The minimal enzyme cocktail concept for biomass processing

Meyer, Anne S.; Rosgaard, Lisa; Sørensen, Henrik Rokkjær

doi:10.1016/j.jcs.2009.01.010

Cited by 94 publications

(64 citation statements)

References 42 publications

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“…Prior to fermentation, the hemicellulose is pre-treated by acid hydrolysis or subjected to alkali treatment generating non-fermentable oligosaccharide by-products inhibiting the fermentation (Saha & Bothast, 1999). A promising alternative solution consists in enzymatic degradation of hemicelluloses using a cocktail of hemicellulolytic ABFs, xylanases, esterases and xylosidases (Harris et al, 2014), which importantly advances conversion of hemicellulose to monosaccharides (Meyer et al, 2009;Sørensen et al, 2003;Yang et al, 2014a,b). Araf and Xylp can be potent inducers for production of xylanolytic enzymes in T. reesei and a T. reesei mutant, when fed simultaneously with glucose and cellobiose gave high levels of cellulolytic and ABF activities (Ike et al, 2013).…”

Section: Biorefineriesmentioning

confidence: 99%

GH62 arabinofuranosidases: Structure, function and applications

Wilkens

Andersen

Dumon

et al. 2017

Biotechnology Advances

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Motivated by industrial demands and ongoing scientific discoveries continuous efforts are made to identify and create improved biocatalysts dedicated to plant biomass conversion. --1,3 arabinofuranosyl specific -L-arabinofuranosidases (EC 3.2.1.55) are debranching enzymes catalyzing hydrolytic release -L-arabinofuranosyl residues, which decorate xylan or arabinan backbones in lignocellulosic and pectin constituents of plant cell walls. The CAZy database classifies -L-arabinofuranosidases in Glycoside Hydrolase (GH) families GH2, GH3, GH43, GH51, GH54 and GH62. Only GH62 contains exclusively -L-arabinofuranosidases and these are of fungal and bacterial origin. Twenty-two GH62 enzymes out of 223 entries in the CAZy database have been characterized and very recently new knowledge was acquired with regard to crystal structures, substrate specificities, and phylogenetics, which overall provides novel insights into structure/function relationships of GH62. Overall GH62 -L-arabinofuranosidases are believed to play important roles in nature by acting in synergy with several cell wall degrading enzymes and members of GH62 represent promising candidates for biotechnological improvements of biofuel production and in various biorefinery applications.3

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Section: Biorefineriesmentioning

confidence: 99%

GH62 arabinofuranosidases: Structure, function and applications

Wilkens

Andersen

Dumon

et al. 2017

Biotechnology Advances

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“…One concept related to models for an optimised saccharification reaction is that of the minimal enzyme concept [175]. The minimal enzyme concept is defined by its authors as concerning the identification of the minimal number, minimal levels and the optimal combination of the best performing mono-active enzymes to saccharify lignocellulose [175].…”

Section: Interpreting Transcriptional Data To Guide Optimisation Of Smentioning

confidence: 99%

“…The minimal enzyme concept is defined by its authors as concerning the identification of the minimal number, minimal levels and the optimal combination of the best performing mono-active enzymes to saccharify lignocellulose [175]. There are arguments both in support of and opposing using what is transcriptionally induced and when for an optimised saccharification reaction.…”

Section: Interpreting Transcriptional Data To Guide Optimisation Of Smentioning

confidence: 99%

“…Based on levels of transcription and secretion, these cellobiohydrolases may be required in substantial amounts for an optimised saccharification reaction. Indeed, in an analysis determining the optimal amounts of four T. reesei proteins (CBH1, CBH2, EGL1 and EGL2) for the saccharification of pre-treated barley straw substrates, CBH1 was the protein required in the largest amount [175].…”

