The Middlebrook--Dubos Haemagglutination Test and Its Haemolytic Modification in the Diagnosis of Tuberculosis

Sherris, John C.

doi:10.1136/jcp.6.1.64

Cited by 10 publications

(3 citation statements)

References 18 publications

(6 reference statements)

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“…normal guinea pig plasma. The hemagglutination test was then done according to the protocol of Sherris (35), with the exception that Lederle 4X OT was used. No heat lability of this antibody was observed.…”

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Correlations Between Plasma Protein Fractions, Antibody Titers, and the Passive Transfer of Delayed and Immediate Cutaneous Reactivity to Tuberculin PPD and Tuberculopolysaccharides

Cole

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1955

The Journal of Experimental Medicine

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Delayed or tuberculin type sensitivity is characterized by the progressive development of an inflammatory reaction at the site of tuberculin deposits. This reaction reaches its peak after 24 hours and when intense is marked by tissue necrosis. The term, delayed, best applies to the gross appearance of the reaction since time lapse movies of this process (1) indicate that the reaction begins after a few minutes. Within several hours the gross reaction is marked by induration, erythema, and some edema. Histological studies of these changes, according to Dienes and Mallory (2), are characterized by a predominant round cell infiltration, vascular engorgement, and by variable amounts of polymorphonuclear infiltration. In contradistinction, Follis (3) found that polymorphonuclear leucocytes predominate.Many efforts to show that this type response is due to a circulating antibody have been unsuccessful. Passive transfer of tuberculin type allergy by serum has been reported only once (4). Rich and Lewis found that tissue cultures of spleen, bone marrow, and peripheral blood cells from animals sensitized to tuberculin were injured by high concentrations of tuberculin (5). Moen and Swift (6) found that this cellular sensitivity is disease-specific. These in vitro observations have been the basis for the befief that delayed type allergy is cellular rather than humoral in the usual sense applied to immune responses.There are certain features of tissue culture studies on tuberculin sensitivity which "should be mentioned. Not all workers have been able to repeat these findings (7). Cultures of dermal cells (8) are quite insensitive to tuberculin in extraordinary amounts compared to those which cause brisk cutaneous tuberculin reactions. A similar lack of correlation between degrees of cutaneous sensitivity in the host and the noxious effect of tuberculin in

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Section: $4 and $6mentioning

confidence: 99%

Correlations Between Plasma Protein Fractions, Antibody Titers, and the Passive Transfer of Delayed and Immediate Cutaneous Reactivity to Tuberculin PPD and Tuberculopolysaccharides

Cole

Favour

1955

The Journal of Experimental Medicine

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“…These have included more thorough absorption of sera with normal sheep erythrocytes to remove heterophile antibodies (17), the substitution of human Group 0 cells for sheep erythrocytes (18)(19)(20)(21), the use of fowl erythrocytes (22), and the use of collodion particles (23). Middlebrook (24) introduced a hemolytic modification which has received considerable study (25)(26)(27)(28)(29)(30)(31)(32)(33). A slide agglutination test has been used (34).…”

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“…A slide agglutination test has been used (34). Different products of the tubercle bacillus have been used (1, 5, 15,20,23,29,33,35,36) in attempts to make the test more specific, and modifications of the method of adsorbing antigens on the erythrocytes have included the use of tannic acid (37)(38)(39), inulin or trypsin (38), and treatment of the cells with Forssman antibody (30). In an effort to explain false negative reactions, "incomplete" or "blocking" antibodies have been looked for by the use of such diluents as pooled serum, 5 per cent bovine albumin, glycine solution, or polyvinyl pyrrolidone (30), and by the use of the Coombs antiglobulin technique (26,40).…”

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The Middlebrook-Dubos Hemagglutination Test for Tuberculosis in Children and Adults1

Josiukas¹,

Roy²,

Boyd³

1954

J. Clin. Invest.

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A comparison of erythrocyte‐adsorbable antigens derived from different strains of human‐type tubercle bacilli

Salmeron¹,

Sherris²

1957

J. Pathol.

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The Middlebrook--Dubos Haemagglutination Test and Its Haemolytic Modification in the Diagnosis of Tuberculosis

Cited by 10 publications

References 18 publications

Correlations Between Plasma Protein Fractions, Antibody Titers, and the Passive Transfer of Delayed and Immediate Cutaneous Reactivity to Tuberculin PPD and Tuberculopolysaccharides

Correlations Between Plasma Protein Fractions, Antibody Titers, and the Passive Transfer of Delayed and Immediate Cutaneous Reactivity to Tuberculin PPD and Tuberculopolysaccharides

The Middlebrook-Dubos Hemagglutination Test for Tuberculosis in Children and Adults1

A comparison of erythrocyte‐adsorbable antigens derived from different strains of human‐type tubercle bacilli

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