The minimum substrate of cyclic AMP-stimulated protein kinase, as studied by synthetic peptides representing the phosphorylatable site of pyruvate kinase (type L) of rat liver

Zetterqvist, Örjan; Ragnarsson, Ulf; Humble, Elisabet; Berglund, Lars; Engström, Lorentz

doi:10.1016/0006-291x(76)90648-3

Cited by 210 publications

(57 citation statements)

References 9 publications

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“…Phosphorylation is known to occur at serine or threonine residues (38)(39)(40). In the case of phosphoserine, one or two basic amino acids are found close to the NH2-terminal side of this residue (38,39).…”

Section: Methodsmentioning

confidence: 99%

cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.

Kvist¹,

Brégégère²,

Rask³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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ABSTRACTmRNA coding for mouse major transplantation antigens ofthe d haplotype was partially purified, copied into double-stranded cDNA, and cloned in Escherichia coli. Clones were selected by their ability to hybridize specifically with mRNA coding for H-2K, D, or L antigens. One of these clones, pH-2d_1, carries a 1200-base-pair insert, comprising the noncoding region, including poly(A) at the 3' end and part ofthe coding region. A partial sequence of the latter region showed extensive homology with the known amino acid sequences of H-2Kb, Kk, and HLA-B7 antigens.From this comparison, it appears that the coding region extends from amino acid 133 in the second domain, through the third domain, to the cytoplasmic COOH.terminal region. A stretch of 24 hydrophobic or uncharged residues, located 31 amino acids from the COOH-terminal end, could represent the segment that spans the membrane. This is followed on the cytoplasmic side of the membrane by a cluster of basic amino acids and a possible phosphorylation site on a threonine residue.

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Section: Methodsmentioning

confidence: 99%

cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.

Kvist¹,

Brégégère²,

Rask³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…The in vitro phosphorylation reaction is performed in the presence of radiolabelled ATP, the array exposed to film and the image captured. Once the set of peptides have been synthesized, a large number of these microarrays can be made to screen many kinases relatively quickly (Kemp et al, 1975;Zetterqvist et al, 1976). The limitation of site-specific microarray is that, in vitro data is not sufficient on its own to definitively prove that a kinase may phosphorylate a given site in vivo.…”

Section: Site-specific Microarraysmentioning

confidence: 99%

Phosphoproteomics: Detection, Identification and Importance of Protein Phosphorylation

Jia¹,

Lin²,

Souchelnytskyi³

2012

Integrative Proteomics

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“…31 For example, the consensus site for the cAMP-dependent protein kinase (PKA) differs from that for protein kinase C or the Cdc2-like cyclin-dependent protein kinases. 29,[32][33][34] Candidate substrates for a given kinase …”

Section: Use Of Consensus Site Informationmentioning

confidence: 99%