Section: Interpreting Transcriptional Data To Guide Optimisation Of Smentioning

confidence: 99%

“…In optimised saccharification reactions for the depolymerisation of some polymers, endo-acting activities are redundant. Meyer et al [175] showed that for a vinasse substrate (an industrial waste residue), which contains arabinoxylan with a degree of polymerisation (DP) of less than ~36, endo-1,4-β-xylanase was not required. β-xylosidase and two arabinofuranosidases were sufficient to depolymerise the arabinoxylan.…”

Section: Interpreting Transcriptional Data To Guide Optimisation Of Smentioning

confidence: 99%

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Transcriptional Regulation and Responses in Filamentous Fungi Exposed to Lignocellulose

2015

Mycology: Current and Future Developments

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Biofuels derived from lignocellulose are attractive alternative fuels but their production suffers from a costly and inefficient saccharification step that uses fungal enzymes. One route to improve this efficiency is to understand better the transcriptional regulation and responses of filamentous fungi to lignocellulose. Sensing and initial contact of the fungus with lignocellulose is an important aspect. Differences and similarities in the responses of fungi to different lignocellulosic substrates can partly be explained with existing understanding of several key regulators and their mode of action, as will be demonstrated for Trichoderma reesei, Neurospora crassa and Aspergillus spp. The regulation of genes encoding Carbohydrate Active enZymes (CAZymes) is influenced by the presence of carbohydrate monomers and short oligosaccharides, as well as the external stimuli of pH and light. We explore several important aspects of the response to lignocellulose that are not related to genes encoding CAZymes, namely the regulation of transporters, accessory proteins and stress responses. The regulation of gene expression is examined from the perspective of mixed cultures and models are presented for the nature of the transcriptional basis for any beneficial effects of such mixed cultures. Various applications in biofuel technology are based on manipulating transcriptional regulation and learning from fungal responses to lignocelluloses. Here we critically access the application of fungal transcriptional responses to industrial saccharification reactions. As part of this chapter, selected regulatory mechanisms are also explored in more detail.

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Production of fermentable sugars from sugarcane bagasse

Zhang

Harrison

O’Hara

2016

Sugarcane‐Based Biofuels and Bioproducts

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Sugar (or more specifically sucrose) is one of the major food carbohydrate energy sources in the world. It is used as a sweetener, preservative, and colorant in baked and processed foods and beverages and is one of lowest cost energy sources for human metabolism.On an industrial scale, sucrose is produced from two major crops -sugarcane, grown in tropical and subtropical regions of the world, and sugar beet, grown in more temperate climates. Sugarcane, however, accounts for the vast majority of global sugar production.For much of the history of sugarcane production, sugar was a scarce and highly valued commodity. Sugarcane processing focused on extracting sucrose as efficiently as possible for the lucrative markets in the United Kingdom and Europe. The potential for the production of alternative products from sugarcane, however, has long been recognized. The key process by-products including bagasse, molasses, mud, and ash have all been investigated as a basis for the production of alternative products (Rao 1997, Taupier andBugallo 2000).Sugarcane is believed to have originated in southern Asia, and migrated in several waves following trade routes through the Pacific to Oceania and Hawaii and through India into Europe. Sugarcane was introduced and spread through the Americas following the expansion by British, Spanish, and Portuguese colonies in the 15th and 16th centuries (Barnes 1964).While various methods of juice extraction and sugar production have been used over centuries to produce sugar, substantial innovations in sugar chemistry and processing technologies throughout the 18th and 19th centuries have formed the basis of modern sugar production methods (Bruhns et al. 1998). Dramatic improvements in processing efficiency, sugar quality, and automation and control characterized sugar processing throughout the 20th century.

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The minimal enzyme cocktail concept for biomass processing

Cited by 94 publications

References 42 publications

GH62 arabinofuranosidases: Structure, function and applications

GH62 arabinofuranosidases: Structure, function and applications

Transcriptional Regulation and Responses in Filamentous Fungi Exposed to Lignocellulose

Production of fermentable sugars from sugarcane bagasse

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