The Min DNA inversion enzyme of plasmid p15B of <i>Escherichia coli</i> 15T<sup>−</sup>: a new member of the Din family of site‐specific recombinases

Iida, Shigeru; Sandmeier, H.; Hübner, Philipp; Hiestand-Nauer, Rosemarie; Schneitz, Kay; Arber, Werner

doi:10.1111/j.1365-2958.1990.tb00671.x

Cited by 19 publications

(20 citation statements)

References 21 publications

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“…In the p15B plasmid, whose S gene is homologous to that of P1 in its posterior region, inversions can occur at six crossover sites within the corresponding invertible segment and may result in 240 isomeric configurations of this region (269,270). The inversions depend on the p15B invertase Min, which can functionally replace the Cin invertase of P1 (154).…”

Section: Vol 186 2004 Bacteriophage P1 7053mentioning

confidence: 99%

Genome of Bacteriophage P1

et al. 2004

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P1 is a bacteriophage of Escherichia coli and other enteric bacteria. It lysogenizes its hosts as a circular, low-copy-number plasmid. We have determined the complete nucleotide sequences of two strains of a P1 thermoinducible mutant, P1 c1-100. The P1 genome (93,601 bp) contains at least 117 genes, of which almost two-thirds had not been sequenced previously and 49 have no homologs in other organisms. Protein-coding genes occupy 92% of the genome and are organized in 45 operons, of which four are decisive for the choice between lysis and lysogeny. Four others ensure plasmid maintenance. The majority of the remaining 37 operons are involved in lytic development. Seventeen operons are transcribed from 70 promoters directly controlled by the master phage repressor C1. Late operons are transcribed from promoters recognized by the E. coli RNA polymerase holoenzyme in the presence of the Lpa protein, the product of a C1-controlled P1 gene. Three species of P1-encoded tRNAs provide differential controls of translation, and a P1-encoded DNA methyltransferase with putative bifunctionality influences transcription, replication, and DNA packaging. The genome is particularly rich in Chi recombinogenic sites. The base content and distribution in P1 DNA indicate that replication of P1 from its plasmid origin had more impact on the base compositional asymmetries of the P1 genome than replication from the lytic origin of replication.

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Section: Vol 186 2004 Bacteriophage P1 7053mentioning

confidence: 99%

Genome of Bacteriophage P1

et al. 2004

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“…The Gin protein of bacteriophage Mu, which is responsible for the switch in host range specificity of the phage (13,35), is a member of a family of highly homologous invertases including Hin of Salmonella typhimurium (39), Cin of phage P1 (7), Pin of the Escherichia coli phage e14 (26,37), and Min of E. coli plasmid p15B (8). This family of invertases is closely related to the family of resolvases including the resolvases of ␥␦ and Tn3 (27).…”

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Gin mutants that can be suppressed by a Fis-independent mutation

et al. 1995

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The Gin invertase of bacteriophage Mu mediates recombination between two inverted gix sites. Recombination requires the presence of a second protein, Fis, which binds to an enhancer sequence. We have isolated 24 different mutants of Gin that are impaired in DNA inversion but proficient in DNA binding. Six of these mutants could be suppressed for inversion by introduction of a second mutation, which when present in the wild-type gin gene causes a Fis-independent phenotype. Only one of the six resulting double mutants shows an inversion efficiency which is comparable to that of the wild-type Gin and which is independent of Fis. The corresponding mutation, M to I at position 108 (M108I), is located in a putative ␣-helical structure, which in the homologous ␥␦ resolvase has been implicated in dimerization. The properties of the M108I mutant suggest that in Gin this dimerization helix might also be the target for Fis interaction. The five other mutants that show a restored inversion after introduction of a Fis-independent mutation appear to be completely dependent on Fis for this inversion. The corresponding mutations are located in different domains of the protein. The properties of these mutants in connection with the role of Fis in inversion will be discussed.

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“…These two families are evolutionarily and mechanistically distinct, and both contain members with diverse functions including transposases, integrases, and DNA invertases. DNA invertases of the Ssr family are typically encoded by elements imported to bacteria from a phage (10)(11)(12) or plasmid (13) and act locally on the imported element. Many DNA invertases of the Tsr family have been shown to regulate phase variation of fimbriae by inverting a single area of the chromosome adjacent to the Tsr gene (14)(15)(16).…”

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Mpi recombinase globally modulates the surface architecture of a human commensal bacterium

Coyne

Weinacht²,

Krinos³

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

114

116

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The mammalian gut represents a complex and diverse ecosystem, consisting of unique interactions between the host and microbial residents. Bacterial surfaces serve as an interface that promotes and responds to this dynamic exchange, a process essential to the biology of both symbionts. The human intestinal microorganism, Bacteroides fragilis, is able to extensively modulate its surface. Analysis of the B. fragilis genomic sequence, together with genetic conservation analyses, cross-species cloning experiments, and mutational studies, revealed that this organism utilizes an endogenous DNA inversion factor to globally modulate the expression of its surface structures. This DNA invertase is necessary for the inversion of at least 13 regions located throughout the genome, including the promoter regions for seven of the capsular polysaccharide biosynthesis loci, an accessory polysaccharide biosynthesis locus, and five other regions containing consensus promoter sequences. Bacterial DNA invertases of the serine site-specific recombinase family are typically encoded by imported elements such as phage and plasmids, and act locally on a single region of the imported element. In contrast, the conservation and unique global regulatory nature of the process in B. fragilis suggest an evolutionarily ancient mechanism for surface adaptation to the changing intestinal milieu during commensalism.

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The Min DNA inversion enzyme of plasmid p15B of Escherichia coli 15T⁻: a new member of the Din family of site‐specific recombinases

Cited by 19 publications

References 21 publications

Genome of Bacteriophage P1

Genome of Bacteriophage P1

Gin mutants that can be suppressed by a Fis-independent mutation

Mpi recombinase globally modulates the surface architecture of a human commensal bacterium

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The Min DNA inversion enzyme of plasmid p15B of Escherichia coli 15T−: a new member of the Din family of site‐specific recombinases

Cited by 19 publications

References 21 publications

Genome of Bacteriophage P1

Genome of Bacteriophage P1

Gin mutants that can be suppressed by a Fis-independent mutation

Mpi recombinase globally modulates the surface architecture of a human commensal bacterium

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Product

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The Min DNA inversion enzyme of plasmid p15B of Escherichia coli 15T⁻: a new member of the Din family of site‐specific recombinases