The migration speed of cancer cells influenced by macrophages and myofibroblasts co-cultured in a microfluidic chip

Hsu, Tsi-Hsuan; Kao, Y. L.; Lin, W. L.; Xiao, Jian-Long; Kuo, P. C.; Wu, Chih-Wei; Liao, Wen-Chieh; Lee, Chau‐Hwang

doi:10.1039/c2ib00112h

Cited by 39 publications

(28 citation statements)

References 26 publications

(27 reference statements)

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“…Interestingly, instead of pretreatment, direct combination of macrophage conditioned medium and myofibroblast conditioned medium resulted in a nearly threefold increase of lung cancer cells' migration speed [167]. Similar to other triculture microfluidic models, these results imply that the responses of cancer cells influenced under multiple factors can be quite complex and diverse [155,167].…”

Section: Metastatic Niche: Modelling Tumour -Stroma Interaction and Msupporting

confidence: 57%

“…Similar to other triculture microfluidic models, these results imply that the responses of cancer cells influenced under multiple factors can be quite complex and diverse [155,167].…”

Section: Metastatic Niche: Modelling Tumour -Stroma Interaction and Msupporting

confidence: 55%

“…Small vesicles containing nucleic acids and proteins (termed exosomes) may be the carriers to carry signalling molecules between the cancer and stromal cells [178]. Hsu et al [167] developed a two-dimensional three-chamber PDMS microfluidic chip with microvalve control to selectively flow the conditioned media of fibroblast, macrophages and CL1-0 lung cancer cells to investigate how paracrine signalling from tumour stroma affected cancer cell invasiveness (figure 6d). Lung cancer cells release TGF-b1 to transform fibroblasts into myofibroblasts and in return promote the migration speed of cancer cells.…”

Section: Metastatic Niche: Modelling Tumour -Stroma Interaction and Mmentioning

confidence: 99%

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Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

Tsai

Trubelja

Shen

et al. 2017

J. R. Soc. Interface.

172

133

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Cancer remains one of the leading causes of death, albeit enormous efforts to cure the disease. To overcome the major challenges in cancer therapy, we need to have a better understanding of the tumour microenvironment (TME), as well as a more effective means to screen anti-cancer drug leads; both can be achieved using advanced technologies, including the emerging tumour-on-a-chip technology. Here, we review the recent development of the tumour-on-a-chip technology, which integrates microfluidics, microfabrication, tissue engineering and biomaterials research, and offers new opportunities for building and applying functional three-dimensional in vitro human tumour models for oncology research, immunotherapy studies and drug screening. In particular, tumour-on-a-chip microdevices allow well-controlled microscopic studies of the interaction among tumour cells, immune cells and cells in the TME, of which simple tissue cultures and animal models are not amenable to do. The challenges in developing the next-generation tumour-on-a-chip technology are also discussed.

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Section: Metastatic Niche: Modelling Tumour -Stroma Interaction and Msupporting

confidence: 57%

“…Similar to other triculture microfluidic models, these results imply that the responses of cancer cells influenced under multiple factors can be quite complex and diverse [155,167].…”

Section: Metastatic Niche: Modelling Tumour -Stroma Interaction and Msupporting

confidence: 55%

Section: Metastatic Niche: Modelling Tumour -Stroma Interaction and Mmentioning

confidence: 99%

See 1 more Smart Citation

Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

Tsai

Trubelja

Shen

et al. 2017

J. R. Soc. Interface.

172

133

View full text Add to dashboard Cite

show abstract

“…In our opinion, the overlook of tumor microenvironment has inescapable relationship with tough problem. In vivo, cancer cells were cultured in dishes in conventional methods such as transwell assay 16 or bathing cells in conditioned medium 17 , but with poor in vivo-like microenvironment reconstruction 18,19 . In the present study, a 3D microfluidic device was developed to reconstruct an in vivo-like tumor microenvironment for bladder cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Although a few methods such as transwell assay 16 or bathing cells in conditioned medium 17 were proposed to study the interactions between cancer cells and stromal cells, most of them are still limited because of the poor in vivo-like microenvironment reconstruction 18,19 . Recently, microfluidics has attracted intensive attention in molecular and cellular biology research due to its miniaturized size, high throughput detection, low consumption of reagents and especially the accurate reconstruction of 3D in vivo-like microenvironment 1,13 .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device

et al. 2015

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Stromal fibroblasts are essential for tumor proliferation and invasion. Here we presented a 3-dimensional (3D) microfluidic co-culture device to reconstruct an in vivo-like tumor microenvironment for investigation of the interactions of cancer-associated fibroblasts (CAFs) and bladder cancer cells. With this device, we verified that the cytokines secreted by bladder cancer cells T24 effectively transform the fibroblasts into CAFs. Compared to fibroblasts, the CAFs, which undergo the aerobic glycolysis, showed higher ability to produce lactate and provide energy for bladder cancer cell proliferation and invasion. We also demonstrated that this kind of tumor-promoting effect was associated with the upregulation of monocarboxylate anion transporter 1 (MCT1) and MCT4 expression in CAFs. We concluded that MCT1 and MCT4 are involved in bladder cancer cell proliferation and invasiveness. Moreover, this 3D microfluidic co-culture device allows for the assay to characterize various cellular events in a single device sequentially, facilitating a better understanding of the interactions among heterotypic cells in a sophisticated microenvironment.

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Ex Vivo Tumor‐on‐a‐Chip Platforms to Study Intercellular Interactions within the Tumor Microenvironment

Kumar

Varghese

2018

Adv Healthcare Materials

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The emergence of immunotherapies and recent FDA approval of several of them makes them a promising therapeutic strategy for cancer. While these advancements underscore the potential of engaging the immune system to target tumors, this approach has so far been efficient only for certain cancers. Extending immunotherapy as a widely acceptable treatment for various cancers requires a deeper understanding of the interactions of tumor cells within the tumor microenvironment (TME). The immune cells are a key component of the TME, which also includes other stromal cells, soluble factors, and extracellular matrix‐based cues. While in vivo studies function as a gold standard, tissue‐engineered microphysiological tumor models can offer patient‐specific insights into cancer‐immune interactions. These platforms, which recapitulate cellular and non‐cellular components of the TME, enable a systematic understanding of the contribution of each component toward disease progression in isolation and in concert. Microfluidic‐based microphysiological platforms recreating these environments, also known as “tumor‐on‐a‐chip,” are increasingly being utilized to study the effect of various elements of TME on tumor development. Herein are reviewed advancements in tumor‐on‐a‐chip technology that are developed and used to understand the interaction of tumor cells with other surrounding cells, including immune cells, in the TME.

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The migration speed of cancer cells influenced by macrophages and myofibroblasts co-cultured in a microfluidic chip

Cited by 39 publications

References 26 publications

Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device

Ex Vivo Tumor‐on‐a‐Chip Platforms to Study Intercellular Interactions within the Tumor Microenvironment

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