probably as an anchoring meshwork connecting collagen fibers to the surrounding matrix, but the precise role of collagen VI in cartilage has not been clearly defined. 25 Veidal et al suggested that collagen VI turnover is a central player in liver fibrogenesis. 26 Moreover, the C- terminate peptide of collagen VI chain alpha 3 (endotrophin) is known to promote phenotypic modulation and proliferation of cells.…”

Section: Collagen IIImentioning

confidence: 99%

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Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Eckhardt

Novotný

Doubková

et al. 2019

Journal Orthopaedic Research

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Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side; n = 16), with non‐contracted tissue (lateral side; n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res

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Section: Discussionmentioning

confidence: 99%

Section: Collagen IIImentioning

confidence: 99%

Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Eckhardt

Novotný

Doubková

et al. 2019

Journal Orthopaedic Research

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“…Col X is highly expressed in hypertrophic chondrocytes found in OA cartilage (Luo et al 2017). Approximately 45% of collagens produced by hypertrophic chondrocytes are Col X (Shen 2005).…”

Section: Discussionmentioning

confidence: 99%

Human Platelet Lysate Promotes Proliferation but Fails to Maintain Chondrogenic Markers of Chondrocytes

Harto¹,

Mahmud²,

Othman³

et al. 2019

JSM

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Traditionally, foetal bovine serum (FBS) is used as a serum supplement for stem cell expansion in vitro. However, it is associated with xenoimmunisation and the transmission of animal pathogens, which may cause harm to stem cell recipients. As a safer alternative, human platelet lysate (HPL) has been introduced for propagating stem cells. Chondrocytes are expanded in vitro for cartilage repair via autologous chondrocyte implantation (ACI). In this study, we compare the efficacy of HPL prepared from expired platelet concentrates with that of FBS for promoting the proliferation and maintenance of the chondrogenic markers of primary human chondrocytes expanded in vitro. Chondrocytes were cultured in F12:Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% HPL, 10% HPL and 10% FBS. The cell morphology, viability and growth rate were examined from passage 1 (P1) to P3. RNA was isolated from P3 cells for quantitative polymerase chain reaction (qPCR) to determine the gene expression level of the chondrogenic, dedifferentiation and hypertrophic markers. HPL promoted chondrocyte proliferation without compromising cell viability. In addition, the chondrocytes cultured with HPL were smaller. However, HPL failed to maintain the chondrogenic markers, except SOX 9 (SRY-box transcription factor 9), which was upregulated, but not significantly. Nonetheless, HPL also suppressed the expression of type X collagen (Col X), a chondrocyte hypertrophic marker. In summary, we demonstrate the benefits of HPL supplementation in human chondrocyte culture, where it enhances cell proliferation and suppresses chondrocyte hypertrophy. In the future, HPL can be used for the large-scale expansion of chondrocytes for ACI. ABSTRAKSecara tradisinya, serum anak lembu (FBS) digunakan untuk pengkulturan sel in vitro. Namun demikian, FBS dikaitkan dengan penolakan oleh sistem imun dan penularan patogen haiwan yang berkemungkinan membawa kemudaratan kepada penerima sel induk. Lisat platelet manusia (HPL) telah diperkenalkan sebagai alternatif yang lebih selamat untuk pengkulturan sel. Sel kondrosit telah dikultur secara in vitro untuk merawat rawan yang rosak melalui teknik implantasi kondrosit autologus (ACI). Pada kajian ini, kami membandingkan keberkesanan HPL yang disediakan daripada platelet pekat tamat tempoh dan FBS dalam merangsang proliferasi dan mengekalkan penanda kondrogenik sel kondrosit manusia yang dikulturkan secara in vitro. Sel kondrosit dikultur dalam medium F12:DMEM yang ditambah dengan 5% HPL, 10% HPL dan 10% FBS. Morfologi, kadar kehidupan dan kadar pertumbuhan sel daripada P1 hingga P3 dikenal pasti. RNA dipencil daripada sel pada P3 untuk qPCR untuk menentukan ekspresi gen penanda-penanda kondrogenik, penyahbezaan dan hipertrofi. Hasil kajian mendapati HPL merangsang proliferasi sel kondrosit tanpa memkompromikan kadar kehidupan sel. Selain itu, sel kondrosit yang dikultur dengan HPL menunjukkan saiz yang lebih kecil. Namun demikian, HPL gagal mengekalkan penanda-penanda kondrogenik kecuali SOX 9 yang meningkat secara t...

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“…From these 43 proteins, 33 were significantly increased and 10 were significantly decreased. The upregulated proteins include several typical structural proteins of the cartilage ECM, including perlecan (33), the three alpha chains of type VI collagen (34) or tenascin (35), and also many different glycolytic enzymes, such as glyceraldehyde- Supplemental Table S2). We identified 35 metabolites in OA samples that showed significant discrimination between days 2 and 14 ( Figure 3, p < 0.05).…”

Section: Differentially Expressed Proteins During the Chondrogenesis mentioning

confidence: 99%

Integrative Metabolic Pathway Analysis Reveals Novel Therapeutic Targets in Osteoarthritis

Rocha

Cillero‐Pastor

Eijkel

et al. 2020

Molecular & Cellular Proteomics

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In osteoarthritis (OA), impairment of cartilage regeneration can be related to a defective chondrogenic differentiation of mesenchymal stromal cells (MSCs). Therefore, understanding the proteomic- and metabolomic-associated molecular events during the chondrogenesis of MSCs could provide alternative targets for therapeutic intervention. Here, a SILAC-based proteomic analysis identified 43 proteins related with metabolic pathways whose abundance was significantly altered during the chondrogenesis of OA human bone marrow MSCs (hBMSCs). Then, the level and distribution of metabolites was analyzed in these cells and healthy controls by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), leading to the recognition of characteristic metabolomic profiles at the early stages of differentiation. Finally, integrative pathway analysis showed that UDP-glucuronic acid synthesis and amino sugar metabolism were downregulated in OA hBMSCs during chondrogenesis compared with healthy cells. Alterations in these metabolic pathways may disturb the production of hyaluronic acid (HA) and other relevant cartilage extracellular matrix (ECM) components. This work provides a novel integrative insight into the molecular alterations of osteoarthritic MSCs and potential therapeutic targets for OA drug development through the enhancement of chondrogenesis.

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The minor collagens in articular cartilage

Cited by 202 publications

References 121 publications

Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Human Platelet Lysate Promotes Proliferation but Fails to Maintain Chondrogenic Markers of Chondrocytes

Integrative Metabolic Pathway Analysis Reveals Novel Therapeutic Targets in Osteoarthritis

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